AB70252

Anti-DDX1 antibody

Name: Anti-DDX1 antibody (ab70252) | Abcam
Brand: Abcam Limited
SKU: AB70252
Price: 545 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(3 Publications)

Rabbit Polyclonal DDX1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DDX1.

View Alternative Names

ATP-dependent RNA helicase DDX1, DEAD box protein 1, DEAD box protein retinoblastoma, DBP-RB, DDX1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB70252)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB70252)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian seminoma tissue labelling DDX1 with ab70252 at 1/200 (1μg/ml). Detection : DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB70252)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal seminoma tissue labelling DDX1 with ab70252 at 1/200 (1μg/ml). Detection : DAB.

Supplier Data

Immunoprecipitation - Anti-DDX1 antibody (AB70252)

All lanes:

Immunoprecipitation - Anti-DDX1 antibody (ab70252)

Predicted band size: 82 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-DDX1 antibody (AB70252)

All lanes:

Western blot - Anti-DDX1 antibody (ab70252) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HEK293T cell lysate at 50 µg

Lane 3:

NIH3T3 cell lysate at 50 µg

Predicted band size: 82 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human DDX1. The exact immunogen used to generate this antibody is proprietary information.

Q92499

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "3-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein DDX1 also known as DEAD-box helicase 1 is an RNA helicase with a mass of approximately 82 kDa. This protein facilitates the unwinding of RNA or RNA-DNA duplexes which is essential for various RNA metabolic processes. DDX1 possesses ATPase activity that fuels its helicase function enabling it to remodel RNA secondary structures. The protein is expressed in multiple tissues with significant expression observed in the nucleus and cytoplasm of cells.

Biological function summary

DDX1 plays a role in RNA processing and ribonucleoprotein complex formation. It participates in pre-mRNA splicing and ribosomal RNA processing contributing to efficient macromolecular synthesis. DDX1 associates with the Drosha-DGCR8 complex playing a role in microRNA biogenesis which is important for regulating gene expression. By aiding these processes DDX1 supports cellular growth and division which are fundamental to normal cellular function.

Pathways

DDX1 is involved in both RNA transport and processing pathways. In the RNA transport pathway it interacts with other proteins like Exportin-1 to mediate the translocation of RNA molecules between the nucleus and the cytoplasm. Within the RNA processing pathways DDX1 functions alongside proteins such as DDX5 to modulate splicing events that are vital for generating mature messenger RNAs. These pathways highlight DDX1's central role in managing RNA fate within the cell.

DDX1 has associations with certain cancers and neurological disorders. In particular its dysregulation is linked to neuroblastoma where altered DDX1 expression might affect cell proliferation. Additionally DDX1 interacts with BRCA1 during DNA repair processes implicating its potential involvement in breast cancer. Understanding DDX1's role in these conditions can help in exploring therapeutic strategies targeting its pathway interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB : together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed : 24870230). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I : C) dsRNA (By similarity).. (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs.. (Microbial infection) Required for Coronavirus IBV replication.

See full target information DDX1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40087883

2025

Armadillo domain of ARID1A directly interacts with DNA-PKcs to couple chromatin remodeling with nonhomologous end joining (NHEJ) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Ichiro Kanno,Takayasu Kobayashi,Reiko Watanabe,Akihiro Kurimasa,Kozo Tanaka,Akira Yasui,Ayako Ui

Cell biology international 45:580-598 PubMed33200434

2021

Comparative nuclear matrix proteome analysis of skeletal muscle cells in different cellular states.

Applications

Unspecified application

Species

Unspecified reactive species

Deepika Puri,Ch V B Swamy,Jyotsna Dhawan,Rakesh K Mishra

Genome research 28:1405-1414 PubMed30108179

2018

Human proteins that interact with RNA/DNA hybrids.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel X Wang,Christopher Grunseich,Jennifer Fox,Joshua Burdick,Zhengwei Zhu,Niema Ravazian,Markus Hafner,Vivian G Cheung

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDX1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB70252)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX1 antibody (AB70252)

Immunoprecipitation - Anti-DDX1 antibody (AB70252)

Western blot - Anti-DDX1 antibody (AB70252)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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