AB53216

Anti-DDX5 antibody [2257C3a]

KO Validated
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(4 Publications)

Mouse Monoclonal DDX5 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDX5.

View Alternative Names

G17P1, HELR, HLR1, DDX5, Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68

4 Images

Unknown

Western blot - Anti-DDX5 antibody [2257C3a] (AB53216)

All lanes:

Western blot - Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

anti-Mouse IgG at 1/2500 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

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Western blot - Anti-DDX5 antibody [2257C3a] (AB53216)

All lanes:

Western blot - Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution

All lanes:

NIH3T3 whole cell lysate at 25 µg

Secondary

All lanes:

anti-mouse IgG at 1/2500 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

true

Unknown

Western blot - Anti-DDX5 antibody [2257C3a] (AB53216)

All lanes:

Western blot - Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution

All lanes:

F2408 whole cell lysate at 25 µg

Secondary

All lanes:

anti-Mouse IgG at 1/2500 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

true

Lab

Western blot - Anti-DDX5 antibody [2257C3a] (AB53216)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : DDX5 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : MCF7 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab53216 observed at 69 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab53216 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. ab53216 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-DDX5 antibody [2257C3a] (ab53216)

Predicted band size: 69 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2257C3a

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human DDX5. The exact immunogen used to generate this antibody is proprietary information.

P17844

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab53216 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX5 also known as DEAD-box helicase 5 or p68 is an RNA helicase important to various RNA processing tasks. With a molecular mass of approximately 68 kDa DDX5 unwinds RNA secondary structures using energy from ATP hydrolysis. It expresses widely in human tissues with higher levels observed in regions of active cellular proliferation. The expression pattern reflects its significant involvement in pathways that require dynamic RNA structure modifications.

Biological function summary

The function of DDX5 facilitates RNA splicing transcription and ribosome biogenesis. DDX5 is a component of various RNA processing complexes interacting with other proteins to modify pre-mRNA and other RNA molecules. It plays a role in modulating gene expression and ensuring the proper processing of precursor RNA transcripts. This makes DDX5 important in cellular differentiation and development influencing stem cell renewal and pluripotency.

Pathways

DDX5 participates in the Wnt/β-catenin and p53 signaling pathways. In the Wnt/β-catenin pathway DDX5 interacts with proteins such as β-catenin to influence gene transcription involved in cell growth and differentiation. Within the p53 pathway DDX5 modulates the activity of p53 a protein known for its role in DNA damage response and apoptosis. These interactions position DDX5 at the intersection of multiple regulatory networks that govern cellular proliferation and survival.

Aberrant DDX5 expression links to cancer and neurodegenerative diseases. Overexpression or dysregulation of DDX5 has been observed in various cancers including breast cancer where it can interact with proteins such as estrogen receptor alpha promoting tumor progression. In neurodegenerative disorders particularly amyotrophic lateral sclerosis (ALS) DDX5 forms toxic aggregates with TDP-43 a protein important in RNA binding and processing. Understanding DDX5's involvement in these diseases offers insight into potential therapeutic targets and diagnostic markers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

See full target information DDX5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:770-795 PubMed38182816

2024

DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xu,Li-Yuan Liu,Fei-Jie Zhi,Yin-Juan Song,Zi-Hui Zhang,Bin Li,Fu-Ying Zheng,Peng-Cheng Gao,Su-Zi Zhang,Yu-Yu Zhang,Ying Zhang,Ying Qiu,Bo Jiang,Yong-Qing Li,Chen Peng,Yue-Feng Chu

PLoS pathogens 17:e1009530 PubMed33909701

2021

The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NFκB transcripts to dampen antiviral innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xu,Yunhong Cai,ZhenBang Ma,Bo Jiang,Wenxiao Liu,Jing Cheng,Nannan Guo,Zishu Wang,Joshua E Sealy,Cuiping Song,Xiaojia Wang,Yongqing Li

Nucleic acids research 42:7186-200 PubMed24792155

2014

DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1.

Applications

Unspecified application

Species

Unspecified reactive species

Olof J Pettersson,Lars Aagaard,Diana Andrejeva,Rune Thomsen,Thomas G Jensen,Christian K Damgaard

Genes & development 24:2543-55 PubMed20966046

2010

Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjie Yao,Kevin Brick,Yvonne Evrard,Tiaojiang Xiao,R Daniel Camerini-Otero,Gary Felsenfeld

View all publications

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