AB70461

Anti-DDX23 antibody

Name: Anti-DDX23 antibody (ab70461) | Abcam
Brand: Abcam Limited
SKU: AB70461
Price: 520 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal DDX23 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDX23.

View Alternative Names

Probable ATP-dependent RNA helicase DDX23, 100 kDa U5 snRNP-specific protein, DEAD box protein 23, PRP28 homolog, U5-100kD, DDX23

2 Images

Unknown

Western blot - Anti-DDX23 antibody (AB70461)

All lanes:

Western blot - Anti-DDX23 antibody (ab70461) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 96 kDa

Observed band size: 200 kDa,70 kDa,96 kDa

false

Exposure time: 30s

CiteAb

Western blot - Anti-DDX23 antibody (AB70461)

DDX23 western blot using anti-DDX23 antibody ab70461. Publication image and figure legend from Cloutier, P., Poitras, C., et al., 2017, Nat Commun, PubMed 28561026.

ab70461 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab70461 please see the product overview.

ZNHIT2 and RUVBL2 regulate the composition of the U5 snRNP.(a) Workflow of triple SILAC experiments. (b) Expression assessment of various U5 snRNP subunits following treatment with siZNHIT2 or siRUVBL2. (c–f) Interpretation of the SILAC data on scatterplots. Intensity ratio of medium-labelled proteins over unlabelled proteins (M/L) on the X axis denotes IP enrichment. Intensity ratio of medium-labelled proteins over heavy-labelled proteins (M/H) on the Y axis represents experimental variations. Marked proteins in the upper right quadrant (X>1, Y>1) are proteins enriched in the IP that were less abundant following siRNA treatment. Position of the protein used for IP is shown in all experiments.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human DDX23. The exact immunogen used to generate this antibody is proprietary information.

Q9BUQ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDX23 protein also known as DEAD-box helicase 23 is a member of the DEAD-box protein family. This family is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) and is involved in a wide range of cellular processes. DDX23 plays a mechanical role in unwinding RNA which is essential for RNA processing. The protein has a molecular mass of approximately 82 kDa. DDX23 is expressed ubiquitously across various tissues with significant expression in the nucleus where it contributes to RNA dynamics.

Biological function summary

DEAD-box helicase 23 participates in the spliceosome complex important for pre-mRNA splicing a process that removes introns and joins exons to form mature messenger RNA. DDX23 acts during spliceosome assembly and the catalytic activation phase. Through its helicase activity it facilitates structural transitions within the spliceosome ensuring accurate splicing of pre-mRNA molecules. This function is essential for accurate gene expression and cellular function.

Pathways

DDX23 is involved in the spliceosomal pathway and related RNA processing pathways. Its role in pre-mRNA splicing links DDX23 to the regulation of gene expression impacting numerous downstream pathways. Within these pathways DDX23 interacts closely with other spliceosome-associated proteins like PRPF8 and SNRNP200. These interactions ensure the efficiency and regulation of the splicing process critical to cellular and physiological functions.

Alterations in the DDX23 function have been associated with conditions such as cancers and neurodegenerative diseases. Misregulation of DDX23 activity can lead to aberrant splicing events contributing to oncogenesis. Additionally DDX23 abnormalities correlate with neurodegenerative conditions where correct RNA processing is disrupted. In these disorders DDX23 interfaces with proteins such as PRPF8 highlighting its importance in maintaining cellular and organismal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing and its phosphorylated form (by SRPK2) is required for spliceosomal B complex formation (PubMed : 18425142). Independently of its spliceosome formation function, required for the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA : RNA hybrid and the associated non-template single-stranded DNA (PubMed : 28076779).

See full target information DDX23

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:15615 PubMed28561026

2017

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Applications

Species

Unspecified reactive species

Philippe Cloutier,Christian Poitras,Mathieu Durand,Omid Hekmat,Émilie Fiola-Masson,Annie Bouchard,Denis Faubert,Benoit Chabot,Benoit Coulombe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDX23 antibody

Western blot - Anti-DDX23 antibody (AB70461)

Western blot - Anti-DDX23 antibody (AB70461)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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