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AB70821

Anti-DDX28 antibody

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(3 Publications)

Rabbit Polyclonal DDX28 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DDX28.

View Alternative Names

MDDX28, DDX28, Probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28

2 Images
Immunoprecipitation - Anti-DDX28 antibody (AB70821)
  • IP

Unknown

Immunoprecipitation - Anti-DDX28 antibody (AB70821)

Detection of Human DDX28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70821 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of DDX28 was performed using ab70821 at 1 µg/ml.

All lanes:

Immunoprecipitation - Anti-DDX28 antibody (ab70821)

Predicted band size: 60 kDa

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Western blot - Anti-DDX28 antibody (AB70821)
  • WB

Unknown

Western blot - Anti-DDX28 antibody (AB70821)

All lanes:

Western blot - Anti-DDX28 antibody (ab70821) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 60 kDa

Observed band size: 28 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DDX28. The exact immunogen used to generate this antibody is proprietary information.

Q9NUL7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDX28 protein also known as DEAD box helicase 28 is a member of the DEAD box protein family. Its molecular mass is approximately 57 kDa. This family of proteins is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DDX28 functions as an ATP-dependent RNA helicase which means it unwinds RNA structures and plays roles in rearranging ribonucleoprotein complexes. This protein is expressed in several tissues with significant expression in the liver and heart with the potential for further tissue-specific investigations.
Biological function summary

DDX28 participates in RNA processing and mitochondrial ribosome biogenesis activities. It is known to be a component of the mitoribosome a specialized ribosome for mitochondria. Being part of the mitoribosome DDX28 assists in the synthesis and assembly of proteins that are encoded by the mitochondrial genome. As helicases generally support the proper assembly and function of these complexes DDX28's role becomes important for maintaining mitochondrial translation integrity and efficiency.

Pathways

DDX28 interacts with several components within mitochondrial translation and RNA processing pathways. It associates notably with other mitochondrial ribosomal proteins forming a connection to the larger mitochondrial protein synthesis and assembly pathway. This connection is essential for the translation of mitochondrially-encoded proteins. DDX28 also has potential links with other helicase proteins such as DDX3X which leads to the interplay within various RNA helicase-involved pathways contributing to RNA metabolism and homeostasis in cells.

Mutations or dysregulation of DDX28 have been connected to mitochondrial dysfunction disorders including mitochondrial biogenesis deficiencies which impacts cellular energy homeostasis and can lead to serious conditions. Its involvement with other helicases such as DHX30 is also significant in certain neurodegenerative disorders. These associations highlight how alterations in DDX28's function can significantly affect cellular physiology and cascade into disease states when mitochondrial protein synthesis is impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function (PubMed : 25683708, PubMed : 25683715). May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity (PubMed : 11350955).
See full target information DDX28
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 16:e1008923 PubMed32735630

2020

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Paola Favia,Sara Vidoni,Vittoria Petruzzella,Massimo Zeviani

Molecular and cellular biology 40: PubMed31907278

2020

DEAD Box Protein Family Member DDX28 Is a Negative Regulator of Hypoxia-Inducible Factor 2α- and Eukaryotic Initiation Factor 4E2-Directed Hypoxic Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia L Evagelou,Olivia Bebenek,Erin J Specker,James Uniacke

Frontiers in genetics 10:1393 PubMed32082360

2020

RNase H1 Regulates Mitochondrial Transcription and Translation the Degradation of 7S RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Joanna Rusecka,Katarzyna Tońska,Massimo Zeviani
View all publications
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