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AB229206

Anti-DDX59 antibody

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(2 Publications)

Rabbit Polyclonal DDX59 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDX59.

View Alternative Names

ZNHIT5, DDX59, Probable ATP-dependent RNA helicase DDX59, DEAD box protein 59, Zinc finger HIT domain-containing protein 5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX59 antibody (AB229206)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX59 antibody (AB229206)

Paraffin-embedded human breast cancer tissue stained for DDX59 using ab229206 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-DDX59 antibody (AB229206)
  • WB

Supplier Data

Western blot - Anti-DDX59 antibody (AB229206)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-DDX59 antibody (ab229206) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 69 kDa

false

Western blot - Anti-DDX59 antibody (AB229206)
  • WB

Supplier Data

Western blot - Anti-DDX59 antibody (AB229206)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-DDX59 antibody (ab229206) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DDX59. The exact immunogen used to generate this antibody is proprietary information.

Q5T1V6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX59 also known as DEAD box protein 59 is an RNA helicase with an approximate mass of 66 kDa. This protein unwinds RNA duplexes through ATP-dependent mechanism enabling the processing and remodeling of RNA molecules. DDX59 shows expression in various tissues with higher levels observed in the brain and heart indicating its potential importance in these organs.
Biological function summary

DEAD box protein 59 plays a role in RNA metabolism influencing splicing ribosome biogenesis and RNA degradation. DDX59 may function as part of a multi-protein complex working closely with other RNA-binding proteins to regulate RNA structure and function. This regulation is important for gene expression control and proper cell function.

Pathways

DDX59 integrates in critical processes like the RNA splicing pathway and mRNA surveillance pathway. It often collaborates with proteins such as SF3B1 in RNA splicing ensuring the accuracy of mRNA processing. This interaction helps maintain cellular homeostasis and protect against errors in gene expression that might lead to cellular distress or dysfunction.

DDX59 has connections to orofaciodigital syndrome type I and neural tube defects. Changes in DDX59 function or expression levels might contribute to these conditions making it a potential biomarker or target for therapeutic interventions. The protein links with other players like DYRK1A in these disorders together affecting neurodevelopment and craniofacial structures.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information DDX59
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:745-758 PubMed40164685

2025

Human neuron chimeric mice reveal impairment of DVL-1-mediated neuronal migration by sevoflurane and potential treatment by rTMS.

Applications

Unspecified application

Species

Unspecified reactive species

Youyi Zhao,Ya Zhao,Lirong Liang,Andi Chen,Yuqian Li,Ke Liu,Rougang Xie,Honghui Mao,Boyang Ren,Bosong Huang,Changhong Shi,Zhicheng Shao,Shengxi Wu,Yazhou Wang,Hui Zhang

Journal of cellular biochemistry 120:17293-17302 PubMed31111544

2019

MicroRNA-628-5p inhibits cell proliferation in glioma by targeting DDX59.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xie,Yan Wang,Yuanmei Liao,Qiu Han,Zhichao Qiu,Yinbing Chen,Xiaohua Zuo
View all publications
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