AB151984

Anti-DDX6 antibody

Name: Anti-DDX6 antibody (ab151984) | Abcam
Brand: Abcam Limited
SKU: AB151984
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal DDX6 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DDX6 aa 1-250.

View Alternative Names

HLR2, RCK, DDX6, Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-DDX6 antibody (AB151984)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DDX6 antibody (AB151984)

HeLa cells stained for DDX6 (green) using ab151984 at 1/500 dilution in ICC/IF.

Unknown

Western blot - Anti-DDX6 antibody (AB151984)

10% SDS PAGE

All lanes:

Western blot - Anti-DDX6 antibody (ab151984) at 1/1000 dilution

All lanes:

Hela whole cell lysate at 30 µg

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DDX6 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P26196

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX6 also known as RCK or p54 is an RNA helicase with a molecular mass of approximately 54 kDa. This protein is primarily located in the cytoplasm and is involved in processes such as mRNA degradation and translational repression. DDX6 acts mechanically by unwinding RNA molecules which facilitates various RNA processing events critical for cellular function. Its expression occurs broadly across different tissues indicating its significant role in diverse cellular operations.

Biological function summary

DDX6 functions as part of the RNA-induced silencing complex (RISC) and is involved in messenger ribonucleoprotein (mRNP) granule formation. These granules include P-bodies and stress granules which are essential for mRNA metabolism and storage. DDX6 collaborates with other proteins such as EDC3 and DCP1 to regulate mRNA turnover and decay. Its role in post-transcriptional control impacts gene expression regulation at multiple levels.

Pathways

The involvement of DDX6 in the RNA interference (RNAi) and nonsense-mediated mRNA decay (NMD) pathways is significant. In the RNAi pathway it interacts with proteins like AGO2 to mediate gene silencing by microRNAs. DDX6 also participates in the NMD pathway by targeting aberrant mRNAs for degradation assisting proteins like UPF1. These pathways demonstrate the important role of DDX6 in maintaining cellular RNA homeostasis and preventing the accumulation of defective RNAs.

DDX6 has been linked to cancer and neurodegenerative diseases. Its interaction with cancer-related proteins like AURKA suggests a role in tumorigenesis where it may affect cell proliferation and survival. Additionally DDX6's involvement in neurodegenerative disorders has been observed where dysregulation of its associated RNA processes may contribute to disease pathology. Understanding these connections provides insight into potential therapeutic targets for related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed : 25995375, PubMed : 27342281, PubMed : 31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed : 27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed : 16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed : 26098573).

See full target information DDX6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:113413 PubMed38096059

2023

Synonymous codon usage regulates translation initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Chloe L Barrington,Gabriel Galindo,Amanda L Koch,Emma R Horton,Evan J Morrison,Samantha Tisa,Timothy J Stasevich,Olivia S Rissland

Foods (Basel, Switzerland) 11: PubMed35954013

2022

Shrinkage Properties and Their Relationship with Degradation of Proteins Linking the Endomysium and Myofibril in Lamb Meat Submitted to Heating or Air Drying.

Applications

Unspecified application

Species

Unspecified reactive species

Weili Rao,Zhenxiao Shi,Sijia Liu,Ying Shu,Xiaoyu Chai,Zhisheng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-DDX6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DDX6 antibody (AB151984)

Western blot - Anti-DDX6 antibody (AB151984)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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