AB70463

Anti-DDX24 antibody

Name: Anti-DDX24 antibody (ab70463) | Abcam
Brand: Abcam Limited
SKU: AB70463
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal DDX24 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DDX24.

View Alternative Names

ATP-dependent RNA helicase DDX24, DEAD box protein 24, DDX24

2 Images

Supplier Data

Immunoprecipitation - Anti-DDX24 antibody (AB70463)

DDX24 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70463 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab70463 at 1 μg/mL.

Lane 1 : ab70463 IP in HeLa whole cell lysate.

Lane 2 : ab70463 IP in HeLa whole cell lysate.

Lane 3 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-DDX24 antibody (ab70463)

Predicted band size: 96 kDa

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Supplier Data

Western blot - Anti-DDX24 antibody (AB70463)

All lanes:

Western blot - Anti-DDX24 antibody (ab70463) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 96 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DDX24. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to DDX24 immobilized on solid support

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX24 also known as DEAD-box helicase 24 is a member of the DEAD box protein family and functions as an ATP-dependent RNA helicase. This protein has a molecular mass of approximately 91 kDa. It is expressed in various tissues with notable levels seen in testis and heart. The expression pattern indicates its involvement in cellular processes that require tight regulation of RNA metabolism.

Biological function summary

DEAD-box helicases like DDX24 are important in RNA processing and ribosome biogenesis. DDX24 contributes to pre-rRNA processing by participating in the production and assembly of ribosomal subunits. The protein is also a part of the ribonucleoprotein complex where it influences the regulation of RNA interference processes. This suggests its essential role in RNA stability and gene expression regulation.

Pathways

DDX24 plays a significant role in the ribosome biogenesis pathway and RNA processing pathways. Both are fundamental for efficient protein synthesis. Within these pathways DDX24 interacts with other proteins like NOP56 and fibrillarin which are influential in the process of rRNA modification and folding. Such interactions are vital for the proper assembly of ribosomal subunits and their correct function in protein translation.

Dysregulation of DDX24 expression or function has been linked to certain cancers such as brain tumors where alterations in RNA processing are frequent. Moreover irregular DDX24 activity has been associated with viral infections such as HIV where the virus exploits host RNA processing machineries. This connection includes proteins like DDX6 which work with DDX24 during the viral replication cycle affecting the host's cellular environment and promoting disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent RNA helicase that plays a role in various aspects of RNA metabolism including pre-mRNA splicing and is thereby involved in different biological processes such as cell cycle regulation or innate immunity (PubMed : 24204270, PubMed : 24980433). Plays an inhibitory role in TP53 transcriptional activity and subsequently in TP53 controlled cell growth arrest and senescence by inhibiting its EP300 mediated acetylation (PubMed : 25867071). Negatively regulates cytosolic RNA-mediated innate immune signaling at least in part by affecting RIPK1/IRF7 interactions. Alternatively, possesses antiviral activity by recognizing gammaherpesvirus transcripts in the context of lytic reactivation (PubMed : 36298642). Plays an essential role in cell cycle regulation in vascular smooth muscle cells by interacting with and regulating FANCA (Fanconi anemia complementation group A) mRNA (By similarity).. (Microbial infection) Plays a positive role in HIV-1 infection by promoting Rev-dependent nuclear export of viral RNAs and their packaging into virus particles (PubMed : 24204270).

See full target information DDX24

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 19:596-608 PubMed35370459

2022

Loss-of-function Mutations K11E or E271K Lead to Novel Tumor Suppression, Implicate Nucleolar Helicase DDX24 Oncogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglin Li,Xiaoyun Chen,Jiebing Gao,Jianzhong Xian,Zhijun Li,Lei Bi,Min Yang,Shuai Yang,Hongjun Jin,Hong Shan

Molecular & cellular proteomics : MCP 20:100142 PubMed34478875

2021

Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Wu,Jennifer Nance,Feixia Chu,Thomas G Fazzio

Nature communications 8:15615 PubMed28561026

2017

R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Philippe Cloutier,Christian Poitras,Mathieu Durand,Omid Hekmat,Émilie Fiola-Masson,Annie Bouchard,Denis Faubert,Benoit Chabot,Benoit Coulombe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDX24 antibody

Immunoprecipitation - Anti-DDX24 antibody (AB70463)

Western blot - Anti-DDX24 antibody (AB70463)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise