Rabbit Recombinant Monoclonal CD167a/DDR1 phospho Y796 antibody. Carrier free. Suitable for Dot, IP, WB and reacts with Recombinant fragment - Human, Human samples.
Images
![Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--dot-blot-img444681.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745)")
![Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--immunoprecipitation-img444694.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745)")
![Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--western-blot-img444810.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745)")
![Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--western-blot-img444819.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (AB323745)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Recombinant fragment - Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Additional Targets
DDR2 phospho Y740
Alternative names
CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK, MCK-10
Rabbit Recombinant Monoclonal CD167a/DDR1 phospho Y796 antibody. Carrier free. Suitable for Dot, IP, WB and reacts with Recombinant fragment - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28699-33
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab323745 is the carrier free version of Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--dot-blot-img444681.jpg "Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)")
Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)
All lanes: Dot Blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921) at 1/1000 dilution
Lane 1: Human DDR1 (pTyr796) recombinant fragment
Lane 2: Human DDR2 (pY740) recombinant fragment
Lane 3: Human DDR1 recombinant fragment
Lane 4: Human DDR2 recombinant fragment
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--immunoprecipitation-img444694.jpg "Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)")
Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)
This data was developed using Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921, the same antibody clone in a different buffer formulation
DDR1 (phospho Y796) + DDR2 (phospho Y740) was immunoprecipitated from 0.35 mg T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate with Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921 at 1/1000 dilution.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921) at 1/1000 dilution
Lane 1: T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg
Lane 2: Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921 at 1/30 IP in T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921 in T-47D treated with 20 ug/ml Collagen I for 18 hours whole cell lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/5000 dilution
Observed band size: 125 kDa
Exposure time: 76s
![Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--western-blot-img444810.jpg "Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)")
Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)
This data was developed using Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921, the same antibody clone in a different buffer formulation
Blocking and diluting buffer and concentration: 1% BSA/TBST.
The band beneath the target band around 60KD is likely to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921) at 1/1000 dilution
Lane 1: Untreated DDR1 KO MCF7(FR200431) whole cell lysate (untreated membrane) at 10 µg
Lane 2: DDR1 KO MCF7(FR200431) treated with 100uM pervanadate for 10 minutes whole cell lysate (untreated membrane) at 10 µg
Lane 3: Untreated MCF7 whole cell lysate 10 μg (untreated membrane) at 10 µg
Lane 4: MCF7 treated with 100uM pervanadate for 10 minutes whole cell lysate (untreated membrane) at 10 µg
Lane 5: Untreated DDR1 KO MCF7(FR200431) whole cell lysate (alkaline phosphatase treated membrane) at 10 µg
Lane 6: DDR1 KO MCF7(FR200431) treated with 100uM pervanadate for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 10 µg
Lane 7: Untreated MCF7 whole cell lysate (alkaline phosphatase treated membrane) at 10 µg
Lane 8: MCF7 treated with 100uM pervanadate for 10 minutes whole cell lysate (alkaline phosphatase treated membrane) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 125 kDa, 36 kDa
Exposure time: 103s
![Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745), expandable thumbnail](https://content.abcam.com/products/images/ddr1-phospho-y796-ddr2-phospho-y740-antibody-epr28699-33-bsa-and-azide-free-ab323745--western-blot-img444819.jpg "Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)")
Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] - BSA and Azide free (ab323745)
This data was developed using Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921, the same antibody clone in a different buffer formulation
Blocking and diluting buffer and concentration: 1% BSA/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] (Anti-DDR1 (phospho Y796) + DDR2 (phospho Y740) antibody [EPR28699-33] ab322921) at 1/1000 dilution
Lane 1: Untreated T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate (untreated membrane) at 10 µg
Lane 2: T-47D treated with 20 μg/ml Collagen I for 18 hours whole cell lysate (untreated membrane) at 10 µg
Lane 3: T-47D treated with 20 μg/ml Collagen I for 18 hours whole cell lysate (alkaline phosphatase treated membrane) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 125 kDa, 36 kDa
Exposure time: 37s
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