JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB322921

Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33]

  • Recombinant
  • KO Validated
  • 20ul selling size
  • RabMAb
  • What is this?

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CD167a/DDR1 phospho Y796 antibody. Suitable for Dot, IP, WB and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK, MCK-10

4 Images
Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)
  • WB

Supplier Data

Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)

Blocking and diluting buffer and concentration : 1% BSA/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (ab322921) at 1/1000 dilution

Lane 1:

Untreated T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate (untreated membrane) at 10 µg

Lane 2:

T-47D treated with 20 μg/ml Collagen I for 18 hours whole cell lysate (untreated membrane) at 10 µg

Lane 3:

T-47D treated with 20 μg/ml Collagen I for 18 hours whole cell lysate (alkaline phosphatase treated membrane) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa,125 kDa

false

Exposure time: 37s

Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)
  • WB

Supplier Data

Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)

Blocking and diluting buffer and concentration : 1% BSA/TBST.

The band beneath the target band around 60KD is likely to be degraded target fragments.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (ab322921) at 1/1000 dilution

Lane 1:

Untreated DDR1 KO MCF7(FR200431) whole cell lysate (untreated membrane) at 10 µg

Lane 2:

DDR1 KO MCF7(FR200431) treated with 100uM pervanadate for 10 minutes whole cell lysate (untreated membrane) at 10 µg

Lane 3:

Untreated MCF7 whole cell lysate 10 &mu;g (untreated membrane) at 10 µg

Lane 4:

MCF7 treated with 100uM pervanadate for 10 minutes whole cell lysate (untreated membrane) at 10 µg

Lane 5:

Untreated DDR1 KO MCF7(FR200431) whole cell lysate (alkaline phosphatase treated membrane) at 10 µg

Lane 6:

DDR1 KO MCF7(FR200431) treated with 100uM pervanadate for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 10 µg

Lane 7:

Untreated MCF7 whole cell lysate (alkaline phosphatase treated membrane) at 10 µg

Lane 8:

MCF7 treated with 100uM pervanadate for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 36 kDa,125 kDa

false

Exposure time: 103s

Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)
  • IP

Supplier Data

Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)

DDR1 (phospho Y796) + DDR2 (phospho Y740) was immunoprecipitated from 0.35 mg T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate with ab322921 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322921 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (ab322921) at 1/1000 dilution

Lane 1:

T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg

Lane 2:

ab322921 at 1/30 IP in T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab322921 in T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Observed band size: 125 kDa

false

Exposure time: 76s

Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)
  • Dot

Supplier Data

Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (AB322921)

Dot blot analysis of DDR1 (phospho Y796) + DDR2 (phospho Y740) using ab322921 at 1 : 1000 (0.513 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : Human DDR1 (pTyr796) recombinant fragment
Lane2 : Human DDR2 (pY740) recombinant fragment
Lane3 : Human DDR1 recombinant fragment
Lane4 : Human DDR2 recombinant fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody recognises DDR1(pTyr796) and DDR2 (pY740) because of high sequence homology.

All lanes:

Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (ab322921) at 1/1000 dilution

Lane 1:

Human DDR1 (pTyr796) recombinant fragment

Lane 2:

Human DDR2 (pY740) recombinant fragment

Lane 3:

Human DDR1 recombinant fragment

Lane 4:

Human DDR2 recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

  • Carrier free

    Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28699-33

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
See full target information DDR1 phospho Y796

Additional targets

DDR2 phospho Y740
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com