AB151978

Anti-DDOST antibody

Name: Anti-DDOST antibody (ab151978) | Abcam
Brand: Abcam Limited
SKU: AB151978
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DDOST antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDOST aa 200-500.

View Alternative Names

KIAA0115, OST48, OK/SW-cl.45, DDOST, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit, DDOST 48 kDa subunit, Oligosaccharyl transferase 48 kDa subunit

1 Images

Unknown

Western blot - Anti-DDOST antibody (AB151978)

10% SDS PAGE

All lanes:

Western blot - Anti-DDOST antibody (ab151978) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human DDOST aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

P39656

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (DDOST) also known as oligosaccharyltransferase 48 kDa subunit weighs approximately 48 kDa. It is part of the oligosaccharyltransferase (OST) complex which is found mostly in the endoplasmic reticulum (ER). This protein functions to transfer glycan chains to nascent proteins during N-linked glycosylation an important post-translational modification. DDOST expresses widely across different tissues reflecting its importance in protein synthesis and folding.

Biological function summary

The DDOST protein plays a big role in the process of protein glycosylation. As part of the OST complex it facilitates the attachment of oligosaccharides to specific asparagine residues of target proteins in the ER. This glycosylation process helps with protein folding and stability and is important for the functionality and longevity of the proteins. Alterations in this system can affect protein structure and function illustrating DDOST's critical role in ensuring protein integrity and cellular homeostasis.

Pathways

DDOST participates rigorously in the N-glycosylation pathway. It interacts with key enzymes involved in the transfer of glycan moieties to nascent polypeptides which is necessary for proper cellular function. The protein also works closely with ribophorin and other subunits of the OST complex. While directly part of this glycosylation pathway its activity indirectly impacts various cellular signaling pathways due to its role in modifying and folding membrane-bound and secreted proteins.

Defects in DDOST relate to congenital disorders of glycosylation. Mutations in this protein can lead to improper glycosylation causing a range of symptoms from developmental delays to neurological issues. Also changes in glycosylation patterns have associations with cancer progression. Beyond DDOST itself glycosylation often affects other key proteins contributing to these conditions such as integrins and cadherins especially in the context of cell adhesion and signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed : 31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes (PubMed : 22467853).

See full target information DDOST

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 14:e1007532 PubMed30102696

2018

A genome-wide association study identifies a susceptibility locus for biliary atresia on 2p16.1 within the gene EFEMP1.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Melissa A Gilbert,Christopher M Grochowski,Deborah McEldrew,Jessica Llewellyn,Orith Waisbourd-Zinman,Hakon Hakonarson,Joan E Bailey-Wilson,Pierre Russo,Rebecca G Wells,Kathleen M Loomes,Nancy B Spinner,Marcella Devoto

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDOST antibody

Western blot - Anti-DDOST antibody (AB151978)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise