AB97648

Anti-DDX56/DDX21 antibody

Name: Anti-DDX56/DDX21 antibody (ab97648) | Abcam
Brand: Abcam Limited
SKU: AB97648
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal DDX56/DDX21 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDX56 aa 250-450.

View Alternative Names

DDX21, NOH61, DDX56, Probable ATP-dependent RNA helicase DDX56, ATP-dependent 61 kDa nucleolar RNA helicase, DEAD box protein 21, DEAD box protein 56

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB97648)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB97648)

ab97648, at a 1/500 dilution, staining DDX56/DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-DDX56/DDX21 antibody (AB97648)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX56/DDX21 antibody (AB97648)

Paraformaldehyde-fixed HeLa cells stained for DDX56/DDX21 using ab97648 at 1/500 dilution in ICC/IF (green). Alpha tubulin filaments are detected with an ant-tubulin antibody (red).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB97648)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labelling DDX56 protein at nucleus with ab97648 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Unknown

Western blot - Anti-DDX56/DDX21 antibody (AB97648)

7.5% SDS PAGE

All lanes:

Western blot - Anti-DDX56/DDX21 antibody (ab97648) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DDX56 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q9NY93

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX56 also known as DDX21 is a protein that is part of the DExD/H-box family of RNA helicases. This protein has an approximate mass of 87 kDa and functions in the unwinding of RNA carrying out ATP-dependent helicase activity. DDX56 is widely expressed in human cells with a particular abundance in the nucleolus where it plays an important role in the formation and processing of ribosomal RNA.

Biological function summary

DDX56 acts in the regulation of ribosome assembly and ribosomal RNA processing. This protein is involved in the transcription and maturation of pre-rRNA an important step in ribosome biogenesis. DDX56 forms part of the small subunit (SSU) processome complex which contains various proteins necessary for early stages of ribosome assembly. The SSU processome is essential for the processing of primary rRNA transcripts into 18S rRNA.

Pathways

DDX56 is important in ribosome biogenesis and RNA transcription. It interacts with components of the RNA polymerase I pathway which is responsible for the transcription of rRNA genes. DDX56 also associates with the snoRNP complexes involved in the modification and processing of rRNA. Its activity affects proteins like fibrillarin and U3 snoRNA which are required for rRNA processing and ribosome assembly.

Researchers have linked DDX56 to certain cancers such as breast cancer and prostate cancer. In these conditions DDX56 often shows altered expression which impacts ribosome biogenesis and cellular proliferation. The protein interacts with nucleolin another nucleolar protein which modulates rRNA synthesis. This relationship plays a role in the progression of cancer making DDX56 a potential target for therapeutic intervention in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nucleolar RNA helicase that plays a role in various biological processes including innate immunity, ribosome biogenesis or nucleolus organization (PubMed : 31340999, PubMed : 33789112). Plays an essential role in maintaining nucleolar integrity in planarian stem cells (PubMed : 33789112). Maintains embryonic stem cells proliferation by conventional regulation of ribosome assembly and interaction with OCT4 and POU5F1 complex (By similarity). Regulates antiviral innate immunity by inhibiting the virus-triggered signaling nuclear translocation of IRF3 (PubMed : 31340999). Mechanistically, acts by disrupting the interaction between IRF3 and importin IPO5 (PubMed : 31340999). May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.. (Microbial infection) Helicase activity is important for packaging viral RNA into virions during West Nile virus infection.. (Microbial infection) Plays a positive role in foot-and-mouth disease virus replication by inhibiting the phosphorylation of IRF3 leading to inhibition of type I interferon.. (Microbial infection) Plays a positive role in EMCV replication by interrupting IRF3 phosphorylation and its nucleus translocation.

See full target information DDX56

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109827 PubMed38827395

2024

DDX56 promotes EMT and cancer stemness via MELK-FOXM1 axis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Tianyi Wang,Ximin Wang,XinYu Ge,Tao Yang,Wei Wang

Medical science monitor : international medical journal of experimental and clinical research 26:e922090 PubMed32489187

2020

Absence of Long Noncoding RNA H19 Promotes Childhood Nephrotic Syndrome through Inhibiting ADCK4 Signal.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwen Xu,Tingting Ge,Hongxia Zhou,Lin Zhang,Liping Zhao

Journal of cell science 133: PubMed31340999

2019

DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Shaozu Fu,Zhengqian Wu,Wenping Yang,Yi Ru,Hongbing Shu,Xiangtao Liu,Haixue Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX56/DDX21 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB97648)

Immunocytochemistry/ Immunofluorescence - Anti-DDX56/DDX21 antibody (AB97648)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (AB97648)

Western blot - Anti-DDX56/DDX21 antibody (AB97648)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise