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AB103545

Anti-DDX53/CT26 antibody

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(1 Publication)

Rabbit Polyclonal DDX53/CT26 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DDX53.

View Alternative Names

CAGE, DDX53, Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, DEAD box protein 53, DEAD box protein CAGE, CT26

2 Images
Western blot - Anti-DDX53/CT26 antibody (AB103545)
  • WB

Unknown

Western blot - Anti-DDX53/CT26 antibody (AB103545)

All lanes:

Western blot - Anti-DDX53/CT26 antibody (ab103545) at 1 µg/mL

Lane 1:

DDX53/CT26 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 71 kDa

false

Western blot - Anti-DDX53/CT26 antibody (AB103545)
  • WB

Unknown

Western blot - Anti-DDX53/CT26 antibody (AB103545)

All lanes:

Western blot - Anti-DDX53/CT26 antibody (ab103545) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 119 kDa,41 kDa,67 kDa,68 kDa,71 kDa,73 kDa

Observed band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DDX53.

Q86TM3

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX53 also known as CT26 is a member of the DEAD box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). This protein functions primarily as an ATP-dependent RNA helicase facilitating the unwinding of RNA structures. The molecular weight of DDX53 is approximately 58 kDa. Its expression is found mainly in various tumor tissues making it a cancer/testis antigen. It is not commonly expressed in normal tissues except for the testis highlighting its potential as a biomarker for cancer diagnostics.
Biological function summary

DDX53/CT26 plays a role in cellular processes related to cell growth and proliferation. It participates in mRNA metabolism and splicing though it is not necessarily part of a known stable complex. The role of DDX53 in mRNA processing links it to the regulation of gene expression at a post-transcriptional level influencing cell cycle control and proliferation dynamics. Such regulatory functions are critical in understanding how aberrant expression might contribute to tumorigenesis.

Pathways

DDX53 is involved in pathways that regulate gene expression and proliferation such as the mRNA splicing pathway. Its interaction with other RNA-binding proteins might modulate splicing events important for protein expression patterns that sustain cell growth. DDX53 relates to proteins involved in the regulation of RNA metabolism such as other DEAD box family proteins which ensure accurate splicing and mRNA turnover. By affecting these pathways DDX53 can impact cellular growth control mechanisms.

DDX53's aberrant expression is linked to certain cancers most prominently in lung cancer and melanoma. Its expression correlates with tumor presence and progression. The interaction of DDX53 with testis-specific proteins in tumors makes it a relevant target for cancer immunotherapy. Furthermore its specificity to cancerous tissues over normal ones barring the testis positions DDX53 as a potentially valuable target for cancer vaccine development leveraging immune responses against tumor cells expressing this antigen.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC medical genomics 16:5 PubMed36635662

2023

Disruption of DDX53 coding sequence has limited impact on iPSC-derived human NGN2 neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Faheem,Eric Deneault,Roumiana Alexandrova,Deivid C Rodrigues,Giovanna Pellecchia,Carole Shum,Mehdi Zarrei,Alina Piekna,Wei Wei,Jennifer L Howe,Bhooma Thiruvahindrapuram,Sylvia Lamoureux,P Joel Ross,Clarrisa A Bradley,James Ellis,Stephen W Scherer
View all publications

Product promise

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