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AB203219

Anti-DDR2 antibody

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(4 Publications)

Rabbit Polyclonal DDR2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DDR2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD167b, NTRKR3, TKT, TYRO10, DDR2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (AB203219)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDR2 antibody (AB203219)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue, labeling DDR2 with ab203219 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human DDR2 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q16832

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Discoidin Domain Receptor 2 (DDR2) also known as Tyrosine-protein kinase DDR2 is a receptor tyrosine kinase that binds to collagen initiating various cellular processes. It has a molecular mass of approximately 97 kDa. This receptor is expressed in various tissues including skin bone and cartilage as well as in certain cancer cells. DDR2 activation is achieved through the recognition of specific motifs such as GFOGER in collagen leading to signaling cascades that impact cell functions like adhesion proliferation and extracellular matrix remodeling.
Biological function summary

DDR2 functions as an integral part of the cellular response to collagen. The receptor plays a critical role in the development and maintenance of connective tissues. It exists as a monomer but can form complexes upon ligand binding facilitating its activity. DDR2 signaling influences critical processes such as wound healing and tissue homeostasis by modulating matrix metalloproteinases (MMPs) activity which are important for remodeling the extracellular matrix.

Pathways

DDR2 abettors collagen-induced signal transduction particularly within the mitogen-activated protein kinase (MAPK) pathway and the Src family kinase pathway. These pathways involve proteins like SHP2 and Src both of which interact with DDR2 to propagate cellular signals. DDR2 activation leads to phosphorylation events that stimulate cytoskeletal rearrangements and gene expression modulations relevant to cellular growth and differentiation.

DDR2 is linked to fibrosis and cancer. The receptor's overexpression or aberrant signaling can contribute to fibrotic diseases characterized by excessive scarring by promoting collagen deposition and fibroblast proliferation. In cancer DDR2 dysregulation often associates with tumor progression and metastasis particularly in lung cancer and sarcomas. This links DDR2 with other oncogenic proteins and pathways including those governed by MMP proteins which are involved in tumor invasion and metastasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine kinase involved in the regulation of tissues remodeling (PubMed : 30449416). It functions as a cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
See full target information DDR2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 8:1178 PubMed33241027

2020

Delay in articular cartilage degeneration of the knee joint by the conditional removal of discoidin domain receptor 2 in a spontaneous mouse model of osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Li,Yi Chen,Rui Xu,Yan Wang,Fan Jian,Hu Long,Wenli Lai

The Journal of pharmacology and experimental therapeutics 376:148-160 PubMed33168642

2020

Transport Properties of Statins by Organic Anion Transporting Polypeptide 1A2 and Regulation by Transforming Growth Factor- Signaling in Human Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick T Ronaldson,Hrvoje Brzica,Wazir Abdullahi,Bianca G Reilly,Thomas P Davis

Arthritis & rheumatology (Hoboken, N.J.) 72:943-956 PubMed32362074

2020

Blockade of Discoidin Domain Receptor 2 as a Strategy for Reducing Inflammation and Joint Destruction in Rheumatoid Arthritis Via Altered Interleukin-15 and Dkk-1 Signaling in Fibroblast-Like Synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Mu,Jin-Tao Gu,Tong-Lie Huang,Nan-Nan Liu,Hui Chen,Xin Bu,Zhao-Hui Zheng,Bo Jia,Jun Liu,Bao-Long Wang,Ying-Mei Wang,Zhen-Feng Zhu,Yong Zhang,Ying-Qi Zhang,Xiao-Chang Xue,Meng Li,Wei Zhang

The American journal of pathology 189:1423-1434 PubMed31051168

2019

High-Temperature Requirement A1 Protease as a Rate-Limiting Factor in the Development of Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Peter H Chen,Tian Tang,Chenlu Liu,Beiyu Wang,Michelle Mian,Chio Oka,Maria Baquerizo,Yefu Li,Lin Xu
View all publications
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