AB313333

Anti-DDX53/CT26 antibody [EPR27384-107]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal DDX53/CT26 antibody. Suitable for WB and reacts with Human, Transfected cell lysate - Human samples.

View Alternative Names

CAGE, DDX53, Probable ATP-dependent RNA helicase DDX53, Cancer-associated gene protein, Cancer/testis antigen 26, DEAD box protein 53, DEAD box protein CAGE, CT26

4 Images

Supplier Data

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : kidney (PMID : 11922625).

In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (ab313333) at 1/1000 dilution

Lane 1:

Human testis tissue nuclear fraction at 40 µg

Lane 2:

Human kidney tissue nuclear fraction at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 74 kDa

true

Exposure time: 70s

Supplier Data

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (ab313333) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

K562 transfected with siRNA specifically targeti DDX53/CT26 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : PANC-1 (PMID : 12531476), MCF7 (PMID : 28152297), SK-BR-3 (PMID : 28152297). In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (ab313333) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 50 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 4:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 74 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human DDX43.

In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (ab313333) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human DDX53 expression vector containi a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human DDX43 expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 55 kDa,74 kDa

false

Exposure time: 3s

Carrier free

Anti-DDX53/CT26 antibody [EPR27384-107] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27384-107

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX53 also known as CT26 is a member of the DEAD box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). This protein functions primarily as an ATP-dependent RNA helicase facilitating the unwinding of RNA structures. The molecular weight of DDX53 is approximately 58 kDa. Its expression is found mainly in various tumor tissues making it a cancer/testis antigen. It is not commonly expressed in normal tissues except for the testis highlighting its potential as a biomarker for cancer diagnostics.

Biological function summary

DDX53/CT26 plays a role in cellular processes related to cell growth and proliferation. It participates in mRNA metabolism and splicing though it is not necessarily part of a known stable complex. The role of DDX53 in mRNA processing links it to the regulation of gene expression at a post-transcriptional level influencing cell cycle control and proliferation dynamics. Such regulatory functions are critical in understanding how aberrant expression might contribute to tumorigenesis.

Pathways

DDX53 is involved in pathways that regulate gene expression and proliferation such as the mRNA splicing pathway. Its interaction with other RNA-binding proteins might modulate splicing events important for protein expression patterns that sustain cell growth. DDX53 relates to proteins involved in the regulation of RNA metabolism such as other DEAD box family proteins which ensure accurate splicing and mRNA turnover. By affecting these pathways DDX53 can impact cellular growth control mechanisms.

DDX53's aberrant expression is linked to certain cancers most prominently in lung cancer and melanoma. Its expression correlates with tumor presence and progression. The interaction of DDX53 with testis-specific proteins in tumors makes it a relevant target for cancer immunotherapy. Furthermore its specificity to cancerous tissues over normal ones barring the testis positions DDX53 as a potentially valuable target for cancer vaccine development leveraging immune responses against tumor cells expressing this antigen.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDX53

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX53/CT26 antibody [EPR27384-107]

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Western blot - Anti-DDX53/CT26 antibody [EPR27384-107] (AB313333)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise