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AB264130

Anti-DDX1 antibody

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Rabbit Polyclonal DDX1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DDX1 aa 500-550.

View Alternative Names

ATP-dependent RNA helicase DDX1, DEAD box protein 1, DEAD box protein retinoblastoma, DBP-RB, DDX1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB264130)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB264130)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling DDX1 with ab264130 at 1/200 dilution. Detection : DAB.

Western blot - Anti-DDX1 antibody (AB264130)
  • WB

Supplier Data

Western blot - Anti-DDX1 antibody (AB264130)

All lanes:

Western blot - Anti-DDX1 antibody (ab264130) at 0.03 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HeLa whole cell lysate at 40 µg

Lane 3:

HeLa whole cell lysate at 80 µg

Predicted band size: 82 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human DDX1 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q92499

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein DDX1 also known as DEAD-box helicase 1 is an RNA helicase with a mass of approximately 82 kDa. This protein facilitates the unwinding of RNA or RNA-DNA duplexes which is essential for various RNA metabolic processes. DDX1 possesses ATPase activity that fuels its helicase function enabling it to remodel RNA secondary structures. The protein is expressed in multiple tissues with significant expression observed in the nucleus and cytoplasm of cells.
Biological function summary

DDX1 plays a role in RNA processing and ribonucleoprotein complex formation. It participates in pre-mRNA splicing and ribosomal RNA processing contributing to efficient macromolecular synthesis. DDX1 associates with the Drosha-DGCR8 complex playing a role in microRNA biogenesis which is important for regulating gene expression. By aiding these processes DDX1 supports cellular growth and division which are fundamental to normal cellular function.

Pathways

DDX1 is involved in both RNA transport and processing pathways. In the RNA transport pathway it interacts with other proteins like Exportin-1 to mediate the translocation of RNA molecules between the nucleus and the cytoplasm. Within the RNA processing pathways DDX1 functions alongside proteins such as DDX5 to modulate splicing events that are vital for generating mature messenger RNAs. These pathways highlight DDX1's central role in managing RNA fate within the cell.

DDX1 has associations with certain cancers and neurological disorders. In particular its dysregulation is linked to neuroblastoma where altered DDX1 expression might affect cell proliferation. Additionally DDX1 interacts with BRCA1 during DNA repair processes implicating its potential involvement in breast cancer. Understanding DDX1's role in these conditions can help in exploring therapeutic strategies targeting its pathway interactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB : together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed : 24870230). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I : C) dsRNA (By similarity).. (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs.. (Microbial infection) Required for Coronavirus IBV replication.
See full target information DDX1
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