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AB76947

Anti-DDX54 antibody

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(4 Publications)

Rabbit Polyclonal DDX54 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human DDX54.

View Alternative Names

ATP-dependent RNA helicase DDX54, ATP-dependent RNA helicase DP97, DEAD box RNA helicase 97 kDa, DEAD box protein 54, DDX54

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX54 antibody (AB76947)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX54 antibody (AB76947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX54 with ab76947 at 1/200 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-DDX54 antibody (AB76947)
  • IP

Unknown

Immunoprecipitation - Anti-DDX54 antibody (AB76947)

ab76947 at 1µg/ml staining DDX54 in HeLa whole cell lysate immunoprecipitated using ab76947 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-DDX54 antibody (ab76947)

Predicted band size: 98 kDa

false

Western blot - Anti-DDX54 antibody (AB76947)
  • WB

Unknown

Western blot - Anti-DDX54 antibody (AB76947)

All lanes:

Western blot - Anti-DDX54 antibody (ab76947) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 98 kDa

Observed band size: 180 kDa,230 kDa,72 kDa,99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human DDX54. The exact immunogen used to generate this antibody is proprietary information.

Q8TDD1

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG2 antibody [EPR11910] (AB174850)

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Primary Antibodies

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Assay Kits

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab76947 was affinity purified using an epitope specific to DDX54 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX54 also known as DEAD Box Polypeptide 54 or DP97 is an ATP-dependent RNA helicase that belongs to the DEAD box family. It plays a mechanical role in the unwinding and remodeling of RNA secondary structures facilitating various RNA metabolic processes. This protein has a molecular mass of approximately 82 kDa and expresses widely in various tissues including the liver and brain. Its function is critical for maintaining normal cellular physiology by participating in processes like transcription regulation and ribosome biogenesis.
Biological function summary

DDX54 interacts with nuclear hormone receptors such as the Estrogen receptor to modulate gene expression. It acts as a coregulator in this context affecting transcriptional regulation and cellular response to hormones. This protein forms part of a multiprotein complex that functions in transcriptional machinery highlighting its integration into larger structural and functional frameworks within the cell. Through these interactions DDX54 significantly impacts cellular growth differentiation and response to external stimuli.

Pathways

DDX54 plays an important role in the steroid hormone receptor signaling pathway and the ribosome biogenesis pathway. It interacts with proteins including the Estrogen receptor and other coregulators influencing the expression of genes involved in cell proliferation and metabolism. These interactions position DDX54 as a critical node in cellular communication affecting pathways that manage cellular growth and maintenance.

DDX54 has associations with breast cancer and neurodegenerative diseases. In breast cancer it interacts with the Estrogen receptor influencing estrogen-responsive genes that can drive tumor progression. Its role in neurodegenerative diseases involves regulating stress response pathways where misregulated RNA processing might contribute to disease pathology. Connections to other proteins implicated in these diseases highlight its potential as a therapeutic target for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleolar RNA helicase that is involved in diverse cellular processes including ribosome biogenesis, DNA damage response, RNA splicing, transcriptional regulation, innate immunity, and nervous system development. Plays an essential role in the formation of the ribosome active site by remodeling rRNA structure and initiating peptidyl transferase center formation (PubMed : 38632236). Represses the transcriptional activity of several nuclear receptors. Regulates transcriptome dynamics during DNA damage response. Mechanistically, acts via increased interaction with a well-defined class of pre-mRNAs that contain introns with weak acceptor splice sites, as well as by protein-protein contacts within components of U2 snRNP and spliceosomal B complex. These activities lead to reduced intron retention and enhanced processing rates of its target transcripts (PubMed : 28596291). Acts as an inhibitor of type I interferon antiviral response by facilitating ALKBH5-mediated demethylation of specific transcripts (PubMed : 40793791). Contributes to central nervous system myelination, likely through regulation of oligodendrocyte differentiation and myelin sheath formation (By similarity).
See full target information DDX54
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cerebrovascular diseases (Basel, Switzerland) 52:306-317 PubMed36122568

2022

circCELF1 Induces the Apoptosis and Autophagy of Astrocytes in Ischemic Stroke via Upregulating NFAT5.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Junfang Teng

Frontiers in medicine 9:861371 PubMed35492354

2022

Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lujia Feng,Haichun Li,Yong Du,Ting Zhang,Yingting Zhu,Zhidong Li,Ling Zhao,Xing Wang,Gongpei Wang,Linbin Zhou,Zhaorong Jiang,Zheng Liu,Zhancong Ou,Yuwen Wen,Yehong Zhuo

Molecular therapy oncolytics 25:189-200 PubMed35592388

2022

LncRNA CBR3-AS1 promotes osteosarcoma progression through the network of miR-140-5p/DDX54-NUCKS1-mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weitao Yao,Jingyu Hou,Guoqing Liu,Fangxing Wu,Qiang Yan,Liangyu Guo,Chuchu Wang

Journal of translational medicine 19:333 PubMed34353336

2021

MYBL2-induced PITPNA-AS1 upregulates SIK2 to exert oncogenic function in triple-negative breast cancer through miR-520d-5p and DDX54.

Applications

Unspecified application

Species

Unspecified reactive species

Bolong Liu,Pingbo Yao,Feng Xiao,Jianjin Guo,Lianghui Wu,Yong Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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