Rabbit Polyclonal DDT antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDT.
Images
Publications
Filter by
- EBioMedicine 74:1037142021Proteomic profiling identifies signatures associated with progression of precancerous gastric lesions and risk of early gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Li,Nai-Ren Zheng,Lin-Heng Wang,Zhong-Wu Li,Zong-Chao Liu,Hua Fan,Yi Wang,Jin Dai,Xiao-Tian Ni,Xin Wei,Ming-Wei Liu,Kai Li,Zhe-Xuan Li,Tong Zhou,Yang Zhang,Jing-Ying Zhang,Gaohaer Kadeerhan,Sha Huang,Wen-Hui Wu,Wei-Dong Liu,Xiu-Zhen Wu,Lan-Fu Zhang,Jian-Ming Xu,Markus Gerhard,Wei-Cheng You,Kai-Feng Pan,Wen-Qing Li,Jun QinPubMed 34818622
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DDT. The exact immunogen used to generate this antibody is proprietary information. Database link P30046
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Species Mouse | Dilution info 0.10000-0.50000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
Alternative names
D-dopachrome decarboxylase, D-dopachrome tautomerase, Phenylpyruvate tautomerase II, DDT
Recommended products
Rabbit Polyclonal DDT antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDT.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DDT. The exact immunogen used to generate this antibody is proprietary information. Database link P30046
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DDT also referred to as D-dopachrome tautomerase is an important protein involved in several biological processes. It has a molecular mass of approximately 12 kDa. The protein is widely expressed in multiple tissues particularly in the liver and macrophages which are integral to immune response and metabolism. Analyzing DDT expression through Western blot techniques can provide insights into its concentration and functional state in various tissues.
- Biological function summary
D-dopachrome tautomerase plays roles in melanin biosynthesis and inflammatory processes. It forms part of a complex with the macrophage migration inhibitory factor (MIF) influencing immune response regulation. DDT shares structural and functional similarities with MIF suggesting evolutionary links between these proteins and indicating possible redundant or cooperative roles in immune system modulation.
- Pathways
DDT is involved in the immune response pathway and the melanin synthesis pathway. Within the immune response its interactions have connections with cytokine regulation and leukocyte movement. DDT's interaction with MIF further highlights its involvement in modulating cytokine activity. In melanin synthesis DDT contributes to the conversion processes within the pathway although proteins like tyrosinase also play significant roles.
- Associated diseases and disorders
Links exist between DDT and inflammatory diseases like rheumatoid arthritis and skin disorders like melanoma. Higher DDT levels can coincide with increased inflammation seen in rheumatoid arthritis where it potentially works alongside MIF to modulate immune activity. In melanoma DDT's role in melanin synthesis may connect it to abnormal pigment cell growth and tumor progression. Understanding DDT's interactions with other proteins in these contexts could lead to better diagnostic or therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDT antibody (ab115785)
DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab115785 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex DAB as the chromogen.
Western blot - Anti-DDT antibody (ab115785)
All lanes: Western blot - Anti-DDT antibody (ab115785) at 0.5 µg/mL
Lane 1: rat liver tissues lysate at 50 µg
Lane 2: rat testis tissues lysate at 50 µg
Lanes 3 - 4: mouse liver tissues lysate at 50 µg
SecondaryAll lanes: goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 13 kDa
Western blot - Anti-DDT antibody (ab115785)
All lanes: Western blot - Anti-DDT antibody (ab115785) at 1 µg/mL
Lane 1: Rat brain tissue lysate
Lane 2: Rat pancreas tissue lysate
Lane 3: Rat liver tissue lysate
Lane 4: Rat lung tissue lysate
Predicted band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDT antibody (ab115785)
ab115785 at 1μg/ml staining DTT in Paraffin-embedded Human placenta tissue by Immunohistochemistry.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com