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AB235832

Anti-DDHD2 antibody

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(1 Publication)

Rabbit Polyclonal DDHD2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDHD2 aa 350-650.

View Alternative Names

KIAA0725, SAMWD1, DDHD2, Triacylglycerol hydrolase DDHD2, TAG hydrolase, DDHD domain-containing protein 2, KIAA0725p, Phospholipase DDHD2, Triglyceride hydrolase DDHD2, Triglyceride lipase

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD2 antibody (AB235832)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD2 antibody (AB235832)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling DDHD2 with ab235832 at 1/50 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DDHD2 aa 350-650. The exact immunogen used to generate this antibody is proprietary information.

O94830

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDHD2 also known as PA-PLA1 or KIAA0725 is a phospholipase enzyme with a molecular mass of approximately 90 kDa. This enzyme plays a role in lipid metabolism by hydrolyzing phosphatidic acid. DDHD2 is expressed mainly in the brain suggesting a specialized function in neural tissue. Its enzymatic activity contributes to the dynamic regulation of lipid composition within neuronal membranes influencing membrane fluidity and signaling cascades.
Biological function summary

DDHD2 serves as a critical player in neural lipid homeostasis. It exists as a part of a larger phospholipase complex that regulates various lipid signaling pathways in neurons. The enzyme's activity affects the production of lysophosphatidic acid and other bioactive lipids impacting processes like cell signaling growth and maintenance of neuronal membranes. Loss of DDHD2 activity can therefore disrupt lipid balance affecting neuronal function.

Pathways

DDHD2 actively participates in the phospholipase signaling pathway and the endocannabinoid signaling pathway. Through its interactions DDHD2 influences these pathways by modulating lipid mediators that serve as signaling molecules. It functions along with proteins such as lipin and phospholipase C in these pathways collectively impacting membrane lipid turnover and intracellular signaling events.

DDHD2 is linked with hereditary spastic paraplegia a condition characterized by progressive spasticity and weakness of the lower limbs. Mutations in DDHD2 can lead to defective lipid metabolism in neuronal cells contributing to the pathology of this disorder. Additionally DDHD2 is connected with proteins such as spastin and atlastin which are also implicated in hereditary spastic paraplegia sharing roles in maintaining neuron structure and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Diacylglycerol (DAG) and triacylglycerol (TAG) lipase required for proper lipid homeostasis in the central nervous system (PubMed : 29278326, PubMed : 37832604). It cooperates with PNPLA2/ATGL in neuronal TAG catabolism and hydrolyzes sn-1,3 DAG downstream of PNPLA2/ATGL (By similarity). In vitro, it also acts as a phospholipase that hydrolyzes preferentially phosphatidic acids, including 1,2-dioleoyl-sn-phosphatidic acid, phosphatidylcholine and phosphatidylethanolamine. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P), phosphatidylinositol 5-phosphate (PI(5)P) and possibly phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. May regulate the transport between Golgi apparatus and plasma membrane.
See full target information DDHD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:53 PubMed36709284

2023

Upregulation of HMGB1 in tumor-associated macrophages induced by tumor cell-derived lactate further promotes colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Gao,Shiqi Zhou,Zhaofu Qin,Dechuan Li,Yuping Zhu,Dening Ma
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Product promise

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