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AB236015

Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DDX39 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DDX39, DDX39A, ATP-dependent RNA helicase DDX39A, DEAD box protein 39, Nuclear RNA helicase URH49

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)

Immunofluorescent staining of HepG2 cells labeling DDX39 with ab176348 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DDX39 with ab176348 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DDX39 with ab176348 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)
  • IP

Supplier Data

Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)

Western blot analysis on immunoprecipitation pellet from (1) Jurkat cells lysate or (2) 1X PBS (negative control) using ab176348 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176348).

All lanes:

Immunoprecipitation - Anti-DDX39 antibody [EPR13507(B)] (<a href='/en-us/products/primary-antibodies/ddx39-antibody-epr13507b-ab176348'>ab176348</a>)

Predicted band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13507(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab236015 is the carrier-free version of ab176348.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDX39 protein also known as DDX39A or BAT1 belongs to the DEAD box family of proteins and has a molecular mass of approximately 49 kDa. Mechanically DDX39 functions as an RNA helicase which plays a critical role in the unwinding of RNA secondary structures during various RNA metabolic processes. The protein facilitates RNA splicing transport and translation by interacting with these fundamental cellular mechanisms. DDX39 is widely expressed in various tissues including liver heart and brain highlighting its importance across different biological systems.
Biological function summary

The multifunctional attributes of DDX39 contribute significantly to RNA processing events. The protein serves as an integral part of the TREX (transcription/export) complex which mediates the export of mRNA from the nucleus to the cytoplasm. By unwinding RNA DDX39 ensures proper mRNA maturation and transport essential for accurate gene expression. The involvement of DDX39 in the TREX complex highlights its central role in maintaining cellular homeostasis.

Pathways

DDX39 participates actively in gene expression and mRNA processing pathways. The protein's role in the TREX complex links it to the mRNA export pathway important for synthesizing proteins and cell cycle progression. Through its activities DDX39 interacts with related proteins such as ALYREF and UAP56 coordinating the transfer of mRNA. These interactions highlight its collaborative function within complex cellular processes.

DDX39 has connections to cancer including breast and lung cancer where altered mRNA processing contributes to tumor progression. The protein associates closely with other mRNA processing components like ALYREF where dysregulated interactions might influence cancer cell proliferation. Additionally DDX39 abnormalities have associations with spinal muscular atrophy where its involvement in RNA processing affects motor neuron health. This links DDX39 through its complex roles in both cancer and neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus.
See full target information DDX39A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 20:20251206 PubMed40918165

2025

Peptide CCAT1-70aa promotes hepatocellular carcinoma proliferation and invasion via the MAPK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjian Wu,Ruifeng Liang,Guoman Liu,Quan Fang,Zuoming Xu,Wenchuan Li,Chuan Tan,Jian Pu
View all publications
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Product promise

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