JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB280369

Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal DDR2 antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples.

View Alternative Names

CD167b, NTRKR3, TKT, TYRO10, DDR2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10

5 Images
Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)
  • WB

Lab

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)

This data was developed using ab280354, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

The expression pattern is consistent with what has been described in the literature (PMID : 15111304, 29616590).

Negative control : MDA-MB-231 (PMID : 28147276, 29616590)

Exposure time : 158 seconds.

All lanes:

Western blot - Anti-DDR2 antibody [EPR18826-119] (<a href='/en-us/products/primary-antibodies/ddr2-antibody-epr18826-119-ab280354'>ab280354</a>) at 1/1000 dilution

Lane 1:

HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 3:

A-204 (human muscle rhabdomyosarcoma) whole cell lysate at 20 µg

Lane 4:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 120-130 kDa,70 kDa

false

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)
  • WB

Lab

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)

This data was developed using ab280354, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression pattern is consistent with what has been described in the literature (PMID : 15111304, 29616590).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-DDR2 antibody [EPR18826-119] (<a href='/en-us/products/primary-antibodies/ddr2-antibody-epr18826-119-ab280354'>ab280354</a>) at 1/1000 dilution

Lane 1:

Mouse ovary tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 120-130 kDa,70 kDa

false

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)
  • WB

Lab

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)

This data was developed using ab280354, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody can not recognize human DDR1.

Both recombinant proteins are made in house and extracted from HEK-293 expression systems.

Exposure time : 8 seconds.

All lanes:

Western blot - Anti-DDR2 antibody [EPR18826-119] (<a href='/en-us/products/primary-antibodies/ddr2-antibody-epr18826-119-ab280354'>ab280354</a>) at 1/1000 dilution

Lane 1:

His-tagged human DDR2 recombinant protein (aa22-399) at 0.03 µg

Lane 2:

His-tagged human DDR1 recombinant protein (aa21-416) at 0.03 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 60 kDa

false

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)
  • WB

Lab

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)

This data was developed using ab280354, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression pattern is consistent with what has been described in the literature (PMID : 15111304, 29616590).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-DDR2 antibody [EPR18826-119] (<a href='/en-us/products/primary-antibodies/ddr2-antibody-epr18826-119-ab280354'>ab280354</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

Rat ovary tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 97 kDa

Observed band size: 120-130 kDa,70 kDa

false

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)
  • WB

Lab

Western blot - Anti-DDR2 antibody [EPR18826-119] - BSA and Azide free (AB280369)

This data was developed using ab280354, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression pattern is consistent with what has been described in the literature (PMID : 15111304, 29616590).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-DDR2 antibody [EPR18826-119] (<a href='/en-us/products/primary-antibodies/ddr2-antibody-epr18826-119-ab280354'>ab280354</a>) at 1/1000 dilution

Lane 1:

Human stomach tissue lysate at 20 µg

Lane 2:

Human ovary tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 97 kDa

Observed band size: 120-130 kDa,70 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18826-119

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab280369 is the carrier-free version of ab280354.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Discoidin Domain Receptor 2 (DDR2) also known as Tyrosine-protein kinase DDR2 is a receptor tyrosine kinase that binds to collagen initiating various cellular processes. It has a molecular mass of approximately 97 kDa. This receptor is expressed in various tissues including skin bone and cartilage as well as in certain cancer cells. DDR2 activation is achieved through the recognition of specific motifs such as GFOGER in collagen leading to signaling cascades that impact cell functions like adhesion proliferation and extracellular matrix remodeling.
Biological function summary

DDR2 functions as an integral part of the cellular response to collagen. The receptor plays a critical role in the development and maintenance of connective tissues. It exists as a monomer but can form complexes upon ligand binding facilitating its activity. DDR2 signaling influences critical processes such as wound healing and tissue homeostasis by modulating matrix metalloproteinases (MMPs) activity which are important for remodeling the extracellular matrix.

Pathways

DDR2 abettors collagen-induced signal transduction particularly within the mitogen-activated protein kinase (MAPK) pathway and the Src family kinase pathway. These pathways involve proteins like SHP2 and Src both of which interact with DDR2 to propagate cellular signals. DDR2 activation leads to phosphorylation events that stimulate cytoskeletal rearrangements and gene expression modulations relevant to cellular growth and differentiation.

DDR2 is linked to fibrosis and cancer. The receptor's overexpression or aberrant signaling can contribute to fibrotic diseases characterized by excessive scarring by promoting collagen deposition and fibroblast proliferation. In cancer DDR2 dysregulation often associates with tumor progression and metastasis particularly in lung cancer and sarcomas. This links DDR2 with other oncogenic proteins and pathways including those governed by MMP proteins which are involved in tumor invasion and metastasis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tyrosine kinase involved in the regulation of tissues remodeling (PubMed : 30449416). It functions as a cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
See full target information DDR2
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB150376

Anti-LRRC15 antibody [EPR8188(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)

  • 4
  • 4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com