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AB10261

Anti-DDX5 antibody

3

(3 Reviews)

|

(27 Publications)

Goat Polyclonal DDX5 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human DDX5 aa 600 to C-terminus.

View Alternative Names

G17P1, HELR, HLR1, DDX5, Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68

1 Images
Western blot - Anti-DDX5 antibody (AB10261)
  • WB

Supplier Data

Western blot - Anti-DDX5 antibody (AB10261)

All lanes:

Western blot - Anti-DDX5 antibody (ab10261) at 0.5 µg/mL

Lane 1:

A431 cell nuclear lysate at 35 µg

Lane 2:

HeLa cell nuclear lysate at 35 µg

Lane 3:

HepG2 cell nuclear lysate at 35 µg

Lane 4:

Jurkat cell nuclear lysate at 35 µg

Lane 5:

NIH3T3 cell nuclear lysate at 35 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human DDX5 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17844

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX5 also known as DEAD-box helicase 5 or p68 is an RNA helicase important to various RNA processing tasks. With a molecular mass of approximately 68 kDa DDX5 unwinds RNA secondary structures using energy from ATP hydrolysis. It expresses widely in human tissues with higher levels observed in regions of active cellular proliferation. The expression pattern reflects its significant involvement in pathways that require dynamic RNA structure modifications.
Biological function summary

The function of DDX5 facilitates RNA splicing transcription and ribosome biogenesis. DDX5 is a component of various RNA processing complexes interacting with other proteins to modify pre-mRNA and other RNA molecules. It plays a role in modulating gene expression and ensuring the proper processing of precursor RNA transcripts. This makes DDX5 important in cellular differentiation and development influencing stem cell renewal and pluripotency.

Pathways

DDX5 participates in the Wnt/β-catenin and p53 signaling pathways. In the Wnt/β-catenin pathway DDX5 interacts with proteins such as β-catenin to influence gene transcription involved in cell growth and differentiation. Within the p53 pathway DDX5 modulates the activity of p53 a protein known for its role in DNA damage response and apoptosis. These interactions position DDX5 at the intersection of multiple regulatory networks that govern cellular proliferation and survival.

Aberrant DDX5 expression links to cancer and neurodegenerative diseases. Overexpression or dysregulation of DDX5 has been observed in various cancers including breast cancer where it can interact with proteins such as estrogen receptor alpha promoting tumor progression. In neurodegenerative disorders particularly amyotrophic lateral sclerosis (ALS) DDX5 forms toxic aggregates with TDP-43 a protein important in RNA binding and processing. Understanding DDX5's involvement in these diseases offers insight into potential therapeutic targets and diagnostic markers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
See full target information DDX5
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3292 PubMed40195294

2025

RNA G-quadruplexes control mitochondria-localized mRNA translation and energy metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Leïla Dumas,Sauyeun Shin,Quentin Rigaud,Marie Cargnello,Beatriz Hernández-Suárez,Pauline Herviou,Nathalie Saint-Laurent,Marjorie Leduc,Morgane Le Gall,David Monchaud,Erik Dassi,Anne Cammas,Stefania Millevoi

EMBO reports 25:770-795 PubMed38182816

2024

DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xu,Li-Yuan Liu,Fei-Jie Zhi,Yin-Juan Song,Zi-Hui Zhang,Bin Li,Fu-Ying Zheng,Peng-Cheng Gao,Su-Zi Zhang,Yu-Yu Zhang,Ying Zhang,Ying Qiu,Bo Jiang,Yong-Qing Li,Chen Peng,Yue-Feng Chu

Database : the journal of biological databases and curation 2023: PubMed38128543

2023

SplicingLore: a web resource for studying the regulation of cassette exons by human splicing factors.

Applications

Unspecified application

Species

Unspecified reactive species

Hélène Polvèche,Jessica Valat,Nicolas Fontrodona,Audrey Lapendry,Valentine Clerc,Stéphane Janczarski,Franck Mortreux,Didier Auboeuf,Cyril F Bourgeois

Cell reports 40:111267 PubMed36044855

2022

The RNA helicase DDX5 cooperates with EHMT2 to sustain alveolar rhabdomyosarcoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Alberto Gualtieri,Valeria Bianconi,Alessandra Renzini,Luisa Pieroni,Valerio Licursi,Chiara Mozzetta

Nucleic acids research 50:9226-9246 PubMed36039747

2022

RNA helicase-dependent gene looping impacts messenger RNA processing.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Terrone,Jessica Valat,Nicolas Fontrodona,Guillaume Giraud,Jean-Baptiste Claude,Emmanuel Combe,Audrey Lapendry,Hélène Polvèche,Lamya Ben Ameur,Arnaud Duvermy,Laurent Modolo,Pascal Bernard,Franck Mortreux,Didier Auboeuf,Cyril F Bourgeois

iScience 24:103055 PubMed34541469

2021

STING nuclear partners contribute to innate immune signaling responses.

Applications

Unspecified application

Species

Unspecified reactive species

Charles R Dixon,Poonam Malik,Jose I de Las Heras,Natalia Saiz-Ros,Flavia de Lima Alves,Mark Tingey,Eleanor Gaunt,A Christine Richardson,David A Kelly,Martin W Goldberg,Greg J Towers,Weidong Yang,Juri Rappsilber,Paul Digard,Eric C Schirmer

The EMBO journal 40:e106018 PubMed33634895

2021

BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair‡.

Applications

Unspecified application

Species

Unspecified reactive species

Gaetana Sessa,Belén Gómez-González,Sonia Silva,Carmen Pérez-Calero,Romane Beaurepere,Sonia Barroso,Sylvain Martineau,Charlotte Martin,Åsa Ehlén,Juan S Martínez,Bérangère Lombard,Damarys Loew,Stephan Vagner,Andrés Aguilera,Aura Carreira

Scientific reports 10:19634 PubMed33184340

2020

Human pericentromeric tandemly repeated DNA is transcribed at the end of oocyte maturation and is associated with membraneless mitochondria-associated structures.

Applications

Unspecified application

Species

Unspecified reactive species

M A Dobrynin,N M Korchagina,A D Prjibelski,D Shafranskaya,D I Ostromyshenskii,K Shunkina,I Stepanova,A V Kotova,O I Podgornaya,N I Enukashvily

International journal of molecular sciences 21: PubMed33096920

2020

First Identification of RNA-Binding Proteins That Regulate Alternative Exons in the Dystrophin Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Miro,Anne-Laure Bougé,Eva Murauer,Emmanuelle Beyne,Dylan Da Cunha,Mireille Claustres,Michel Koenig,Sylvie Tuffery-Giraud

Nature communications 11:3045 PubMed32546717

2020

Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1.

Applications

Unspecified application

Species

Unspecified reactive species

Lamya Ben Ameur,Paul Marie,Morgan Thenoz,Guillaume Giraud,Emmanuel Combe,Jean-Baptiste Claude,Sebastien Lemaire,Nicolas Fontrodona,Hélène Polveche,Marine Bastien,Antoine Gessain,Eric Wattel,Cyril F Bourgeois,Didier Auboeuf,Franck Mortreux
View all publications
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