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AB62255

Anti-DDX5 (phospho Y593) antibody

4

(1 Review)

|

(3 Publications)

Rabbit Polyclonal DDX5 phospho Y593 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DDX5 phospho Y593 aa 550 to C-terminus.

View Alternative Names

G17P1, HELR, HLR1, DDX5, Probable ATP-dependent RNA helicase DDX5, DEAD box protein 5, RNA helicase p68

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-DDX5 (phospho Y593) antibody (AB62255)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDX5 (phospho Y593) antibody (AB62255)

ab62255 at 1/500 - 1/1000 dilution staining DDX5 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human DDX5 phospho Y593 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17844

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab62255 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX5 also known as DEAD-box helicase 5 or p68 is an RNA helicase important to various RNA processing tasks. With a molecular mass of approximately 68 kDa DDX5 unwinds RNA secondary structures using energy from ATP hydrolysis. It expresses widely in human tissues with higher levels observed in regions of active cellular proliferation. The expression pattern reflects its significant involvement in pathways that require dynamic RNA structure modifications.
Biological function summary

The function of DDX5 facilitates RNA splicing transcription and ribosome biogenesis. DDX5 is a component of various RNA processing complexes interacting with other proteins to modify pre-mRNA and other RNA molecules. It plays a role in modulating gene expression and ensuring the proper processing of precursor RNA transcripts. This makes DDX5 important in cellular differentiation and development influencing stem cell renewal and pluripotency.

Pathways

DDX5 participates in the Wnt/β-catenin and p53 signaling pathways. In the Wnt/β-catenin pathway DDX5 interacts with proteins such as β-catenin to influence gene transcription involved in cell growth and differentiation. Within the p53 pathway DDX5 modulates the activity of p53 a protein known for its role in DNA damage response and apoptosis. These interactions position DDX5 at the intersection of multiple regulatory networks that govern cellular proliferation and survival.

Aberrant DDX5 expression links to cancer and neurodegenerative diseases. Overexpression or dysregulation of DDX5 has been observed in various cancers including breast cancer where it can interact with proteins such as estrogen receptor alpha promoting tumor progression. In neurodegenerative disorders particularly amyotrophic lateral sclerosis (ALS) DDX5 forms toxic aggregates with TDP-43 a protein important in RNA binding and processing. Understanding DDX5's involvement in these diseases offers insight into potential therapeutic targets and diagnostic markers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
See full target information DDX5 phospho Y593
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112219 PubMed40224004

2025

Supinoxin blocks small cell lung cancer progression by inhibiting mitochondrial respiration through DDX5.

Applications

Unspecified application

Species

Unspecified reactive species

Subhadeep Das,Maria P Zea,Matthew P Russon,Zheng Xing,Sandra Torregrosa-Allen,Heidi E Cervantes,Haley Anne Harper,Bennett D Elzey,Elizabeth J Tran

Molecular therapy : the journal of the American So 28:946-962 PubMed31982037

2020

Long Noncoding RNA NHEG1 Drives β-Catenin Transactivation and Neuroblastoma Progression through Interacting with DDX5.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhao,Dan Li,Feng Yang,Heng Lian,Jianqun Wang,Xiaojing Wang,Erhu Fang,Huajie Song,Anpei Hu,Yanhua Guo,Yang Liu,Hongjun Li,Yajun Chen,Kai Huang,Liduan Zheng,Qiangsong Tong

Molecular and cellular biology 32:3140-52 PubMed22665494

2012

mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling through its protein partner Ddx5/p68 in mouse spermatogonial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gayatri Arun,Vijay Suresh Akhade,Sainitin Donakonda,Manchanahalli R Satyanarayana Rao
View all publications
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