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AB183853

Anti-DDX18 antibody

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(1 Publication)

Rabbit Polyclonal DDX18 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX18 aa 200 to C-terminus.

View Alternative Names

cPERP-D, DDX18, ATP-dependent RNA helicase DDX18, DEAD box protein 18, Myc-regulated DEAD box protein, MrDb

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-DDX18 antibody (AB183853)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX18 antibody (AB183853)

Immunofluorescent analysis of HeLa cells labeling DDX18 with ab183853 at 1/500. Image at the right stained with Hoechst 33342.

Western blot - Anti-DDX18 antibody (AB183853)
  • WB

Supplier Data

Western blot - Anti-DDX18 antibody (AB183853)

All lanes:

Western blot - Anti-DDX18 antibody (ab183853) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Lane 3:

NCI-H929 whole cell lysate at 30 µg

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DDX18 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVP1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX18 also known as DEAD-box helicase 18 functions as an RNA helicase involved in the modification of RNA structures. It unwinds RNA and is important in ribosome biogenesis. The molecular weight of DDX18 is approximately 73 kDa. DDX18 is expressed in various tissues with significant presence in the nucleolus of cells where ribosome production occurs.
Biological function summary

DDX18 plays a role in the assembly and maturation of pre-ribosomal particles. It functions within a multi-protein complex and interacts with other nucleolar proteins. This interaction is necessary for the conversion of pre-rRNA into mature rRNA which forms the structural and functional components of ribosomes essential for protein synthesis.

Pathways

DDX18 participates in the ribosome biogenesis pathway which is key to cellular protein production. It is involved in the nucleolar preribosome process alongside proteins like NOP58 and fibrillarin. DDX18 contributes to the processing of pre-rRNA intermediates critical for creating functional ribosomes which are necessary for cell growth and proliferation.

Mutations or dysregulation of DDX18 associate with certain cancers like colorectal cancer. These changes impact ribosomal RNA processing and consequently protein synthesis leading to uncontrolled cell growth. DDX18's interaction with c-Myc a protein involved in cell cycle regulation and growth highlights its connection to cancer pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable RNA-dependent helicase.
See full target information DDX18
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33846790

2021

Long non‑coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR‑320b/PBX3 axis in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Zhuang,Zhenlin Jin,Xiangkuo Zheng,Ting Jin,Lina Xiang
View all publications
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Product promise

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