AB250572

Anti-DDX41 antibody [EPR14298] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal DDX41 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ABS, DDX41, Probable ATP-dependent RNA helicase DDX41, DEAD box protein 41, DEAD box protein abstrakt homolog

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

This data was developed using ab182007, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells (fixative -20°C Acetone) labeling DDX41 with ab182007 at a 1/250 dilution. Goat anti rabbit IgG (Dylight 488) secondary used at a 1/200 diution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

This data was developed using ab182007, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling DDX41 with ab182007 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

This data was developed using ab182007, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DDX41 antibody [EPR14298] (<a href='/en-us/products/primary-antibodies/ddx41-antibody-epr14298-ab182007'>ab182007</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 70 kDa

false

Supplier Data

Western blot - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

This data was developed using ab182007, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DDX41 antibody [EPR14298] (<a href='/en-us/products/primary-antibodies/ddx41-antibody-epr14298-ab182007'>ab182007</a>) at 1/10000 dilution

All lanes:

Jar cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 70 kDa

false

Unconjugated

Anti-DDX41 antibody [EPR14298]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14298

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "For antigen retrieval, we recommend Heat Induced Epitope Retrieval (HIER) by using Decloaking Chamber/Pressure Cooker. Run the heat program for 30 seconds at 125 degree C, then let the slides cool down to room temperature.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250572 is the carrier-free version of ab182007.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDX41 protein also known as DEAD Box Protein 41 is a member of the DEAD-box RNA helicase family. This protein weighs approximately 82 kDa. It is involved in various RNA processing activities including alteration of RNA secondary structure during spliceosome assembly ribosome biogenesis and RNA transport. DDX41 is expressed in a wide range of tissues with particularly high levels in bone marrow and immune cells. By functioning as an RNA helicase it plays a central role in the regulation of fundamental biological processes like RNA stability and translation.

Biological function summary

DDX41 plays a significant role in the innate immune response. It acts as a cytosolic DNA sensor that is involved in the early detection of viral and bacterial DNA. DDX41 interacts with the stimulator of interferon genes (STING) adaptor protein forming an important part of the DNA sensing complex that activates type I interferon responses. By recognizing and binding to foreign DNA within the cytoplasm it helps initiate an antiviral state promoting the activation of immune responses to foreign pathogens.

Pathways

The involvement of DDX41 extends to the innate immune signaling pathway and the type I interferon production pathway. Within these pathways DDX41 interacts directly with other important proteins like STING and TBK1 contributing to the signal transduction that leads to interferon production and subsequent immune activation. These pathways are important for mounting an effective defense against infections connecting DDX41 with the broader regulatory network of proteins involved in pathogen recognition and immune response modulation.

Mutations in the DDX41 gene correlate with certain hematologic conditions including myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). Disruptions in the normal function of DDX41 can affect its role in immune signaling potentially leading to aberrant cell growth and impaired immune functions. Additionally interactions with proteins such as STING during immune responses imply that dysregulation in DDX41 activity could elucidate further insights into other immune-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional protein that participates in many aspects of cellular RNA metabolism. Plays pivotal roles in innate immune sensing and hematopoietic homeostasis (PubMed : 34473945). Recognizes foreign or self-nucleic acids generated during microbial infection, thereby initiating anti-pathogen responses (PubMed : 23222971). Mechanistically, phosphorylation by BTK allows binding to dsDNA leading to interaction with STING1 (PubMed : 25704810). Modulates the homeostasis of dsDNA through its ATP-dependent DNA-unwinding activity and ATP-independent strand-annealing activity (PubMed : 35613581). In turn, induces STING1-mediated type I interferon and cytokine responses to DNA and DNA viruses (PubMed : 35613581). Selectively modulates the transcription of certain immunity-associated genes by regulating their alternative splicing (PubMed : 33650667). Binds to RNA (R)-loops, structures consisting of DNA/RNA hybrids and a displaced strand of DNA that occur during transcription, and prevents their accumulation, thereby maintaining genome stability (PubMed : 36229594). Also participates in pre-mRNA splicing, translational regulation and snoRNA processing, which is essential for ribosome biogenesis (PubMed : 36229594, PubMed : 36780110).

See full target information DDX41

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX41 antibody [EPR14298] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

Western blot - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

Western blot - Anti-DDX41 antibody [EPR14298] - BSA and Azide free (AB250572)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise