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Rabbit Polyclonal DDX19B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX19B aa 1-250.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-DDX19B antibody (AB151478), expandable thumbnail
  • Western blot - Anti-DDX19B antibody (AB151478), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human DDX19B aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UMR2

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Chicken
Predicted
Predicted
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/5000.00000 - 1/20000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed:10428971). Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed:10428971).

Alternative names

Recommended products

Rabbit Polyclonal DDX19B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX19B aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human DDX19B aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UMR2
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DDX19B also known as DEAD (Asp-Glu-Ala-Asp) box helicase 19B is an RNA helicase enzyme that assists in RNA metabolism by unwinding RNA molecules. It is part of the DEAD-box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). This protein has a molecular weight of approximately 48 kDa. DDX19B is expressed in various human tissues most prominently in the liver and brain. This wide expression pattern suggests its involvement in essential cellular processes.

Biological function summary

DDX19B plays a vital role in RNA processing and transport. It is involved in the export of mRNA from the nucleus to the cytoplasm a critical step in gene expression. DDX19B interacts with large protein complexes such as the nuclear pore complex which facilitates the movement of molecules across the nuclear envelope. By engaging in these interactions DDX19B ensures the proper communication between the nucleus and cytoplasm maintaining cellular homeostasis.

Pathways

DDX19B is integral to the mRNA export pathway working closely with proteins such as NUP214 a nucleoporin essential for the export process. Additionally DDX19B participates in the nonsense-mediated mRNA decay (NMD) pathway which controls mRNA quality by degrading erroneous mRNA molecules. In this pathway DDX19B interacts with proteins UPF1 a critical regulator of NMD reflecting its importance in maintaining proper gene expression and preventing accumulation of faulty proteins.

Associated diseases and disorders

DDX19B has been linked to certain cancers such as liver cancer and neurodegenerative diseases like amyotrophic lateral sclerosis (ALS). In liver cancer DDX19B has shown aberrant expression patterns pointing to its potential role in tumorigenesis. In relation to ALS its interaction with FUS an RNA-binding protein associated with the disorder highlights its involvement in the disease's molecular mechanisms. These connections emphasize the need to further explore DDX19B as a therapeutic target in managing such conditions.

Product promise

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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX19B antibody (ab151478), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DDX19B antibody (ab151478)

    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling DDX19B with ab151478 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Western blot - Anti-DDX19B antibody (ab151478), expandable thumbnail

    Western blot - Anti-DDX19B antibody (ab151478)

    10% SDS PAGE

    All lanes: Western blot - Anti-DDX19B antibody (ab151478) at 1/10000 dilution

    Lane 1: A549 whole cell lysate at 30 µg

    Lane 2: HepG2 whole cell lysate at 30 µg

    Predicted band size: 54 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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