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AB313830

Anti-DDR2 antibody [HL1107] - BSA and Azide free

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Rabbit Recombinant Monoclonal DDR2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human DDR2.

View Alternative Names

CD167b, NTRKR3, TKT, TYRO10, DDR2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, CD167 antigen-like family member B, Discoidin domain-containing receptor tyrosine kinase 2, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10, CD167b, Ntrkr3, Tkt, Tyro10, Ddr2, Discoidin domain-containing receptor 2, Discoidin domain receptor 2, CD167 antigen-like family member B, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10

4 Images
Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)
  • WB

Supplier Data

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (ab313830) at 1/500000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 96 kDa

false

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)
  • WB

Supplier Data

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (ab313830) at 1/10000 dilution

Lane 1:

U87-MG Whole cell extract at 5 µg

Lane 2:

U87-MG membrane extract at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 96 kDa

false

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)
  • WB

Supplier Data

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (ab313830) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 96 kDa

false

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)
  • WB

Supplier Data

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (AB313830)

All lanes:

Western blot - Anti-DDR2 antibody [HL1107] - BSA and Azide free (ab313830) at 1/1000 dilution

Lane 1:

Mouse heart tissue extract at 50 µg

Lane 2:

Rat heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1107

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human DDR2.

Q16832

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Discoidin Domain Receptor 2 (DDR2) also known as Tyrosine-protein kinase DDR2 is a receptor tyrosine kinase that binds to collagen initiating various cellular processes. It has a molecular mass of approximately 97 kDa. This receptor is expressed in various tissues including skin bone and cartilage as well as in certain cancer cells. DDR2 activation is achieved through the recognition of specific motifs such as GFOGER in collagen leading to signaling cascades that impact cell functions like adhesion proliferation and extracellular matrix remodeling.
Biological function summary

DDR2 functions as an integral part of the cellular response to collagen. The receptor plays a critical role in the development and maintenance of connective tissues. It exists as a monomer but can form complexes upon ligand binding facilitating its activity. DDR2 signaling influences critical processes such as wound healing and tissue homeostasis by modulating matrix metalloproteinases (MMPs) activity which are important for remodeling the extracellular matrix.

Pathways

DDR2 abettors collagen-induced signal transduction particularly within the mitogen-activated protein kinase (MAPK) pathway and the Src family kinase pathway. These pathways involve proteins like SHP2 and Src both of which interact with DDR2 to propagate cellular signals. DDR2 activation leads to phosphorylation events that stimulate cytoskeletal rearrangements and gene expression modulations relevant to cellular growth and differentiation.

DDR2 is linked to fibrosis and cancer. The receptor's overexpression or aberrant signaling can contribute to fibrotic diseases characterized by excessive scarring by promoting collagen deposition and fibroblast proliferation. In cancer DDR2 dysregulation often associates with tumor progression and metastasis particularly in lung cancer and sarcomas. This links DDR2 with other oncogenic proteins and pathways including those governed by MMP proteins which are involved in tumor invasion and metastasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine kinase involved in the regulation of tissues remodeling (PubMed : 30449416). It functions as a cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
See full target information DDR2

Additional targets

Ddr2,Ddr2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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