Rabbit Polyclonal DDX1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DDX1 aa 250-650.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DDX1 aa 250-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q92499
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed:24870230). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (By similarity). (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs. (Microbial infection) Required for Coronavirus IBV replication.
Alternative names
ATP-dependent RNA helicase DDX1, DEAD box protein 1, DEAD box protein retinoblastoma, DBP-RB, DDX1
Recommended products
Rabbit Polyclonal DDX1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DDX1 aa 250-650.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DDX1 aa 250-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q92499
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein DDX1 also known as DEAD-box helicase 1 is an RNA helicase with a mass of approximately 82 kDa. This protein facilitates the unwinding of RNA or RNA-DNA duplexes which is essential for various RNA metabolic processes. DDX1 possesses ATPase activity that fuels its helicase function enabling it to remodel RNA secondary structures. The protein is expressed in multiple tissues with significant expression observed in the nucleus and cytoplasm of cells.
- Biological function summary
DDX1 plays a role in RNA processing and ribonucleoprotein complex formation. It participates in pre-mRNA splicing and ribosomal RNA processing contributing to efficient macromolecular synthesis. DDX1 associates with the Drosha-DGCR8 complex playing a role in microRNA biogenesis which is important for regulating gene expression. By aiding these processes DDX1 supports cellular growth and division which are fundamental to normal cellular function.
- Pathways
DDX1 is involved in both RNA transport and processing pathways. In the RNA transport pathway it interacts with other proteins like Exportin-1 to mediate the translocation of RNA molecules between the nucleus and the cytoplasm. Within the RNA processing pathways DDX1 functions alongside proteins such as DDX5 to modulate splicing events that are vital for generating mature messenger RNAs. These pathways highlight DDX1's central role in managing RNA fate within the cell.
- Associated diseases and disorders
DDX1 has associations with certain cancers and neurological disorders. In particular its dysregulation is linked to neuroblastoma where altered DDX1 expression might affect cell proliferation. Additionally DDX1 interacts with BRCA1 during DNA repair processes implicating its potential involvement in breast cancer. Understanding DDX1's role in these conditions can help in exploring therapeutic strategies targeting its pathway interactions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-DDX1 antibody (ab151962)
7.5% SDS PAGE
All lanes: Western blot - Anti-DDX1 antibody (ab151962) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Predicted band size: 82 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DDX1 antibody (ab151962)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling DDX1 with ab151962 at 1/200. Image at the bottom costained with Hoechst 33342.
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