AB92917

Anti-DDX5 antibody

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(1 Publication)

Mouse Polyclonal DDX5 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DDX5.

View Alternative Names

G17P1, HELR, HLR1, DDX5, Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody (AB92917)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody (AB92917)

Immunofluorescence analysis of HeLa cells labelled with ab92917 at 10 μg/ml.

Western blot - Anti-DDX5 antibody (AB92917)

WB

Unknown

Western blot - Anti-DDX5 antibody (AB92917)

All lanes:

Western blot - Anti-DDX5 antibody (ab92917) at 1/500 dilution

All lanes:

Mouse raw 264.7 cell lysate at 50 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB

Immunogen

Recombinant Full Length Protein corresponding to Human DDX5.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX5 also known as DEAD-box helicase 5 or p68 is an RNA helicase important to various RNA processing tasks. With a molecular mass of approximately 68 kDa DDX5 unwinds RNA secondary structures using energy from ATP hydrolysis. It expresses widely in human tissues with higher levels observed in regions of active cellular proliferation. The expression pattern reflects its significant involvement in pathways that require dynamic RNA structure modifications.

Biological function summary

The function of DDX5 facilitates RNA splicing transcription and ribosome biogenesis. DDX5 is a component of various RNA processing complexes interacting with other proteins to modify pre-mRNA and other RNA molecules. It plays a role in modulating gene expression and ensuring the proper processing of precursor RNA transcripts. This makes DDX5 important in cellular differentiation and development influencing stem cell renewal and pluripotency.

Pathways

DDX5 participates in the Wnt/β-catenin and p53 signaling pathways. In the Wnt/β-catenin pathway DDX5 interacts with proteins such as β-catenin to influence gene transcription involved in cell growth and differentiation. Within the p53 pathway DDX5 modulates the activity of p53 a protein known for its role in DNA damage response and apoptosis. These interactions position DDX5 at the intersection of multiple regulatory networks that govern cellular proliferation and survival.

Aberrant DDX5 expression links to cancer and neurodegenerative diseases. Overexpression or dysregulation of DDX5 has been observed in various cancers including breast cancer where it can interact with proteins such as estrogen receptor alpha promoting tumor progression. In neurodegenerative disorders particularly amyotrophic lateral sclerosis (ALS) DDX5 forms toxic aggregates with TDP-43 a protein important in RNA binding and processing. Understanding DDX5's involvement in these diseases offers insight into potential therapeutic targets and diagnostic markers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

See full target information DDX5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of fungi (Basel, Switzerland) 5: PubMed30939757

2019

and non- Isolates from Bloodstream Have Different Capacities to Induce Neutrophil Extracellular Traps.

Applications

Unspecified application

Species

Unspecified reactive species

Lizbeth Campos-Garcia,Rocio Jimena Jimenez-Valdes,Romel Hernandez-Bello,Jose Palma-Nicolas,Gloria Maria Gonzalez,Alejandro Sanchez-Gonzalez

View all publications

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Product promise

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