Anti-DDOST antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (DDOST) also known as oligosaccharyltransferase 48 kDa subunit weighs approximately 48 kDa. It is part of the oligosaccharyltransferase (OST) complex which is found mostly in the endoplasmic reticulum (ER). This protein functions to transfer glycan chains to nascent proteins during N-linked glycosylation an important post-translational modification. DDOST expresses widely across different tissues reflecting its importance in protein synthesis and folding.

Biological function summary

The DDOST protein plays a big role in the process of protein glycosylation. As part of the OST complex it facilitates the attachment of oligosaccharides to specific asparagine residues of target proteins in the ER. This glycosylation process helps with protein folding and stability and is important for the functionality and longevity of the proteins. Alterations in this system can affect protein structure and function illustrating DDOST's critical role in ensuring protein integrity and cellular homeostasis.

Pathways

DDOST participates rigorously in the N-glycosylation pathway. It interacts with key enzymes involved in the transfer of glycan moieties to nascent polypeptides which is necessary for proper cellular function. The protein also works closely with ribophorin and other subunits of the OST complex. While directly part of this glycosylation pathway its activity indirectly impacts various cellular signaling pathways due to its role in modifying and folding membrane-bound and secreted proteins.

Associated diseases and disorders

Defects in DDOST relate to congenital disorders of glycosylation. Mutations in this protein can lead to improper glycosylation causing a range of symptoms from developmental delays to neurological issues. Also changes in glycosylation patterns have associations with cancer progression. Beyond DDOST itself glycosylation often affects other key proteins contributing to these conditions such as integrins and cadherins especially in the context of cell adhesion and signaling.