AB151478

Anti-DDX19B antibody

Name: Anti-DDX19B antibody (ab151478) | Abcam
Brand: Abcam Limited
SKU: AB151478
Price: 495 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal DDX19B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDX19B aa 1-250.

View Alternative Names

DBP5, DDX19, TDBP, DDX19B, ATP-dependent RNA helicase DDX19B, DEAD box RNA helicase DEAD5, DEAD box protein 19B

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DDX19B antibody (AB151478)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDX19B antibody (AB151478)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling DDX19B with ab151478 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Unknown

Western blot - Anti-DDX19B antibody (AB151478)

10% SDS PAGE

All lanes:

Western blot - Anti-DDX19B antibody (ab151478) at 1/10000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 54 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DDX19B aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UMR2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX19B also known as DEAD (Asp-Glu-Ala-Asp) box helicase 19B is an RNA helicase enzyme that assists in RNA metabolism by unwinding RNA molecules. It is part of the DEAD-box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). This protein has a molecular weight of approximately 48 kDa. DDX19B is expressed in various human tissues most prominently in the liver and brain. This wide expression pattern suggests its involvement in essential cellular processes.

Biological function summary

DDX19B plays a vital role in RNA processing and transport. It is involved in the export of mRNA from the nucleus to the cytoplasm a critical step in gene expression. DDX19B interacts with large protein complexes such as the nuclear pore complex which facilitates the movement of molecules across the nuclear envelope. By engaging in these interactions DDX19B ensures the proper communication between the nucleus and cytoplasm maintaining cellular homeostasis.

Pathways

DDX19B is integral to the mRNA export pathway working closely with proteins such as NUP214 a nucleoporin essential for the export process. Additionally DDX19B participates in the nonsense-mediated mRNA decay (NMD) pathway which controls mRNA quality by degrading erroneous mRNA molecules. In this pathway DDX19B interacts with proteins UPF1 a critical regulator of NMD reflecting its importance in maintaining proper gene expression and preventing accumulation of faulty proteins.

DDX19B has been linked to certain cancers such as liver cancer and neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). In liver cancer DDX19B has shown aberrant expression patterns pointing to its potential role in tumorigenesis. In relation to ALS its interaction with FUS an RNA-binding protein associated with the disorder highlights its involvement in the disease's molecular mechanisms. These connections emphasize the need to further explore DDX19B as a therapeutic target in managing such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed : 10428971). Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed : 10428971).

See full target information DDX19B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111106 PubMed35858577

2022

Nuclear RNA binding regulates TDP-43 nuclear localization and passive nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren Duan,Benjamin L Zaepfel,Vasilisa Aksenova,Mary Dasso,Jeffrey D Rothstein,Petr Kalab,Lindsey R Hayes

Cell cycle (Georgetown, Tex.) 20:2195-2209 PubMed34494941

2021

Circ-PGC increases the expression of FOXR2 by targeting miR-532-3p to promote the development of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Daokui Xia,Zhen Chen,Quan Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX19B antibody

Immunocytochemistry/ Immunofluorescence - Anti-DDX19B antibody (AB151478)

Western blot - Anti-DDX19B antibody (AB151478)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise