AB115178

Anti-DDX56/DDX21 antibody

Name: Anti-DDX56/DDX21 antibody (ab115178) | Abcam
Brand: Abcam Limited
SKU: AB115178
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal DDX56/DDX21 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DDX56.

View Alternative Names

DDX21, NOH61, DDX56, Probable ATP-dependent RNA helicase DDX56, ATP-dependent 61 kDa nucleolar RNA helicase, DEAD box protein 21, DEAD box protein 56

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB115178)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB115178)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56/DDX21 with ab115178 at 1/1000 (1μg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-DDX56/DDX21 antibody (AB115178)

Detection of DDX56/DDX21 by Immunoprecipitation analysis in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) using ab115178 for IP at 3 ug/mg lysate. Subsequence WB detection was performed using 1 ug/ml ab115178.

All lanes:

Immunoprecipitation - Anti-DDX56/DDX21 antibody (ab115178)

Predicted band size: 61 kDa

false

Unknown

Western blot - Anti-DDX56/DDX21 antibody (AB115178)

All lanes:

Western blot - Anti-DDX56/DDX21 antibody (ab115178) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 61 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human DDX56. The exact immunogen used to generate this antibody is proprietary information.

Q9NY93

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX56 also known as DDX21 is a protein that is part of the DExD/H-box family of RNA helicases. This protein has an approximate mass of 87 kDa and functions in the unwinding of RNA carrying out ATP-dependent helicase activity. DDX56 is widely expressed in human cells with a particular abundance in the nucleolus where it plays an important role in the formation and processing of ribosomal RNA.

Biological function summary

DDX56 acts in the regulation of ribosome assembly and ribosomal RNA processing. This protein is involved in the transcription and maturation of pre-rRNA an important step in ribosome biogenesis. DDX56 forms part of the small subunit (SSU) processome complex which contains various proteins necessary for early stages of ribosome assembly. The SSU processome is essential for the processing of primary rRNA transcripts into 18S rRNA.

Pathways

DDX56 is important in ribosome biogenesis and RNA transcription. It interacts with components of the RNA polymerase I pathway which is responsible for the transcription of rRNA genes. DDX56 also associates with the snoRNP complexes involved in the modification and processing of rRNA. Its activity affects proteins like fibrillarin and U3 snoRNA which are required for rRNA processing and ribosome assembly.

Researchers have linked DDX56 to certain cancers such as breast cancer and prostate cancer. In these conditions DDX56 often shows altered expression which impacts ribosome biogenesis and cellular proliferation. The protein interacts with nucleolin another nucleolar protein which modulates rRNA synthesis. This relationship plays a role in the progression of cancer making DDX56 a potential target for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nucleolar RNA helicase that plays a role in various biological processes including innate immunity, ribosome biogenesis or nucleolus organization (PubMed : 31340999, PubMed : 33789112). Plays an essential role in maintaining nucleolar integrity in planarian stem cells (PubMed : 33789112). Maintains embryonic stem cells proliferation by conventional regulation of ribosome assembly and interaction with OCT4 and POU5F1 complex (By similarity). Regulates antiviral innate immunity by inhibiting the virus-triggered signaling nuclear translocation of IRF3 (PubMed : 31340999). Mechanistically, acts by disrupting the interaction between IRF3 and importin IPO5 (PubMed : 31340999). May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.. (Microbial infection) Helicase activity is important for packaging viral RNA into virions during West Nile virus infection.. (Microbial infection) Plays a positive role in foot-and-mouth disease virus replication by inhibiting the phosphorylation of IRF3 leading to inhibition of type I interferon.. (Microbial infection) Plays a positive role in EMCV replication by interrupting IRF3 phosphorylation and its nucleus translocation.

See full target information DDX56

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:12647-12658 PubMed34903139

2021

Downregulation of DEAD-box helicase 21 (DDX21) inhibits proliferation, cell cycle, and tumor growth in colorectal cancer via targeting cell division cycle 5-like (CDC5L).

Applications

Unspecified application

Species

Unspecified reactive species

Kai Wang,Baosong Li,Peng Fan,Xiang Ren,Hong Jiang

Stem cells (Dayton, Ohio) 38:556-573 PubMed31721359

2020

A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Junyao Yang,Ana Moraga,Jing Xu,Yue Zhao,Peiyi Luo,Ka Hou Lao,Andriana Margariti,Qiang Zhao,Wei Ding,Gang Wang,Min Zhang,Lei Zheng,Zhongyi Zhang,Yanhua Hu,Wen Wang,Lisong Shen,Alberto Smith,Ajay M Shah,Qian Wang,Lingfang Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX56/DDX21 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB115178)

Immunoprecipitation - Anti-DDX56/DDX21 antibody (AB115178)

Western blot - Anti-DDX56/DDX21 antibody (AB115178)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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