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AB241975

Anti-DDHD1 antibody

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(1 Publication)

Rabbit Polyclonal DDHD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDHD1 aa 50-100.

View Alternative Names

KIAA1705, DDHD1, Phospholipase DDHD1, DDHD domain-containing protein 1, Phosphatidic acid-preferring phospholipase A1 homolog, Phospholipid sn-1 acylhydrolase, PA-PLA1

2 Images
Immunoprecipitation - Anti-DDHD1 antibody (AB241975)
  • IP

Supplier Data

Immunoprecipitation - Anti-DDHD1 antibody (AB241975)

DDHD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241975 at 20 μl/reaction. Western blot was performed from the immunoprecipitate using ab241975 at 1/250 dilution.

Lane 1 : ab241975 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-DDHD1 antibody (ab241975)

Predicted band size: 100 kDa

false

Western blot - Anti-DDHD1 antibody (AB241975)
  • WB

Supplier Data

Western blot - Anti-DDHD1 antibody (AB241975)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-DDHD1 antibody (ab241975) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 100 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DDHD1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q8NEL9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 20 μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241975 was affinity purified using an epitope specific to DDHD1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDHD1 protein also known as DDH1 or PA-PLA1 is a phospholipase involved in lipid metabolism. With a molecular mass of approximately 88 kDa this protein localizes primarily in the cytosol but can also associate with the Golgi apparatus. DDHD1 is expressed in various tissues with significant levels in the brain and testis. This expression pattern suggests its potential role in central nervous system functions and reproductive processes.
Biological function summary

DDHD1 enzyme is responsible for the hydrolysis of phosphatidic acid contributing to lysophospholipid production. It is part of the intracellular membrane trafficking system operating independently rather than as part of a complex. By modulating the lipid composition of membranes DDHD1 helps regulate membrane curvature and dynamics which affects cellular processes such as vesicular transport and signaling.

Pathways

DDHD1 plays a significant role in the phospholipid signaling pathway by acting on phosphatidic acids. Its activity influences the levels of signaling molecules like lysophosphatidic acid which impact cellular events like proliferation and migration. Additionally DDHD1 is involved in the arachidonic acid pathway connecting it with enzymes such as PLA2 which also mediate the release of fatty acids from phospholipids essential for inflammatory responses.

DDHD1 mutations have been associated with hereditary spastic paraplegia a neurodegenerative condition characterized by progressive weakness and spasticity of the lower limbs. This relationship indicates its contribution to maintaining neuronal integrity. Furthermore DDHD1 function has links to mitochondrial lipid homeostasis associating it with DDHD2 in the context of complex lipid synthesis disorders especially those affecting the nervous system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phospholipase A1 (PLA1) that hydrolyzes ester bonds at the sn-1 position of glycerophospholipids producing a free fatty acid and a lysophospholipid (Probable) (PubMed : 20359546, PubMed : 22922100). Prefers phosphatidate (1,2-diacyl-sn-glycero-3-phosphate, PA) as substrate in vitro, but can efficiently hydrolyze phosphatidylinositol (1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol), PI), as well as a range of other glycerophospholipid substrates such as phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE), phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine, PS) and phosphatidylglycerol (1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol), PG) (Probable) (PubMed : 20359546). Involved in the regulation of the endogenous content of polyunsaturated PI and PS lipids in the nervous system. Changes in these lipids extend to downstream metabolic products like PI phosphates PIP and PIP2, which play fundamental roles in cell biology (By similarity). Regulates mitochondrial morphology (PubMed : 24599962). These dynamic changes may be due to PA hydrolysis at the mitochondrial surface (PubMed : 24599962). May play a regulatory role in spermatogenesis or sperm function (PubMed : 24599962).
See full target information DDHD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bone research 12:63 PubMed39465252

2024

IRF1-mediated upregulation of PARP12 promotes cartilage degradation by inhibiting PINK1/Parkin dependent mitophagy through ISG15 attenuating ubiquitylation and SUMOylation of MFN1/2.

Applications

Unspecified application

Species

Unspecified reactive species

Zengfa Deng,Dianbo Long,Changzhao Li,Hailong Liu,Wei Li,Yanlin Zhong,Xiaolin Mo,Ruiyun Li,Zibo Yang,Yan Kang,Guping Mao
View all publications
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Product promise

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