Anti-DDX4 / MVH antibody [EPR24148-58]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of human ovary (PMID : 26444630).The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315800 anti-CYP51A1 used at 1/2000 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-CYP51A1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Panel C : anti-CYP51A1 staining Leydig cells in human testis.

Panel D : anti-DDX4/MVH staining spermatogonia in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315800 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-LAMA1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis. Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis. Panel C : anti-LAMA1 staining basement membrane in human testis. Panel D : anti-DDX4/MVH staining spermatogonia in human testis. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab323317, ab315275 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315275 anti-LAMA1 used at 1/500 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-LAMA1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Panel C : anti-LAMA1 staining basement membrane in human testis.

Panel D : anti-DDX4/MVH staining spermatogonia in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315275 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining CYP51A1/CYP51 with ab315800 at a 1/2000 dilution, ab270534 anti-DDX4 / MVH used at 1/2000 dilution and ab315275 anti-LAMA1 used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CYP51A1/CYP51 (green; Opal^™520), anti-DDX4 / MVH (magenta; Opal^™690) and anti-LAMA1 (grey; Opal^™570) on human testis. Panel B : anti-CYP51A1/CYP51 staining leydig cells in human testis.
Panel C : anti-DDX4 / MVH staining spermatogonia and spermatocytes in human testis.
Panel D : anti-LAMA1 staining basement membrane in human testis.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315800, ab270834 and ab315275 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue.

Panel A : merged staining of anti-Vimentin (red; Opal™690), anti-CYP11A1 (cyan; Opal™520) and anti-DDX4 / MVH (green; Opal™570) on human testis.
Panel B : anti-CYP11A1 stained on Leydig cells.
Panel C : anti-DDX4 / MVH stained on all spermatogenic cell types.
Panel D : anti-Vimentin stained on Sertoli cells and fibroblasts.

The section was incubated in three rounds of staining : in the order of ab193555 (1 : 2000 dilution) and ab272494 (1 : 10000 dilution) for 30 mins, then ab270534 (1 : 2000 dlilution) for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

DAPI was used as a nuclear counter stain. Opal Polymer HRP Ms + Rb was used as a secondary.

Antigen retrieval : Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of human testis (PMID : 26444630). The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315800 anti-CYP51A1 used at 1/2000 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-CYP51A1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in mouse testis.

Panel C : anti-CYP51A1 staining Leydig cells in mouse testis.

Panel D : anti-DDX4/MVH staining spermatogonia in mouse testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315800 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of rat testis. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining CYP51A1/CYP51 with ab315800 at a 1/2000 dilution, ab270534 anti-DDX4 / MVH used at 1/2000 dilution and ab315275 anti-LAMA1 used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CYP51A1/CYP51 (green; Opal^™520), anti-DDX4 / MVH (magenta; Opal^™690) and anti-LAMA1 (grey; Opal^™570) on mouse testis. Panel B : anti-CYP51A1/CYP51 staining leydig cells in mouse testis.
Panel C : anti-DDX4 / MVH staining spermatogonia and spermatocytes in mouse testis.
Panel D : anti-LAMA1 staining basement membrane in mouse testis.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315800, ab270834 and ab315275 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of mouse ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse testis is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat testis is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of rat ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of mouse testis. The section was incubated with ab270534 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315800 anti-CYP51A1 used at 1/2000 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-CYP51A1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on rat testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in rat testis.

Panel C : anti-CYP51A1 staining Leydig cells in rat testis.

Panel D : anti-DDX4/MVH staining spermatogonia in rat testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315800 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse ovary tissue staining AMH with ab272221 at a 1 : 2000 (0.26 μg/ml) dilution, ab182744 Collagen VI used at 1 : 2000 (0.76 ug/ml) dilution and ab270534 DDX4 / MVH used at 1 : 5000 (0.1 μg/ml) dilution.

Panel A : merged staining of anti-AMH (green; Opal™570), anti-Collagen VI (magenta; Opal™690) and anti-DDX4 / MVH (gray; Opal™570) on mouse ovary.

Panel B : anti-AMH staining granulosa cells in mouse ovary.

Panel C : ant-Collagen VI staining stroma in mouse ovary.

Panel D : ant-DDX4 / MVH staining oocytes in mouse ovary.

Nuclear DNA was labelled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab272221, ab182744 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat ovary tissue staining AMH with ab272221 at a 1 : 2000 (0.26 μg/ml) dilution, ab182744 Collagen VI used at 1 : 2000 (0.76 ug/ml) dilution and ab270534 DDX4 / MVH used at 1 : 5000 (0.1 μg/ml) dilution.

Panel A : merged staining of anti-AMH (green; Opal™570), anti-Collagen VI (magenta; Opal™690) and anti-DDX4 / MVH (gray; Opal™570) on rat ovary.

Panel B : anti-AMH staining granulosa cells in rat ovary.

Panel C : ant-Collagen VI staining stroma in rat ovary.

Panel D : ant-DDX4 / MVH staining oocytes in rat ovary.

Nuclear DNA was labelled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab272221, ab182744 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining CYP51A1/CYP51 with ab315800 at a 1/2000 dilution, ab270534 anti-DDX4 / MVH used at 1/2000 dilution and ab315275 anti-LAMA1 used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CYP51A1/CYP51 (green; Opal^™520), anti-DDX4 / MVH (magenta; Opal^™690) and anti-LAMA1 (grey; Opal^™570) on rat testis. Panel B : anti-CYP51A1/CYP51 staining leydig cells in rat testis.
Panel C : anti-DDX4 / MVH staining spermatogonia and spermatocytes in rat testis.
Panel D : anti-LAMA1 staining basement membrane in rat testis.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315800, ab270834 and ab315275 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Human spleen, Human colon (PMID : 10920202).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

• WB

Lab

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : lane 1 : 10 seconds lane 2 : 7 seconds.

All lanes:

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution

Lane 1:

Rat testis tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining Hormone sensitive lipase with ab325336 at a 1/2000 (0.248 μg/ml) dilution, ab315800 anti-CYP51A1 used at a 1/2000 (0.26 μg/ml) dilution, and ab270534 anti-DDX4 used at a 1/2000 (0.238 μg/ml) dilution.

Panel A : anti-Hormone sensitive lipase (green; Opal™520), anti-CYP51A1 (magenta; Opal™690), anti-DDX4 (gray; Opal™570) on human testis.

Panel B : anti-Hormone sensitive lipase staining spermatid in human testis.

Panel C : anti-CYP51A1 staining leydig cells in human testis.

Panel D : anti-DDX4 staining spermatogonia in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325336, ab315800 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] (AB270534)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining PLCZ1 with ab325528 at a 1/500 (1.028 μg/ml) dilution, ab270534 anti-DDX4 used at a 1/2000 (0.238 μg/ml) dilution, and ab267377 anti-Wilms tumor protein used at a 1/500 (0.706 μg/ml) dilution.

Panel A : anti-PLCZ1 (green; Opal™520), anti-DDX4 (magenta; Opal™690), anti-Wilms tumor protein (gray; Opal™570) on human testis.

Panel B : anti-PLCZ1 staining spermatid in human testis.

Panel C : anti-DDX4 staining spermatogonia in human testis.

Panel D : anti-Wilms tumor protein staining Sertoli cells in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325528, ab270534 and ab267377 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.