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AB125243

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]

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(34 Publications)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) is a mouse monoclonal antibody detecting DDDDK tag (Binds to FLAG® tag sequence) in Western Blot, IP, ELISA, Dot Blot, ISH.

- Over 30 publications

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

1 Images
Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB125243)
  • WB

Unknown

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB125243)

All lanes:

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) at 1/1000 dilution

Lane 1:

HEK293 cell lysate non-transfected

Lane 2:

HEK293 cell lysate transfected with DDDDK tagged protein vector

true

  • Biotin

    Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FG4R

Isotype

IgG2b

Carrier free

No

Applications

Dot, ELISA, ISH, IP, WB

applications

Specificity

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ISH" : {"fullname" : "In Situ Hybridisation", "shortname":"ISH"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ISH-species-checked": "guaranteed", "ISH-species-dilution-info": "1/500 - 1/2000", "ISH-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p>(with ECL)</p>" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab125243 was purified by protein A affinity chromatography from TCS
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)
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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4776 PubMed37553334

2023

Structure, catalysis, chitin transport, and selective inhibition of chitin synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Chen,Zhao-Bin Wang,Le-Xuan Wang,Peng Zhao,Cai-Hong Yun,Lin Bai

Plant biotechnology journal 21:1191-1205 PubMed36786225

2023

Biotinylated Tn5 transposase-mediated CUT&Tag efficiently profiles transcription factor-DNA interactions in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yuan Tao,Xue-Ying Guan,Gao-Jie Hong,Yu-Qing He,Su-Juan Li,Shou-Li Feng,Jian Wang,Guang Chen,Fei Xu,Jia-Wei Wang,Sheng-Chun Xu

The Journal of biological chemistry 299:102971 PubMed36736423

2023

Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Wenjun Song,Xinying Ma,Mingxiao Yu,Lulu Chen,Yanling Tao

Nature communications 13:7629 PubMed36494373

2022

Engineering antiviral immune-like systems for autonomous virus detection and inhibition in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yidan Wang,Ying Xu,Chee Wah Tan,Longliang Qiao,Wan Ni Chia,Hongyi Zhang,Qin Huang,Zhenqiang Deng,Ziwei Wang,Xi Wang,Xurui Shen,Canyu Liu,Rongjuan Pei,Yuanxiao Liu,Shuai Xue,Deqiang Kong,Danielle E Anderson,Fengfeng Cai,Peng Zhou,Lin-Fa Wang,Haifeng Ye

EBioMedicine 85:104278 PubMed36202053

2022

SIRPα maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection and through autophagy and necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wang,Yunkai Lin,Feihong Xu,Hui Zhang,Xiaoyan Zhu,Zhen Liu,Yuan Hu,Guanjun Dong,Bingqi Sun,Yanhong Yu,Guoren Ma,Zhigang Tang,Diana Legarda,Adrian Ting,Yuan Liu,Jia Hou,Liwei Dong,Huabao Xiong

Frontiers in microbiology 13:911479 PubMed35770165

2022

The Effector Protein CgNLP1 of Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyong Yang,Jie Yang,Qiwei Zhang,Wenfeng Wang,Liping Feng,Li Zhao,Bang An,Qiannan Wang,Chaozu He,Hongli Luo

Stem cells international 2022:3632549 PubMed35126526

2022

Chidamide Suppresses the Growth of Cholangiocarcinoma by Inhibiting HDAC3 and Promoting FOXO1 Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongpan Li,Jujia Zheng,Qiang Huo,Zhongchao Chen,Jun Chen,Xiangwei Xu

Frontiers in microbiology 12:762869 PubMed34992585

2021

Enterovirus A71 2B Inhibits Interferon-Activated JAK/STAT Signaling by Inducing Caspase-3-Dependent Karyopherin-α1 Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Menghuai Sun,Qian Lin,Chunyang Wang,Jiao Xing,Kunlong Yan,Zhifeng Liu,Yu Jin,Carol J Cardona,Zheng Xing

Bone research 9:50 PubMed34876557

2021

Runx1 protects against the pathological progression of osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenchen Zhou,Yujia Cui,Yueyi Yang,Daimo Guo,Demao Zhang,Yi Fan,Xiaobing Li,Jing Zou,Jing Xie

Cell death discovery 7:372 PubMed34857740

2021

PIAS1 alleviates diabetic peripheral neuropathy through SUMOlation of PPAR-γ and miR-124-induced downregulation of EZH2/STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Zixin Hou,Ji Chen,Huan Yang,Xiaoling Hu,Fengrui Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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