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AB177950

Anti-DDX27 antibody [EPR13147]

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(3 Publications)

Rabbit Recombinant Monoclonal DDX27 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

cPERP-F, RHLP, HSPC259, PP3241, DDX27, Probable ATP-dependent RNA helicase DDX27, DEAD box protein 27

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] (AB177950)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] (AB177950)

Immunofluorescent analysis of HeLa cells labeling DDX27 with ab177950 at 1/50 dilution.

Western blot - Anti-DDX27 antibody [EPR13147] (AB177950)
  • WB

Supplier Data

Western blot - Anti-DDX27 antibody [EPR13147] (AB177950)

All lanes:

Western blot - Anti-DDX27 antibody [EPR13147] (ab177950) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Predicted band size: 89 kDa

false

  • Carrier free

    Anti-DDX27 antibody [EPR13147] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13147

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX27 also known as DEAD-box protein 27 operates as an important RNA helicase involved in the unwinding of RNA structures. This protein with a molecular weight of approximately 85 kDa belongs to the DEAD-box family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). It is ubiquitously expressed in various tissues prominently in the nucleolus where it functions in ribosomal RNA (rRNA) biogenesis and assembly.
Biological function summary

DEAD-box protein 27 participates in ribosome biogenesis and interacts with the pre-rRNA processing complexes. This protein acts as an ATP-dependent helicase contributing to the modification and processing of rRNA during ribosome assembly. It often associates with several nucleolar proteins forming a part of multi-protein complexes essential for the synthesis and maturation of ribosomal subunits.

Pathways

The role of DDX27 integrates mainly within the ribosome biogenesis and RNA processing pathways. It interacts with other DEAD-box proteins such as DDX18 and DDX56 which collaborate in modulating ribosomal RNA processing and ribosome assembly. These pathways are critical for maintaining proper cell proliferation and protein synthesis highlighting its functional importance in cellular processes.

Dysregulation of DDX27 is linked to certain cancers including colorectal and hepatocellular carcinoma. Aberrations in the expression of DDX27 can affect tumor progression and cellular growth through its influence on ribosome biogenesis. Additionally involvement with proteins like MYC has been observed as MYC-driven regulation of ribosomal biogenesis highlights a potential connection between DDX27 activity and oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA (PubMed : 25825154).
See full target information DDX27
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Brain communications 7:fcaf224 PubMed40574971

2025

Glycine-to-aspartic acid mutation at codon 51 in disrupts the synaptic localisation of α-synuclein and enhances its propensity for synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen West,Ammar Natalwala,Karamjit Singh Dolt,Douglas J Lamont,Melanie McMillan,Kelvin Luk,Tomoji Mashimo,Tilo Kunath

Frontiers in oncology 14:1411539 PubMed38939334

2024

miR-617 interacts with the promoter of and positively regulates its expression: implications for cancer therapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Neelanjana Sarkar,Radha Mishra,Champaka Gopal,Arun Kumar

Cancers 15: PubMed37370759

2023

METTL3-Modulated Promotes Colorectal Cancer Stemness and Metastasis through Increasing mRNA Stability by Recruiting IGF2BP1.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Chen,Buning Tian,Gui Hu,Yihang Guo
View all publications
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Product promise

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