AB50697

Anti-DDX39 antibody [2252C4a]

Name: Anti-DDX39 antibody [2252C4a] (ab50697) | Abcam
Brand: Abcam Limited
SKU: AB50697
Price: 505 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(1 Publication)

Mouse Monoclonal DDX39 antibody. Suitable for IP, WB and reacts with Human, Mouse, Recombinant fragment samples. Cited in 1 publication.

View Alternative Names

DDX39, DDX39A, ATP-dependent RNA helicase DDX39A, DEAD box protein 39, Nuclear RNA helicase URH49

5 Images

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Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (AB50697)

500µg HeLa cell lysate was prepared in RIPA buffer and immunoprecipitated with 5µg ab50697. The subsequent Western blot was probed with ab50697 at 1/50, followed by TrueBlot anti-mouse IgG at 1/1000. Developed by the ECL technique.

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Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (ab50697)

Predicted band size: 49 kDa

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Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)

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Western blot - Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution

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HeLa whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

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Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)

All lanes:

Western blot - Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution

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NIH3T3 whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

true

Unknown

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)

All lanes:

Western blot - Anti-DDX39 antibody [2252C4a] (ab50697)

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immunized recombinant protein

Predicted band size: 49 kDa

Observed band size: 37 kDa

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Unknown

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)

All lanes:

Western blot - Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution

All lanes:

F2480 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2252C4a

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "" }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDX39 protein also known as DDX39A or BAT1 belongs to the DEAD box family of proteins and has a molecular mass of approximately 49 kDa. Mechanically DDX39 functions as an RNA helicase which plays a critical role in the unwinding of RNA secondary structures during various RNA metabolic processes. The protein facilitates RNA splicing transport and translation by interacting with these fundamental cellular mechanisms. DDX39 is widely expressed in various tissues including liver heart and brain highlighting its importance across different biological systems.

Biological function summary

The multifunctional attributes of DDX39 contribute significantly to RNA processing events. The protein serves as an integral part of the TREX (transcription/export) complex which mediates the export of mRNA from the nucleus to the cytoplasm. By unwinding RNA DDX39 ensures proper mRNA maturation and transport essential for accurate gene expression. The involvement of DDX39 in the TREX complex highlights its central role in maintaining cellular homeostasis.

Pathways

DDX39 participates actively in gene expression and mRNA processing pathways. The protein's role in the TREX complex links it to the mRNA export pathway important for synthesizing proteins and cell cycle progression. Through its activities DDX39 interacts with related proteins such as ALYREF and UAP56 coordinating the transfer of mRNA. These interactions highlight its collaborative function within complex cellular processes.

DDX39 has connections to cancer including breast and lung cancer where altered mRNA processing contributes to tumor progression. The protein associates closely with other mRNA processing components like ALYREF where dysregulated interactions might influence cancer cell proliferation. Additionally DDX39 abnormalities have associations with spinal muscular atrophy where its involvement in RNA processing affects motor neuron health. This links DDX39 through its complex roles in both cancer and neurodegenerative disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Helicase that plays an essential role in mRNA export and is involved in multiple steps in RNA metabolism including alternative splicing (PubMed : 33941617, PubMed : 38801080). Regulates nuclear mRNA export to the cytoplasm through association with ECD (PubMed : 33941617). Also involved in spliceosomal uridine-rich small nuclear RNA (U snRNA) export by stimulating the RNA binding of adapter PHAX (PubMed : 39011894). Plays a role in the negative regulation of type I IFN production by increasing the nuclear retention of antiviral transcripts and thus reducing their protein expression (PubMed : 32393512). Independently of the interferon pathway, plays an antiviral role against alphaviruses by binding to a 5' conserved sequence element in the viral genomic RNA (PubMed : 37949067).

See full target information DDX39A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 103:1363-9 PubMed22494014

2012

DDX39 acts as a suppressor of invasion for bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minoru Kato,Min Wei,Shotaro Yamano,Anna Kakehashi,Satoshi Tamada,Tatsuya Nakatani,Hideki Wanibuchi

View all publications

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Product promise

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