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AB151978

Anti-DDOST antibody

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Rabbit Polyclonal DDOST antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DDOST aa 200 to C-terminus.

View Alternative Names

KIAA0115, OST48, OK/SW-cl.45, DDOST, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, DDOST 48 kDa subunit, Oligosaccharyl transferase 48 kDa subunit

1 Images
Western blot - Anti-DDOST antibody (AB151978)
  • WB

Unknown

Western blot - Anti-DDOST antibody (AB151978)

10% SDS PAGE

All lanes:

Western blot - Anti-DDOST antibody (ab151978) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human DDOST aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P39656

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (DDOST) also known as oligosaccharyltransferase 48 kDa subunit weighs approximately 48 kDa. It is part of the oligosaccharyltransferase (OST) complex which is found mostly in the endoplasmic reticulum (ER). This protein functions to transfer glycan chains to nascent proteins during N-linked glycosylation an important post-translational modification. DDOST expresses widely across different tissues reflecting its importance in protein synthesis and folding.
Biological function summary

The DDOST protein plays a big role in the process of protein glycosylation. As part of the OST complex it facilitates the attachment of oligosaccharides to specific asparagine residues of target proteins in the ER. This glycosylation process helps with protein folding and stability and is important for the functionality and longevity of the proteins. Alterations in this system can affect protein structure and function illustrating DDOST's critical role in ensuring protein integrity and cellular homeostasis.

Pathways

DDOST participates rigorously in the N-glycosylation pathway. It interacts with key enzymes involved in the transfer of glycan moieties to nascent polypeptides which is necessary for proper cellular function. The protein also works closely with ribophorin and other subunits of the OST complex. While directly part of this glycosylation pathway its activity indirectly impacts various cellular signaling pathways due to its role in modifying and folding membrane-bound and secreted proteins.

Defects in DDOST relate to congenital disorders of glycosylation. Mutations in this protein can lead to improper glycosylation causing a range of symptoms from developmental delays to neurological issues. Also changes in glycosylation patterns have associations with cancer progression. Beyond DDOST itself glycosylation often affects other key proteins contributing to these conditions such as integrins and cadherins especially in the context of cell adhesion and signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes (PubMed : 22467853).
See full target information DDOST
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB204314

Anti-DDOST antibody

primary-antibodies

ddost-antibody-ab204314

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