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AB250021

Anti-DDX27 antibody [EPR13147] - BSA and Azide free

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Rabbit Recombinant Monoclonal DDX27 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples.

View Alternative Names

cPERP-F, RHLP, HSPC259, PP3241, DDX27, Probable ATP-dependent RNA helicase DDX27, DEAD box protein 27

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] - BSA and Azide free (AB250021)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX27 antibody [EPR13147] - BSA and Azide free (AB250021)

This data was developed using ab177950, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labeling DDX27 with ab177950 at 1/50 dilution.

Western blot - Anti-DDX27 antibody [EPR13147] - BSA and Azide free (AB250021)
  • WB

Supplier Data

Western blot - Anti-DDX27 antibody [EPR13147] - BSA and Azide free (AB250021)

This data was developed using ab177950, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DDX27 antibody [EPR13147] (<a href='/en-us/products/primary-antibodies/ddx27-antibody-epr13147-ab177950'>ab177950</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Predicted band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13147

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab250021 is the carrier-free version of ab177950.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX27 also known as DEAD-box protein 27 operates as an important RNA helicase involved in the unwinding of RNA structures. This protein with a molecular weight of approximately 85 kDa belongs to the DEAD-box family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). It is ubiquitously expressed in various tissues prominently in the nucleolus where it functions in ribosomal RNA (rRNA) biogenesis and assembly.
Biological function summary

DEAD-box protein 27 participates in ribosome biogenesis and interacts with the pre-rRNA processing complexes. This protein acts as an ATP-dependent helicase contributing to the modification and processing of rRNA during ribosome assembly. It often associates with several nucleolar proteins forming a part of multi-protein complexes essential for the synthesis and maturation of ribosomal subunits.

Pathways

The role of DDX27 integrates mainly within the ribosome biogenesis and RNA processing pathways. It interacts with other DEAD-box proteins such as DDX18 and DDX56 which collaborate in modulating ribosomal RNA processing and ribosome assembly. These pathways are critical for maintaining proper cell proliferation and protein synthesis highlighting its functional importance in cellular processes.

Dysregulation of DDX27 is linked to certain cancers including colorectal and hepatocellular carcinoma. Aberrations in the expression of DDX27 can affect tumor progression and cellular growth through its influence on ribosome biogenesis. Additionally involvement with proteins like MYC has been observed as MYC-driven regulation of ribosomal biogenesis highlights a potential connection between DDX27 activity and oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA (PubMed : 25825154).
See full target information DDX27
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com