AB225870

Anti-DDX47 antibody

Name: Anti-DDX47 antibody (ab225870) | Abcam
Brand: Abcam Limited
SKU: AB225870

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal DDX47 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDX47 aa 400 to C-terminus.

View Alternative Names

Probable ATP-dependent RNA helicase DDX47, DEAD box protein 47, DDX47

2 Images

Supplier Data

Immunoprecipitation - Anti-DDX47 antibody (AB225870)

DDX47 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225870 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225870 at 0.4 μg/ml.

Lane 1 : ab225870 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DDX47 antibody (ab225870)

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-DDX47 antibody (AB225870)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-DDX47 antibody (ab225870) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DDX47 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H0S4

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX47 also known as DEAD box protein 47 functions as a putative RNA helicase involved in the unwinding of RNA secondary structures. This protein weighing approximately 52 kDa contains the conserved D-E-A-D amino acid motif characteristic of DEAD box proteins. It expresses broadly across various tissues but shows significant levels in the nucleolus where it plays roles in nucleolar activities.

Biological function summary

This protein participates in ribosome biogenesis by assisting in the maturation of ribosomal RNA. DDX47 as part of ribonucleoprotein complexes facilitates the cleavage of precursor rRNA which is necessary for the production of functional ribosomal subunits. This process is essential for maintaining high rates of protein synthesis in rapidly growing cells thereby influencing cellular growth and proliferation.

Pathways

RNA metabolism and ribosome biogenesis include DDX47 as an important component. In these pathways it interacts with proteins such as NOP56 and NOLC1 which are integral to ribosomal RNA processing. Through its role in these pathways DDX47 influences transcriptional and translational regulation which are fundamental processes in cell physiology.

Alterations in DDX47 expression link to cancer and ribosomopathies. Abnormal activity or dysregulation of DDX47 can affect cell proliferation contributing to tumorigenesis. This protein connects to oncogenic proteins such as MYC which is associated with cell cycle regulation and cancer progression. Understanding these interactions provides insights into how DDX47 may contribute to disease pathogenesis and presents potential diagnostic or therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Required for efficient ribosome biogenesis (By similarity). May have a role in mRNA splicing (PubMed : 16963496). Involved in apoptosis (PubMed : 15977068).

See full target information DDX47

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:100953 PubMed36809766

2023

Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhao,Li Cao,Wen-Jun Gu,Lei Li,Zhong-Zhong Chen,Jie Xiang,Ze-Yu Zhou,Bo Xu,Wei-Dong Zang,Xiang-Yu Zhou,Jing Cao,Kun Sun,Jian-Yuan Zhao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX47 antibody

Immunoprecipitation - Anti-DDX47 antibody (AB225870)

Western blot - Anti-DDX47 antibody (AB225870)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise