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AB223034

Anti-DDIT4L/REDD2 antibody

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(2 Publications)

Rabbit Polyclonal DDIT4L/REDD2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDIT4L aa 1 to C-terminus.

View Alternative Names

REDD2, RTP801L, DDIT4L, DNA damage-inducible transcript 4-like protein, HIF-1 responsive protein RTP801-like, Protein regulated in development and DNA damage response 2, REDD-2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDIT4L/REDD2 antibody (AB223034)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDIT4L/REDD2 antibody (AB223034)

Paraffin-embedded human liver cancer tissue stained for DDIT4L/REDD2 using ab223034 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DDIT4L/REDD2 antibody (AB223034)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDIT4L/REDD2 antibody (AB223034)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DDIT4L/REDD2 (green) using ab223034 at 1/100 dilution in ICC/IF. Secondary : Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DDIT4L aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96D03

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDIT4L/REDD2 protein known alternatively as DDIT is a member of the DNA damage-inducible transcript family with a molecular mass of approximately 23 kDa. It is encoded by the DDIT4L gene and is expressed in multiple tissues with higher expression levels in muscle and fat tissues. This protein plays a role in cellular stress responses and has been associated with the regulation of cell growth and apoptosis.
Biological function summary

DDIT4L/REDD2 influences response to cellular stress signals and acts as an inhibitor of the mTOR pathway—an essential regulator of cell metabolism growth and proliferation. It does not function as part of a large complex but interacts with other proteins to mediate its effects. By inhibiting mTOR activity DDIT4L can affect protein synthesis and autophagy reflecting its role in maintaining cellular homeostasis under stress conditions.

Pathways

DDIT4L/REDD2 is intricately involved in the mTOR signaling cascade and influences the PI3K/AKT pathway two important pathways in cellular metabolism. Within these pathways it is related to proteins like TSC2 (Tuberous Sclerosis Complex 2) and AKT. DDIT4L exerts its effect by modulating signal transduction that impacts cell survival and stress responses making it an important player in the adaptation to environmental changes.

Alterations in DDIT4L/REDD2 expression are implicated in conditions such as cancer and metabolic syndromes. Aberrant regulation of this protein may lead to unchecked cell proliferation or metabolic imbalances involving proteins like mTOR and TSC2. These associations highlight the role of DDIT4L/REDD2 in the maintenance of normal cellular functions and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.
See full target information DDIT4L
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cells international 2022:9455152 PubMed36061148

2022

miR-3960 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Inactivates SDC1/Wnt/-Catenin Axis to Relieve Chondrocyte Injury in Osteoarthritis by Targeting PHLDA2.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Ye,Zhanhu Mi,Daihao Wei,Pengcheng Gao,Mei Ma,Haibo Yang

Computational and structural biotechnology journal 19:2775-2789 PubMed34093992

2021

Employing hypoxia characterization to predict tumour immune microenvironment, treatment sensitivity and prognosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fanhong Zeng,Yue Zhang,Xu Han,Min Zeng,Yi Gao,Jun Weng
View all publications
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Product promise

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