AB109591

Anti-DDX55 antibody

Name: Anti-DDX55 antibody (ab109591) | Abcam
Brand: Abcam Limited
SKU: AB109591
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal DDX55 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DDX55 aa 150-250.

View Alternative Names

KIAA1595, DDX55, ATP-dependent RNA helicase DDX55, DEAD box protein 55

2 Images

Unknown

Immunoprecipitation - Anti-DDX55 antibody (AB109591)

Detection of DDX55 by Western Blot of Immunprecipitate.
ab109591 at 0.4µg/ml staining DDX55 in HeLa whole cell lysate immunoprecipitated using ab109591 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DDX55 antibody (ab109591)

Predicted band size: 69 kDa

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Unknown

Western blot - Anti-DDX55 antibody (AB109591)

All lanes:

Western blot - Anti-DDX55 antibody (ab109591) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 69 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human DDX55 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q8NHQ9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX55 also known as DEAD box protein 55 is a member of the DEAD box protein family. Mechanically it acts as an RNA helicase involved in altering RNA secondary structure. This function plays a role in RNA processing events including transcription splicing and ribosome assembly. DDX55 has a mass of approximately 67 kDa and expresses in various tissues indicating its broad functional importance in cellular biology.

Biological function summary

Many RNA processing activities depend on RNA helicases like DDX55. It influences the regulation of gene expression by participating in RNP complex remodeling. It interacts dynamically with other molecules during RNA metabolic processes although not strictly part of a single complex. Its activity affects cellular growth and maintenance showing its involvement in essential biological processes.

Pathways

DDX55 engages in important cellular processes such as RNA splicing and translation initiation. It associates with pathways regulating gene expression at the post-transcriptional level. DDX55 shows an interaction with proteins like eIF4A a vital component of the translation initiation complex influencing the initiation steps of protein synthesis. This relationship underlines its involvement in mRNA lifecycle management.

Errors in DDX55 function or expression can relate to conditions like cancer and neurodegenerative diseases. Its role in gene expression links it to the abnormal cellular proliferation seen in some cancers. Additionally DDX55 interacts with proteins like p53 an important regulator of the cell cycle and apoptosis. Disruptions in these interactions may contribute to the development of disease by affecting cell survival pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable ATP-binding RNA helicase.

See full target information DDX55

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicine & pharmacotherapy = Biomedecine & phar 103:9-17 PubMed29635133

2018

Icariside II alleviates oxygen-glucose deprivation and reoxygenation-induced PC12 cell oxidative injury by activating Nrf2/SIRT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Linying Feng,Jianmei Gao,Yuangui Liu,Jingshan Shi,Qihai Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX55 antibody

Immunoprecipitation - Anti-DDX55 antibody (AB109591)

Western blot - Anti-DDX55 antibody (AB109591)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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