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AB314236

Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric)

  • BOND RX™ Validated
  • 20ul selling size
  • Recombinant
  • What is this?

5

(1 Review)

|

(1 Publication)

DDX4 is an ATP-dependent RNA helicase crucial for sperm development. It helps protect genetic material in sperm cells and supports the creation of small RNA molecules needed for germline integrity.

View Alternative Names

VASA, DDX4, Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DDX4 / MVH with ab314236 at 1/5000 (0.223 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human testis.The section was incubated with ab314236 for 30 mins at room temperature, followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling DDX4 / MVH with ab314236 at 1/5000 (0.223 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human ovary.The section was incubated with ab314236 for 30 mins at room temperature, followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DDX4 / MVH with ab314236 at 1/5000 (0.223 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human colon.The section was incubated with ab314236 for 30 mins at room temperature, followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling DDX4 / MVH with ab314236 at 1/5000 (0.223 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat ovary.The section was incubated with ab314236 for 30 mins at room temperature, followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling DDX4 / MVH with ab314236 at 1/50 (22.32 ug/ml) dilution followed by ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314236 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1000 (2 ug/mL) dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling DDX4 / MVH with ab314236 at 1/50 (22.32 ug/ml) dilution followed by ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining on rat testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314236 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbedat 1000 (2 ug/mL) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling DDX4 / MVH with ab314236 at 1/5000 (0.223 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse ovary.The section was incubated with ab314236 for 30 mins at room temperature, followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DDX4 / MVH with ab314236 at 1/5000 (0.223 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat testis.The section was incubated with ab314236 for 30 mins at room temperature, followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DDX4 / MVH with ab314236 at 1/5000 (0.223 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse testis.The section was incubated with ab314236 for 30 mins at room temperature, followed by anti-Chicken IgY antibody (ab97136) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • WB

Supplier Data

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTNegative control : spleen, colon (PMID : 10920202).

All lanes:

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (ab314236) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 10 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-ab6877'>ab6877</a>) at 1/100000 dilution

Observed band size: 79 kDa

false

Exposure time: 59s

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)
  • WB

Supplier Data

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (AB314236)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen, colon (PMID : 10920202).

All lanes:

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (ab314236) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 10 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat testis tissue lysate at 10 µg

Lane 5:

Rat colon tissue lysate at 20 µg

Lane 6:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-ab6877'>ab6877</a>) at 1/100000 dilution

Observed band size: 79 kDa

false

Exposure time: 10s

  • Carrier free

    Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

EPR24148-58

Isotype

IgY

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>" } } }
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Product details

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab270534). By design, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX4 also known as MVH is a protein that functions as an ATP-dependent RNA helicase. This protein with a molecular mass of approximately 79 kDa belongs to the DEAD box protein family. DDX4 localizes mostly in germ cells and is notably present in the testis of organisms like chickens. Its expression is significant in the cytoplasm of spermatocytes and oocytes where it plays an essential role in the regulation of RNA metabolism during germ cell development.
Biological function summary

DDX4 supports RNA processes including splicing transport and stability within germ cells. The protein contributes to the assembly of protein-RNA complexes essential for cellular function. It operates in germ cell development by ensuring proper RNA handling and regulation. DDX4 also contributes to the formation of germ granules cellular structures important for RNA processing and storage in germ cells.

Pathways

DDX4 is involved in germ cell development and differentiation. It plays a role in pathways regulating meiosis and gametogenesis cooperating with proteins like PIWIL1. Within these pathways DDX4 influences small RNA pathways which are important for gene regulation during germ cell maturation. This action underlines the protein's relevance in reproductive biology and cellular lifecycle management within the gonads.

Alterations in DDX4 expression or function have associations with infertility and germ cell tumors. Aberrant activities of DDX4 can disrupt normal germ cell development linking it to disorders affecting fertility. The protein shares connections with TDRD9 another protein associated with germ cell development implicating them in similar reproductive health issues. Understanding DDX4's function could lead to better insights into reproductive diseases and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATP-dependent RNA helicase required during spermatogenesis (PubMed : 10920202, PubMed : 21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis : helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).
See full target information DDX4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

GeroScience 47:3061-3076 PubMed39672978

2024

Canagliflozin treatment prevents follicular exhaustion and attenuates hallmarks of ovarian aging in genetically heterogenous mice.

Applications

Unspecified application

Species

Unspecified reactive species

José V V Isola,Subhasri Biswas,Hashan Jayarathne,Chase R Hubbart,Jessica D Hense,Satoshi Matsuzaki,Michael T Kinter,Kenneth M Humphries,Sarah R Ocañas,Marianna Sadagurski,Michael B Stout
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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