AB151078

Anti-DDHD1 antibody

Name: Anti-DDHD1 antibody (ab151078) | Abcam
Brand: Abcam Limited
SKU: AB151078
Price: 645 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DDHD1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDHD1 aa 400 to C-terminus.

View Alternative Names

KIAA1705, DDHD1, Phospholipase DDHD1, DDHD domain-containing protein 1, Phosphatidic acid-preferring phospholipase A1 homolog, Phospholipid sn-1 acylhydrolase, PA-PLA1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling DDHD1 with ab151078 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling DDHD1 with ab151078 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling DDHD1 with ab151078 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling DDHD1 with ab151078 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-DDHD1 antibody (AB151078)

All lanes:

Western blot - Anti-DDHD1 antibody (ab151078) at 0.4 µg/mL

All lanes:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DDHD1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NEL9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDHD1 protein also known as DDH1 or PA-PLA1 is a phospholipase involved in lipid metabolism. With a molecular mass of approximately 88 kDa this protein localizes primarily in the cytosol but can also associate with the Golgi apparatus. DDHD1 is expressed in various tissues with significant levels in the brain and testis. This expression pattern suggests its potential role in central nervous system functions and reproductive processes.

Biological function summary

DDHD1 enzyme is responsible for the hydrolysis of phosphatidic acid contributing to lysophospholipid production. It is part of the intracellular membrane trafficking system operating independently rather than as part of a complex. By modulating the lipid composition of membranes DDHD1 helps regulate membrane curvature and dynamics which affects cellular processes such as vesicular transport and signaling.

Pathways

DDHD1 plays a significant role in the phospholipid signaling pathway by acting on phosphatidic acids. Its activity influences the levels of signaling molecules like lysophosphatidic acid which impact cellular events like proliferation and migration. Additionally DDHD1 is involved in the arachidonic acid pathway connecting it with enzymes such as PLA2 which also mediate the release of fatty acids from phospholipids essential for inflammatory responses.

DDHD1 mutations have been associated with hereditary spastic paraplegia a neurodegenerative condition characterized by progressive weakness and spasticity of the lower limbs. This relationship indicates its contribution to maintaining neuronal integrity. Furthermore DDHD1 function has links to mitochondrial lipid homeostasis associating it with DDHD2 in the context of complex lipid synthesis disorders especially those affecting the nervous system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Phospholipase A1 (PLA1) that hydrolyzes ester bonds at the sn-1 position of glycerophospholipids producing a free fatty acid and a lysophospholipid (Probable) (PubMed : 20359546, PubMed : 22922100). Prefers phosphatidate (1,2-diacyl-sn-glycero-3-phosphate, PA) as substrate in vitro, but can efficiently hydrolyze phosphatidylinositol (1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol), PI), as well as a range of other glycerophospholipid substrates such as phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE), phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine, PS) and phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol), PG) (Probable) (PubMed : 20359546). Involved in the regulation of the endogenous content of polyunsaturated PI and PS lipids in the nervous system. Changes in these lipids extend to downstream metabolic products like PI phosphates PIP and PIP2, which play fundamental roles in cell biology (By similarity). Regulates mitochondrial morphology (PubMed : 24599962). These dynamic changes may be due to PA hydrolysis at the mitochondrial surface (PubMed : 24599962). May play a regulatory role in spermatogenesis or sperm function (PubMed : 24599962).

See full target information DDHD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Angiogenesis 18:361-71 PubMed26018928

2015

Genome-wide expression analysis of wounded skin reveals novel genes involved in angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Brönneke,Bodo Brückner,Jörn Söhle,Ralf Siegner,Christoph Smuda,Franz Stäb,Horst Wenck,Ludger Kolbe,Elke Grönniger,Marc Winnefeld

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDHD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD1 antibody (AB151078)

Western blot - Anti-DDHD1 antibody (AB151078)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise