Anti-DDX21 antibody [EPR14495]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDX21 antibody [EPR14495] (AB182156)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling DDX21 with Purified ab182156 at 1 : 200 dilution (2.88 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX21 antibody [EPR14495] (AB182156)

Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue, staining DDX21 with ab182156 at 1/100 dilution. Hematoxylin was used as a counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-DDX21 antibody [EPR14495] (AB182156)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling DDX21 with Purified ab182156 at 1/60 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX21 antibody [EPR14495] (AB182156)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling DDX21 with Purified ab182156 at 1 : 200 dilution (2.89 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX21 antibody [EPR14495] (AB182156)

Immunofluorescence analysis of HepG2 cells, staining DDX21 (green) with ab182156 at 1/100 dilution. An Alexa Fluor®488 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed in 4% paraformaldehyde. Nuclei were counterstained with DAPI (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX21 antibody [EPR14495] (AB182156)

Immunofluorescence analysis of HeLa cells, staining DDX21 (red) with ab182156 at 1/100 dilution. An Alexa Fluor®555 goat anti rabbit IgG was used as a secondary antibody. Cells were fixed with -20℃ Acetone. Nuclei were counterstained with DAPI (blue).

• WB

Lab

Western blot - Anti-DDX21 antibody [EPR14495] (AB182156)

All lanes:

Western blot - Anti-DDX21 antibody [EPR14495] (ab182156) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Jurkat (Human Tcellleukemia Tlymphocyte) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

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