AB70459

Anti-DDX23 antibody

Name: Anti-DDX23 antibody (ab70459) | Abcam
Brand: Abcam Limited
SKU: AB70459
Price: 510 USD
Availability: InStock

(1 Review)

(2 Publications)

Rabbit Polyclonal DDX23 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DDX23.

View Alternative Names

Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD, DDX23

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX23 antibody (AB70459)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX23 antibody (AB70459)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX23 with ab70459 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Western blot - Anti-DDX23 antibody (AB70459)

All lanes:

Western blot - Anti-DDX23 antibody (ab70459) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DDX23. The exact immunogen used to generate this antibody is proprietary information.

Q9BUQ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Platypus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDX23 protein also known as DEAD-box helicase 23 is a member of the DEAD-box protein family. This family is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and is involved in a wide range of cellular processes. DDX23 plays a mechanical role in unwinding RNA which is essential for RNA processing. The protein has a molecular mass of approximately 82 kDa. DDX23 is expressed ubiquitously across various tissues with significant expression in the nucleus where it contributes to RNA dynamics.

Biological function summary

DEAD-box helicase 23 participates in the spliceosome complex important for pre-mRNA splicing a process that removes introns and joins exons to form mature messenger RNA. DDX23 acts during spliceosome assembly and the catalytic activation phase. Through its helicase activity it facilitates structural transitions within the spliceosome ensuring accurate splicing of pre-mRNA molecules. This function is essential for accurate gene expression and cellular function.

Pathways

DDX23 is involved in the spliceosomal pathway and related RNA processing pathways. Its role in pre-mRNA splicing links DDX23 to the regulation of gene expression impacting numerous downstream pathways. Within these pathways DDX23 interacts closely with other spliceosome-associated proteins like PRPF8 and SNRNP200. These interactions ensure the efficiency and regulation of the splicing process critical to cellular and physiological functions.

Alterations in the DDX23 function have been associated with conditions such as cancers and neurodegenerative diseases. Misregulation of DDX23 activity can lead to aberrant splicing events contributing to oncogenesis. Additionally DDX23 abnormalities correlate with neurodegenerative conditions where correct RNA processing is disrupted. In these disorders DDX23 interfaces with proteins such as PRPF8 highlighting its importance in maintaining cellular and organismal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation (PubMed : 18425142). Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA : RNA hybrid and the associated non-template single-stranded DNA (PubMed : 28076779).

See full target information DDX23

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:749144 PubMed34966670

2021

Splicing Factor DDX23, Transcriptionally Activated by E2F1, Promotes Ovarian Cancer Progression by Regulating FOXM1.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhao,Yingwei Li,Chunping Qiu,Jingying Chen,Huan Wu,Qiuman Wang,Xinyue Ma,Kun Song,Beihua Kong

Molecular therapy. Nucleic acids 24:832-844 PubMed34026327

2021

Silencing LINC01021 inhibits gastric cancer through upregulation of KISS1 expression by blocking CDK2-dependent phosphorylation of CDX2.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Rongke Jiang,Qiang Wang,Yanfang Li,Ziqian Sun,Hongying Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDX23 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX23 antibody (AB70459)

Western blot - Anti-DDX23 antibody (AB70459)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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