AB125243

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]

Lab Essentials
What is this?

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(53 Publications)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) is a mouse monoclonal antibody detecting DDDDK tag (Binds to FLAG® tag sequence) in Western Blot, IP, ELISA, Dot Blot, ISH.

- Over 30 publications

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

1 Images

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Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB125243)

All lanes:

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) at 1/1000 dilution

Lane 1:

HEK293 cell lysate non-transfected

Lane 2:

HEK293 cell lysate transfected with DDDDK tagged protein vector

true

Biotin

Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FG4R

Isotype

IgG2b

Carrier free

Applications

ELISA, IP, WB, ISH, Dot

applications

Specificity

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ISH" : {"fullname" : "In Situ Hybridisation", "shortname":"ISH"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ISH-species-checked": "guaranteed", "ISH-species-dilution-info": "1/500 - 1/2000", "ISH-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "(with ECL)" } } }

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Product details

What is this antibody validated in?
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ELISA, Dot Blot, In Situ Hybridisation. Tagged protein detection
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-DDDDK tag (Binds to FLAG® tag sequence) [FG4R] (ab125243) was first used in a scientific publication in 2012 and has been cited over 30 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [FG4R] also available for your convenience: ab125243, Biotin - ab173832

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab125243 was purified by protein A affinity chromatography from TCS

Storage buffer

pH: 7.2 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (53)

Recent publications for all applications. Explore the full list and refine your search

Clinical & translational immunology 14:e70048 PubMed40672112

2025

SIRT7 ameliorates Th17/Treg imbalance by desuccinylation of STAT3 to improve immune thrombocytopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Ge,Xiaoyan Zhang,Fajuan Tang,Yan Liu

Journal of orthopaedic surgery and research 20:517 PubMed40420098

2025

TRIM55 suppresses inflammatory response after spinal cord injury by accelerating the ubiquitination and degradation of TLR4.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li

Research (Washington, D.C.) 8:0703 PubMed40416363

2025

Targeting the Exonic Circular RNA/O-GlcNAc Transferase/Forkhead Box C1 Axis Inhibits Asparagine- and Alanine-Mediated Ferroptosis Repression in Neuroblastoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qilan Li,Yang Cheng,Chunhui Yang,Minxiu Tian,Xiaojing Wang,Dan Li,Xinyue Li,Jiaying Qu,Shunchen Zhou,Liduan Zheng,Qiangsong Tong

Orphanet journal of rare diseases 20:121 PubMed40075430

2025

Identification of ETFDH gene c. 487 + 2 T > A pathogenic variant and mechanisms for polycystic kidney in neonatal onset MADD.

Applications

Unspecified application

Species

Unspecified reactive species

Bijun Zhang,Dongyang Zhang,Feiyue Sun,Xinxin Si,Meng Luan,Rong He

Cell death & disease 16:144 PubMed40021626

2025

Lactylation-driven transcriptional activation of FBXO33 promotes gallbladder cancer metastasis by regulating p53 polyubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenheng Wu,You Peng,Wen Chen,Feng Xia,Tieshan Song,Qiming Ke

Oncogene 44:1400-1414 PubMed40011576

2025

Cancer-associated fibroblasts promote EGFR-TKI resistance via the CTHRC1/glycolysis/H3K18la positive feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Wenxin Zhou,Hai Xu,Jiali Xu,Jun Li,Xinyin Liu,Xiyi Lu,Jiali Dai,Yuqin Jiang,Wei Wang,Erbao Zhang,Renhua Guo

The EMBO journal 44:1414-1441 PubMed39875724

2025

Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Huang,Hua Lu,Yao Liu,Jiabei Wang,Qingan Xia,Xiangmin Shi,Yan Jin,Xiaolin Liang,Wei Wang,Xiaopeng Ma,Yangyi Wang,Meng Gong,Canjun Li,Chunlei Cang,Qinghua Cui,Ceshi Chen,Tao Shen,Lianxin Liu,Xiangting Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2405492 PubMed39527694

2024

PcWRKY1 Represses Transcription of Yellow Stripe-Like 3 (PcYSL3) to Negatively Regulate Radial Cadmium Transport in Poplar Stems.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Chen,Yuhong Zhang,Yao Cheng,Wenjian Yu,Lingyu Yang,Peiqi Shu,Jing Zhou,Payam Fayyaz,Zhi-Bin Luo,Shurong Deng,Wenguang Shi

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Virulence 15:2368080 PubMed38899573

2024

Screening optimal DC-targeting peptide to enhance the immune efficacy of recombinant expressing RHDV VP60.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Xia,Xiao Lu,Deming Kong,Tiantian Guo,Yueyi Gao,Lingxiang Xin,Yanping Jiang,Xiaona Wang,Zhifu Shan,Jiaxuan Li,Han Zhou,Wen Cui,Xinyuan Qiao,Lijie Tang,Yijing Li,Li Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]

Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB125243)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (53)

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