AB151962

Anti-DDX1 antibody

Name: Anti-DDX1 antibody (ab151962) | Abcam
Brand: Abcam Limited
SKU: AB151962
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DDX1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX1 aa 250-650.

View Alternative Names

ATP-dependent RNA helicase DDX1, DEAD box protein 1, DEAD box protein retinoblastoma, DBP-RB, DDX1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB151962)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB151962)

Immunohistochemical analysis of paraffin embedded rat fore brain tissue stained for DDX1 protein at nucleus with ab151962 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB151962)

Immunohistochemical analysis of paraffin embedded mouse fore brain tissue stained for DDX1 protein at nucleus with ab151962 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supplier Data

Western blot - Anti-DDX1 antibody (AB151962)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-DDX1 antibody (ab151962) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 82 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DDX1 aa 250-650. The exact immunogen used to generate this antibody is proprietary information.

Q92499

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein DDX1 also known as DEAD-box helicase 1 is an RNA helicase with a mass of approximately 82 kDa. This protein facilitates the unwinding of RNA or RNA-DNA duplexes which is essential for various RNA metabolic processes. DDX1 possesses ATPase activity that fuels its helicase function enabling it to remodel RNA secondary structures. The protein is expressed in multiple tissues with significant expression observed in the nucleus and cytoplasm of cells.

Biological function summary

DDX1 plays a role in RNA processing and ribonucleoprotein complex formation. It participates in pre-mRNA splicing and ribosomal RNA processing contributing to efficient macromolecular synthesis. DDX1 associates with the Drosha-DGCR8 complex playing a role in microRNA biogenesis which is important for regulating gene expression. By aiding these processes DDX1 supports cellular growth and division which are fundamental to normal cellular function.

Pathways

DDX1 is involved in both RNA transport and processing pathways. In the RNA transport pathway it interacts with other proteins like Exportin-1 to mediate the translocation of RNA molecules between the nucleus and the cytoplasm. Within the RNA processing pathways DDX1 functions alongside proteins such as DDX5 to modulate splicing events that are vital for generating mature messenger RNAs. These pathways highlight DDX1's central role in managing RNA fate within the cell.

DDX1 has associations with certain cancers and neurological disorders. In particular its dysregulation is linked to neuroblastoma where altered DDX1 expression might affect cell proliferation. Additionally DDX1 interacts with BRCA1 during DNA repair processes implicating its potential involvement in breast cancer. Understanding DDX1's role in these conditions can help in exploring therapeutic strategies targeting its pathway interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB : together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed : 24870230). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I : C) dsRNA (By similarity).. (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs.. (Microbial infection) Required for Coronavirus IBV replication.

See full target information DDX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 7: PubMed29345618

2018

NECAPs are negative regulators of the AP2 clathrin adaptor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Gwendolyn M Beacham,Edward A Partlow,Jeffrey J Lange,Gunther Hollopeter

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDX1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB151962)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB151962)

Western blot - Anti-DDX1 antibody (AB151962)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise