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AB63337

Anti-DDR2 antibody [3B11E4]

4

(2 Reviews)

|

(8 Publications)

Mouse Monoclonal DDR2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Recombinant fragment - Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDR2.

View Alternative Names

CD167b, NTRKR3, TKT, TYRO10, DDR2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody [3B11E4] (AB63337)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody [3B11E4] (AB63337)

ab63337 at 1/1000 dilution staining DDR2 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections).

Immunocytochemistry/ Immunofluorescence - Anti-DDR2 antibody [3B11E4] (AB63337)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDR2 antibody [3B11E4] (AB63337)

ab63337 at 1/1000 dilution staining DDR2 in human A549 cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® conjugated Goat anti-mouse was used as secondary. The actin filaments were labeled red with DY-554 phalloidin whilst nuclei were stained blue with DRAQ5 fluorescent DNA dye.

Flow Cytometry - Anti-DDR2 antibody [3B11E4] (AB63337)
  • Flow Cyt

Lab

Flow Cytometry - Anti-DDR2 antibody [3B11E4] (AB63337)

Overlay histogram showing A549 cells stained with ab63337 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63337, 1/200 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a (ab170191, 0.5μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-DDR2 antibody [3B11E4] (AB63337)
  • WB

Unknown

Western blot - Anti-DDR2 antibody [3B11E4] (AB63337)

All lanes:

Western blot - Anti-DDR2 antibody [3B11E4] (ab63337) at 1/500 dilution

Lane 1:

molecular weight markers

Lane 2:

truncated recombinant DDR2 (immunizing peptide)

Predicted band size: 97 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B11E4

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DDR2. The exact immunogen used to generate this antibody is proprietary information.

Q16832

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/200", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/2000", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Discoidin Domain Receptor 2 (DDR2) also known as Tyrosine-protein kinase DDR2 is a receptor tyrosine kinase that binds to collagen initiating various cellular processes. It has a molecular mass of approximately 97 kDa. This receptor is expressed in various tissues including skin bone and cartilage as well as in certain cancer cells. DDR2 activation is achieved through the recognition of specific motifs such as GFOGER in collagen leading to signaling cascades that impact cell functions like adhesion proliferation and extracellular matrix remodeling.
Biological function summary

DDR2 functions as an integral part of the cellular response to collagen. The receptor plays a critical role in the development and maintenance of connective tissues. It exists as a monomer but can form complexes upon ligand binding facilitating its activity. DDR2 signaling influences critical processes such as wound healing and tissue homeostasis by modulating matrix metalloproteinases (MMPs) activity which are important for remodeling the extracellular matrix.

Pathways

DDR2 abettors collagen-induced signal transduction particularly within the mitogen-activated protein kinase (MAPK) pathway and the Src family kinase pathway. These pathways involve proteins like SHP2 and Src both of which interact with DDR2 to propagate cellular signals. DDR2 activation leads to phosphorylation events that stimulate cytoskeletal rearrangements and gene expression modulations relevant to cellular growth and differentiation.

DDR2 is linked to fibrosis and cancer. The receptor's overexpression or aberrant signaling can contribute to fibrotic diseases characterized by excessive scarring by promoting collagen deposition and fibroblast proliferation. In cancer DDR2 dysregulation often associates with tumor progression and metastasis particularly in lung cancer and sarcomas. This links DDR2 with other oncogenic proteins and pathways including those governed by MMP proteins which are involved in tumor invasion and metastasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine kinase involved in the regulation of tissues remodeling (PubMed : 30449416). It functions as a cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
See full target information DDR2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1496884 PubMed39698493

2024

Molecular and metabolomic characterization of hiPSC-derived cardiac fibroblasts transitioning to myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Raghu Sundaresan Nagalingam,Farah Jayousi,Homa Hamledari,Saif Dababneh,Dina Hosseini,Chloe Lindsay,Ramon Klein Geltink,Philipp F Lange,Ian Michael Dixon,Robert Alan Rose,Michael Paul Czubryt,Glen Findlay Tibbits

iScience 27:109585 PubMed38623327

2024

Craniofacial chondrogenesis in organoids from human stem cell-derived neural crest cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Foltz,Nagashree Avabhrath,Jean-Marc Lanchy,Tyler Levy,Anthony Possemato,Majd Ariss,Bradley Peterson,Mark Grimes

Quantitative imaging in medicine and surgery 11:3518-3534 PubMed34341728

2021

Tumor elastography and its association with cell-free tumor DNA in the plasma of breast tumor patients: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Hao,Wei Yang,Wenyi Zheng,Xiaona Chen,Hui Wang,Liang Zhao,Jinfeng Xu,Xia Guo

Stem cell research & therapy 10:316 PubMed31685023

2019

Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Seth Mount,Pushpinder Kanda,Sandrine Parent,Saad Khan,Connor Michie,Liliana Davila,Vincent Chan,Ross A Davies,Haissam Haddad,David Courtman,Duncan J Stewart,Darryl R Davis

Cell calcium 84:102099 PubMed31614270

2019

Oxytocin induces intracellular Ca release in cardiac fibroblasts from neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ifrah I Ali,Suhail Al-Salam,Frank C Howarth,Anatoliy Shmygol

Cancer research 78:1169-1183 PubMed29311158

2018

Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated β-Catenin Stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Xiaojing Wang,Hong Mei,Erhu Fang,Lin Ye,Huajie Song,Feng Yang,Huanhuan Li,Kai Huang,Liduan Zheng,Qiangsong Tong

Heliyon 3:e00406 PubMed29022008

2017

Inflammatory activation of human cardiac fibroblasts leads to altered calcium signaling, decreased connexin 43 expression and increased glutamate secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Skiöldebrand,Annika Lundqvist,Ulrika Björklund,Mikael Sandstedt,Anders Lindahl,Elisabeth Hansson,Lillemor Mattsson Hultén

International journal of clinical and experimental 8:5658-65 PubMed26191278

2015

Evaluation of correlation of articular cartilage staining for DDR2 and proteoglycans with histological tissue damage and the results of radiographic assessment in patients with early stages of knee osteoarthritis.

Applications

IHC

Species

Unspecified reactive species

Siim Suutre,Irina Kerna,Mare Lintrop,Hannes Tamm,Marina Aunapuu,Andres Arend,Agu Tamm
View all publications
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