Rabbit Polyclonal DDX56/DDX21 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX56 aa 250-450.
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Publications
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- Journal of cell science 133:2019DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Li,Shaozu Fu,Zhengqian Wu,Wenping Yang,Yi Ru,Hongbing Shu,Xiangtao Liu,Haixue ZhengPubMed 31340999
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DDX56 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY93
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Nucleolar RNA helicase that plays a role in various biological processes including innate immunity, ribosome biogenesis or nucleolus organization (PubMed:31340999, PubMed:33789112). Plays an essential role in maintaining nucleolar integrity in planarian stem cells (PubMed:33789112). Maintains embryonic stem cells proliferation by conventional regulation of ribosome assembly and interaction with OCT4 and POU5F1 complex (By similarity). Regulates antiviral innate immunity by inhibiting the virus-triggered signaling nuclear translocation of IRF3 (PubMed:31340999). Mechanistically, acts by disrupting the interaction between IRF3 and importin IPO5 (PubMed:31340999). May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity. (Microbial infection) Helicase activity is important for packaging viral RNA into virions during West Nile virus infection. (Microbial infection) Plays a positive role in foot-and-mouth disease virus replication by inhibiting the phosphorylation of IRF3 leading to inhibition of type I interferon. (Microbial infection) Plays a positive role in EMCV replication by interrupting IRF3 phosphorylation and its nucleus translocation.
Alternative names
DDX21, NOH61, DDX56, Probable ATP-dependent RNA helicase DDX56, ATP-dependent 61 kDa nucleolar RNA helicase, DEAD box protein 21, DEAD box protein 56
Recommended products
Rabbit Polyclonal DDX56/DDX21 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX56 aa 250-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DDX56 aa 250-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NY93
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
DDX56 also known as DDX21 is a protein that is part of the DExD/H-box family of RNA helicases. This protein has an approximate mass of 87 kDa and functions in the unwinding of RNA carrying out ATP-dependent helicase activity. DDX56 is widely expressed in human cells with a particular abundance in the nucleolus where it plays an important role in the formation and processing of ribosomal RNA.
- Biological function summary
DDX56 acts in the regulation of ribosome assembly and ribosomal RNA processing. This protein is involved in the transcription and maturation of pre-rRNA an important step in ribosome biogenesis. DDX56 forms part of the small subunit (SSU) processome complex which contains various proteins necessary for early stages of ribosome assembly. The SSU processome is essential for the processing of primary rRNA transcripts into 18S rRNA.
- Pathways
DDX56 is important in ribosome biogenesis and RNA transcription. It interacts with components of the RNA polymerase I pathway which is responsible for the transcription of rRNA genes. DDX56 also associates with the snoRNP complexes involved in the modification and processing of rRNA. Its activity affects proteins like fibrillarin and U3 snoRNA which are required for rRNA processing and ribosome assembly.
- Associated diseases and disorders
Researchers have linked DDX56 to certain cancers such as breast cancer and prostate cancer. In these conditions DDX56 often shows altered expression which impacts ribosome biogenesis and cellular proliferation. The protein interacts with nucleolin another nucleolar protein which modulates rRNA synthesis. This relationship plays a role in the progression of cancer making DDX56 a potential target for therapeutic intervention in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-DDX56/DDX21 antibody (ab97648)
Paraformaldehyde-fixed HeLa cells stained for DDX56/DDX21 using ab97648 at 1/500 dilution in ICC/IF (green). Alpha tubulin filaments are detected with an ant-tubulin antibody (red).
Western blot - Anti-DDX56/DDX21 antibody (ab97648)
7.5% SDS PAGE
All lanes: Western blot - Anti-DDX56/DDX21 antibody (ab97648) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DDX56/DDX21 antibody (ab97648)
ab97648, at a 1/200 dilution, staining DDX56/DDX21 in paraformaldehyde fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (ab97648)
ab97648, at a 1/500 dilution, staining DDX56/DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.
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