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Chicken Recombinant Monoclonal DDX4 / MVH antibody. Carrier free. Suitable for IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] – Chicken IgY (Chimeric) – BSA and Azide free (AB307373), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] – Chicken IgY (Chimeric) – BSA and Azide free (AB307373), expandable thumbnail
  • Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (AB307373), expandable thumbnail
  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (AB307373), expandable thumbnail
  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (AB307373), expandable thumbnail

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrIHC-PWB
Human
Expected
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Tested
Tested

Tested
Tested

Species

Rat

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Mouse

Dilution info

1/100

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).

Alternative names

Recommended products

Chicken Recombinant Monoclonal DDX4 / MVH antibody. Carrier free. Suitable for IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype

IgY

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24148-58

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab307373 is a chicken monoclonal chimeric antibody.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DDX4 also known as MVH is a protein that functions as an ATP-dependent RNA helicase. This protein with a molecular mass of approximately 79 kDa belongs to the DEAD box protein family. DDX4 localizes mostly in germ cells and is notably present in the testis of organisms like chickens. Its expression is significant in the cytoplasm of spermatocytes and oocytes where it plays an essential role in the regulation of RNA metabolism during germ cell development.

Biological function summary

DDX4 supports RNA processes including splicing transport and stability within germ cells. The protein contributes to the assembly of protein-RNA complexes essential for cellular function. It operates in germ cell development by ensuring proper RNA handling and regulation. DDX4 also contributes to the formation of germ granules cellular structures important for RNA processing and storage in germ cells.

Pathways

DDX4 is involved in germ cell development and differentiation. It plays a role in pathways regulating meiosis and gametogenesis cooperating with proteins like PIWIL1. Within these pathways DDX4 influences small RNA pathways which are important for gene regulation during germ cell maturation. This action underlines the protein's relevance in reproductive biology and cellular lifecycle management within the gonads.

Associated diseases and disorders

Alterations in DDX4 expression or function have associations with infertility and germ cell tumors. Aberrant activities of DDX4 can disrupt normal germ cell development linking it to disorders affecting fertility. The protein shares connections with TDRD9 another protein associated with germ cell development implicating them in similar reproductive health issues. Understanding DDX4's function could lead to better insights into reproductive diseases and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] – Chicken IgY (Chimeric) – BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] – Chicken IgY (Chimeric) – BSA and Azide free (ab307373)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling DDX4 with ab307373 at 1/100 dilution (10.5 µg/ml) followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green).
    Confocal image showing positive staining on rat testis.
    The nuclear counterstain was DAPI (Blue). The section was incubated with ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] – Chicken IgY (Chimeric) – BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] – Chicken IgY (Chimeric) – BSA and Azide free (ab307373)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling DDX4 with ab307373 at 1/100 dilution (10.5 µg/ml) followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green).
    Confocal image showing positive staining on mouse testis.
    The nuclear counterstain was DAPI (Blue). The section was incubated with ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen, colon (PMID: 10920202).

    Exposure times:

    Lanes 1-3: 180 seconds

    Lanes 4-6: 10 seconds

    Lanes 7-9: 5 seconds

    All lanes: Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373) at 1/1000 dilution

    Lane 1: Human testis tissue lysate 10 µg

    Lane 2: Human spleen tissue lysate 20 µg

    Lane 3: Human colon tissue lysate 20 µg

    Lane 4: Mouse testis tissue lysate 10 µg

    Lane 5: Mouse colon tissue lysate 20 µg

    Lane 6: Mouse spleen tissue lysate 20 µg

    Lane 7: Rat testis tissue lysate 10 µg

    Lane 8: Rat colon tissue lysate 20 µg

    Lane 9: Rat spleen tissue lysate 20 µg

    Observed band size: 79 kDa

    Exposure time: 180s

  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling DDX4 with ab307373 at 1/5000 dilution (0.21 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining in rat testis is observed.
    The section was incubated with ab307373 for 30 mins at room temperature, followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is reasy to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue labeling DDX4 with ab307373 at 1/5000 dilution (0.21 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining in oocytes of rat ovary is observed.
    The section was incubated with ab307373 for 30 mins at room temperature, followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is reasy to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling DDX4 with ab307373 at 1/5000 dilution (0.21 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining in mouse testis is observed.
    The section was incubated with ab307373 for 30 mins at room temperature, followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is reasy to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue labeling DDX4 with ab307373 at 1/5000 dilution (0.21 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining in oocytes of mouse ovary is observed.
    The section was incubated with ab307373 for 30 mins at room temperature, followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is reasy to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DDX4 with ab307373 at 1/5000 dilution (0.21 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining in human testis is observed.
    The section was incubated with ab307373 for 30 mins at room temperature, followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is reasy to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling DDX4 with ab307373 at 1/5000 dilution (0.21 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Cytoplasmic staining in oocytes of human ovary is observed.
    The section was incubated with ab307373 for 30 mins at room temperature, followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is reasy to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373), expandable thumbnail

    Immunohistochemistry - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY - Chimeric - BSA and Azide free (ab307373)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling DDX4 with ab307373 at 1/5000 dilution (0.21 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Negative control: no staining in human colon. 
    The section was incubated with ab307373 for 30 mins at room temperature, followed by anti-chicken IgG antibody (Rabbit Anti-Chicken IgY H&L ab97136) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is reasy to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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