AB197081

Anti-DDI2 antibody

Name: Anti-DDI2 antibody (ab197081) | Abcam
Brand: Abcam Limited
SKU: AB197081
Price: 520 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal DDI2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human DDI2 aa 300 to C-terminus.

View Alternative Names

Protein DDI1 homolog 2, DDI2

2 Images

Supplier Data

Immunoprecipitation - Anti-DDI2 antibody (AB197081)

Detection of DDI2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197081 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DDI2 antibody (ab197081)

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-DDI2 antibody (AB197081)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-DDI2 antibody (ab197081) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 45 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DDI2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5TDH0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab197081 was affinity purified using an epitope specific to DDI2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDI2 also known as DNA-damage inducible 1 homolog 2 is a protein involved in several important cellular processes. It functions as an aspartic protease known for its capability to cleave ubiquitinated substrates. This protein has a molecular mass of approximately 50 kDa. Researchers often find DDI2 expression in various human tissues indicating its potential roles in diverse biological processes.

Biological function summary

DDI2 participates in the proteasome-mediated pathway a significant cellular mechanism that regulates protein degradation to maintain cellular homeostasis. It seems to play a part in the endoplasmic reticulum-associated degradation (ERAD) pathway where misfolded proteins get recognized and directed towards destruction. DDI2's involvement in these pathways suggests its critical function in protein quality control. It also may interact with other proteins that contribute to its protease activity enhancing its regulatory role within the cell.

Pathways

DDI2 functions in the context of the ubiquitin-proteasome system (UPS) a fundamental cellular pathway responsible for protein turnover. Through its protease activity DDI2 intersects with pathways involved in the degradation of damaged or misfolded proteins which connects it to the larger ERAD pathway. Evidence shows its interaction with proteins like p97/VCP which assists in protein degradation and ubiquitin which tags proteins for proteasomal activity highlighting DDI2's place in cellular quality control.

DDI2 is associated with several conditions involving protein homeostasis deficits. Its involvement in lipid metabolism disorders points to a potential role in conditions like Niemann-Pick disease. DDI2 dysfunction may result in the inadequate breakdown of proteins contributing to the pathological accumulation found in disorders related to lipid metabolism. Its interaction with the protein p97/VCP known to be involved in various degenerative diseases highlights the importance of understanding DDI2's mechanistic role in these pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane (PubMed : 27528193, PubMed : 27676298). Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1 (PubMed : 27528193). Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed : 29290612).

See full target information DDI2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110893 PubMed39328932

2024

DDI2 protease controls embryonic development and inflammation via TCF11/NRF1.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Nedomova,Stefanie Haberecht-Müller,Sophie Möller,Simone Venz,Michaela Prochazkova,Jan Prochazka,Frantisek Sedlak,Kallayanee Chawengsaksophak,Elke Hammer,Petr Kasparek,Michael Adamek,Radislav Sedlacek,Jan Konvalinka,Elke Krüger,Klara Grantz Saskova

Life science alliance 7: PubMed38803224

2024

K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Waltho,Oliver Popp,Christopher Lenz,Lukas Pluska,Mahil Lambert,Volker Dötsch,Philipp Mertins,Thomas Sommer

iScience 25:105227 PubMed36248746

2022

The protease DDI2 regulates NRF1 activation in response to cadmium toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Sérgio T Ribeiro,Aude de Gassart,Sarah Bettigole,Lea Zaffalon,Claire Chavarria,Melanie Op,Kalvin Nugraha,Fabio Martinon

Blood advances 6:429-440 PubMed34649278

2021

Multiple myeloma cells depend on the DDI2/NRF1-mediated proteasome stress response for survival.

Applications

Unspecified application

Species

Unspecified reactive species

Tianzeng Chen,Matthew Ho,Jenna Briere,Maria Moscvin,Peter G Czarnecki,Kenneth C Anderson,T Keith Blackwell,Giada Bianchi

Bioscience reports 38: PubMed30177525

2018

Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Karolin Nowak,Ramona M Taubert,Stefanie Haberecht,Simone Venz,Elke Krüger

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDI2 antibody

Immunoprecipitation - Anti-DDI2 antibody (AB197081)

Western blot - Anti-DDI2 antibody (AB197081)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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