Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody. Suitable for IP, ISH, ELISA, Dot, WB and reacts with Tag samples. Cited in 34 publications.
Images
![Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB125243), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-fg4r-ab125243--western-blot-img7293.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (AB125243)")
Publications
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- Nature communications 14:47762023Structure, catalysis, chitin transport, and selective inhibition of chitin synthase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan-Dan Chen,Zhao-Bin Wang,Le-Xuan Wang,Peng Zhao,Cai-Hong Yun,Lin BaiPubMed 37553334
- Plant biotechnology journal 21:1191-12052023Biotinylated Tn5 transposase-mediated CUT&Tag efficiently profiles transcription factor-DNA interactions in plants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Yuan Tao,Xue-Ying Guan,Gao-Jie Hong,Yu-Qing He,Su-Juan Li,Shou-Li Feng,Jian Wang,Guang Chen,Fei Xu,Jia-Wei Wang,Sheng-Chun XuPubMed 36786225
- The Journal of biological chemistry 299:1029712023Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zhang,Wenjun Song,Xinying Ma,Mingxiao Yu,Lulu Chen,Yanling TaoPubMed 36736423
- Nature communications 13:76292022Engineering antiviral immune-like systems for autonomous virus detection and inhibition in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYidan Wang,Ying Xu,Chee Wah Tan,Longliang Qiao,Wan Ni Chia,Hongyi Zhang,Qin Huang,Zhenqiang Deng,Ziwei Wang,Xi Wang,Xurui Shen,Canyu Liu,Rongjuan Pei,Yuanxiao Liu,Shuai Xue,Deqiang Kong,Danielle E Anderson,Fengfeng Cai,Peng Zhou,Lin-Fa Wang,Haifeng YePubMed 36494373
- EBioMedicine 85:1042782022SIRPα maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection and through autophagy and necroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Wang,Yunkai Lin,Feihong Xu,Hui Zhang,Xiaoyan Zhu,Zhen Liu,Yuan Hu,Guanjun Dong,Bingqi Sun,Yanhong Yu,Guoren Ma,Zhigang Tang,Diana Legarda,Adrian Ting,Yuan Liu,Jia Hou,Liwei Dong,Huabao XiongPubMed 36202053
- Frontiers in microbiology 13:9114792022The Effector Protein CgNLP1 of Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangyong Yang,Jie Yang,Qiwei Zhang,Wenfeng Wang,Liping Feng,Li Zhao,Bang An,Qiannan Wang,Chaozu He,Hongli LuoPubMed 35770165
- Stem cells international 2022:36325492022Chidamide Suppresses the Growth of Cholangiocarcinoma by Inhibiting HDAC3 and Promoting FOXO1 Acetylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongpan Li,Jujia Zheng,Qiang Huo,Zhongchao Chen,Jun Chen,Xiangwei XuPubMed 35126526
- Frontiers in microbiology 12:7628692021Enterovirus A71 2B Inhibits Interferon-Activated JAK/STAT Signaling by Inducing Caspase-3-Dependent Karyopherin-α1 Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMenghuai Sun,Qian Lin,Chunyang Wang,Jiao Xing,Kunlong Yan,Zhifeng Liu,Yu Jin,Carol J Cardona,Zheng XingPubMed 34992585
- Bone research 9:502021Runx1 protects against the pathological progression of osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenchen Zhou,Yujia Cui,Yueyi Yang,Daimo Guo,Demao Zhang,Yi Fan,Xiaobing Li,Jing Zou,Jing XiePubMed 34876557
- Cell death discovery 7:3722021PIAS1 alleviates diabetic peripheral neuropathy through SUMOlation of PPAR-γ and miR-124-induced downregulation of EZH2/STAT3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZixin Hou,Ji Chen,Huan Yang,Xiaoling Hu,Fengrui YangPubMed 34857740
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IP | ISH | ELISA | Dot | WB | |
|---|---|---|---|---|---|
| Tag | Expected | Expected | Expected | Expected | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
ISH
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/1000.00000 - 1/3000.00000 | Notes (with ECL) |
Associated Products
Select an associated product type
15 products for Alternative Product
Alternative names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Recommended products
Mouse Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody. Suitable for IP, ISH, ELISA, Dot, WB and reacts with Tag samples. Cited in 34 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- FG4R
- Purification technique
- Affinity purification Protein A
- Specificity
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.
- Concentration
- Purification notes
ab125243 was purified by protein A affinity chromatography from TCS
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
If stored at 4°C please add 0.1% sodium azide.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243), expandable thumbnail](https://content.abcam.com/products/images/ddddk-tag-binds-to-flag-tag-sequence-antibody-fg4r-ab125243--western-blot-img7293.jpg "Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)")
Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243)
All lanes: Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab125243) at 1/1000 dilution
Lane 1: HEK293 cell lysate non-transfected
Lane 2: HEK293 cell lysate transfected with DDDDK tagged protein vector
Developed using the ECL technique.
Downloads
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