Anti-DDX4/MVH antibody is a rabbit polyclonal antibody that is used in DDX4/MVH western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry on frozen sections (IHC-Fr). Suitable for human and mouse samples.
- Cited in over 525 publications
- Tried and trusted by researchers since 2005
- Affinity purified
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- Proceedings of the National Academy of Sciences of the United States of America 121:e24017521212024pSTAT3 activation of initiates the female pathway underlying temperature-dependent sex determination.Applications:Unspecified applicationReactive species:TrachemysPengfei Wu,Xifeng Wang,Chutian Ge,Lin Jin,Zihan Ding,Fang Liu,Ju Zhang,Fei Gao,Weiguo DuPubMed 39226347
- Scientific reports 13:208962023A comparative analysis of genes differentially expressed between rete testis cells and Sertoli cells of the mouse testis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEkaterina A Malolina,Adelya A Galiakberova,Valery V Mun,Marat S Sabirov,Erdem B Dashinimaev,Andrey Yu KulibinPubMed 38017073
- Frontiers in cell and developmental biology 11:12841842023Core binding factor subunit β plays diverse and essential roles in the male germline.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMustika Rahmawati,Kassie M Stadler,Blanca Lopez-Biladeau,Tia M Hoisington,Nathan C LawPubMed 38020932
- Medicina (Kaunas, Lithuania) 59:2023The mTOR Inhibitor Rapamycin Counteracts Follicle Activation Induced by Ovarian Cryopreservation in Murine Transplantation Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJules Bindels,Marlyne Squatrito,Laëtitia Bernet,Michelle Nisolle,Laurie Henry,Carine MunautPubMed 37629764
- Cell death & disease 14:5242023PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Chen,Qiukai E,Bo Sun,Pengxue Zhang,Nan Li,Shujia Fei,Yingnan Wang,Shuting Liu,Xiaoqiu Liu,Xuesen ZhangPubMed 37582914
- BioImpacts : BI 13:229-2402023Differentiation of human endometrial stem cells encapsulated in alginate hydrogel into oocyte-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiba Ghasemi,Somayeh Ebrahimi-Barough,Mohammad Hossein Nekoofar,Abdolreza Mohamadnia,Nasrin Lotfibakhshaiesh,Naghmeh Bahrami,Roya Karimi,Vajihe Taghdiri Nooshabadi,Mahmoud Azami,Elham Hasanzadeh,Jafar AiPubMed 37431484
- Current biology : CB 33:3017-3023.e22023Higher temperatures directly increase germ cell number, promoting feminization of red-eared slider turtles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesB Tezak,B Straková,D J Fullard,S Dupont,J McKey,C Weber,B CapelPubMed 37354900
- Stem cell research & therapy 14:1442023Metformin promotes female germline stem cell proliferation by upregulating Gata-binding protein 2 with histone β-hydroxybutyrylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Wang,Geng G Tian,Weiwei Cheng,Xiaoli Yu,Xiaoyong Li,Ji WuPubMed 37231495
- Zoological research 44:505-5212023Transcription factor triggers the SPRY1-NF-κB pathway to maintain testicular immune homeostasis and male fertility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Fei Zhang,Shi-Cheng Wan,Wen-Bo Chen,Dong-Hui Yang,Wen-Qing Liu,Ba-Lun Li,Aili Aierken,Xiao-Min Du,Yun-Xiang Li,Wen-Ping Wu,Xin-Chun Yang,Yu-Dong Wei,Na Li,Sha Peng,Xue-Ling Li,Guang-Peng Li,Jin-Lian HuaPubMed 37070575
- Communications biology 6:3892023LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Hsuan Chang,Chia-Hua Chou,Jui-Ching Wu,Keng-Mao Liao,Wei-Jia Luo,Wei-Lun Hsu,Xuan-Ren Chen,Sung-Liang Yu,Szu-Hua Pan,Pan-Chyr Yang,Kang-Yi SuPubMed 37037996
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Tested | Expected | Tested | Tested |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Platypus | Predicted | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow, Pig, Platypus | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow, Pig, Platypus | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow, Pig, Platypus | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow, Pig, Platypus | Dilution info - | Notes - |
Associated Products
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Target data
Function
ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).
Alternative names
VASA, DDX4, Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog
Recommended products
Anti-DDX4/MVH antibody is a rabbit polyclonal antibody that is used in DDX4/MVH western blot (WB), immunohistochemistry on paraffin-embedded sections (IHC-P), immunocytochemistry/immunofluorescence (ICC/IF), and immunohistochemistry on frozen sections (IHC-Fr). Suitable for human and mouse samples.
- Cited in over 525 publications
- Tried and trusted by researchers since 2005
- Affinity purified
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-DDX4 / MVH antibody (ab13840) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-Fr, IHC-P, WB.
Anti-DDX4 / MVH antibody (ab13840) has been cited over 526 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-DDX4 / MVH antibody (ab13840) has high sensitivity and specificity.
Anti-DDX4 / MVH antibody (ab13840) has 22 independent reviews from customers.
Anti-DDX4 / MVH antibody (ab13840) specifically detects DDX4 / MVH (UniProt ID: Q9NQI0; Molecular weight: 79kDa) and is sold in 100 µg selling sizes.
DDX4, also known as MVH, is a crucial germ cell marker and RNA helicase involved in spermatogenesis. Utilizing DDX4 / MVH antibodies can significantly enhance the detection and study of germ cell development. Research indicates that DDX4 plays a vital role in maintaining germline integrity and repressing transposable elements, making it essential for reproductive biology.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DDX4 also known as MVH is a protein that functions as an ATP-dependent RNA helicase. This protein with a molecular mass of approximately 79 kDa belongs to the DEAD box protein family. DDX4 localizes mostly in germ cells and is notably present in the testis of organisms like chickens. Its expression is significant in the cytoplasm of spermatocytes and oocytes where it plays an essential role in the regulation of RNA metabolism during germ cell development.
- Biological function summary
DDX4 supports RNA processes including splicing transport and stability within germ cells. The protein contributes to the assembly of protein-RNA complexes essential for cellular function. It operates in germ cell development by ensuring proper RNA handling and regulation. DDX4 also contributes to the formation of germ granules cellular structures important for RNA processing and storage in germ cells.
- Pathways
DDX4 is involved in germ cell development and differentiation. It plays a role in pathways regulating meiosis and gametogenesis cooperating with proteins like PIWIL1. Within these pathways DDX4 influences small RNA pathways which are important for gene regulation during germ cell maturation. This action underlines the protein's relevance in reproductive biology and cellular lifecycle management within the gonads.
- Associated diseases and disorders
Alterations in DDX4 expression or function have associations with infertility and germ cell tumors. Aberrant activities of DDX4 can disrupt normal germ cell development linking it to disorders affecting fertility. The protein shares connections with TDRD9 another protein associated with germ cell development implicating them in similar reproductive health issues. Understanding DDX4's function could lead to better insights into reproductive diseases and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody (ab13840)
ab13840 staining DDX4/MVH in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab13840 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
This image is courtesy of Petra Hajkova, Gurdon Institute, University of CambridgeImmunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840)
Mouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1 μg/ml) overnight at 4 degrees. ab13840 staining is shown in red and Oct4 staining is shown in green.
The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.
Western blot - Anti-DDX4 / MVH antibody (ab13840)
All lanes: Western blot - Anti-DDX4 / MVH antibody (ab13840) at 1 µg/mL
Lane 1: Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate at 20 µg
Lane 2: Human testis tissue lysate - total protein (ab30257) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 28 kDa, 47 kDa, 76 kDa
Western blot - Anti-DDX4 / MVH antibody (ab13840)
All lanes: ab13840 at 1 µg/mL
Lane 1: Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate at 20 µg
Lane 2: Human testis tissue lysate - total protein at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 47 kDa, 52 kDa, 79 kDa
Western blot - Anti-DDX4 / MVH antibody (ab13840)
All lanes: Western blot - Anti-DDX4 / MVH antibody (ab13840) at 1 µg/mL
Lanes 1 and 3: Mouse Ovary Lysate at 10 µg
Lanes 2 and 4: Mouse Embryonic Germ Cell Lysate at 20 µg
Predicted band size: 79 kDa
Observed band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)
IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)
Immunohistochemical analysis of formalin fixed paraffin embedded human testis labelling DDX4/MVH with ab13840 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
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