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Rabbit Polyclonal DDX6 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human DDX6 aa 1-250.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (AB151984), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DDX6 antibody (AB151984), expandable thumbnail
  • Western blot - Anti-DDX6 antibody (AB151984), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (AB151984), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human DDX6 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P26196

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Chicken
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Cow, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chicken, Cow, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Predicted
Predicted

Species

Chicken, Cow, Xenopus laevis, Xenopus tropicalis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25995375, PubMed:27342281, PubMed:31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed:27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed:16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573).

Alternative names

Recommended products

Rabbit Polyclonal DDX6 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human DDX6 aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human DDX6 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P26196
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

DDX6 also known as RCK or p54 is an RNA helicase with a molecular mass of approximately 54 kDa. This protein is primarily located in the cytoplasm and is involved in processes such as mRNA degradation and translational repression. DDX6 acts mechanically by unwinding RNA molecules which facilitates various RNA processing events critical for cellular function. Its expression occurs broadly across different tissues indicating its significant role in diverse cellular operations.

Biological function summary

DDX6 functions as part of the RNA-induced silencing complex (RISC) and is involved in messenger ribonucleoprotein (mRNP) granule formation. These granules include P-bodies and stress granules which are essential for mRNA metabolism and storage. DDX6 collaborates with other proteins such as EDC3 and DCP1 to regulate mRNA turnover and decay. Its role in post-transcriptional control impacts gene expression regulation at multiple levels.

Pathways

The involvement of DDX6 in the RNA interference (RNAi) and nonsense-mediated mRNA decay (NMD) pathways is significant. In the RNAi pathway it interacts with proteins like AGO2 to mediate gene silencing by microRNAs. DDX6 also participates in the NMD pathway by targeting aberrant mRNAs for degradation assisting proteins like UPF1. These pathways demonstrate the important role of DDX6 in maintaining cellular RNA homeostasis and preventing the accumulation of defective RNAs.

Associated diseases and disorders

DDX6 has been linked to cancer and neurodegenerative diseases. Its interaction with cancer-related proteins like AURKA suggests a role in tumorigenesis where it may affect cell proliferation and survival. Additionally DDX6's involvement in neurodegenerative disorders has been observed where dysregulation of its associated RNA processes may contribute to disease pathology. Understanding these connections provides insight into potential therapeutic targets for related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (ab151984), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (ab151984)

    Paraffin embedded mouse stomach tissue stained for DDX6 using ab151984 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-DDX6 antibody (ab151984), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DDX6 antibody (ab151984)

    HeLa cells stained for DDX6 (green) using ab151984 at 1/500 dilution in ICC/IF.

  • Western blot - Anti-DDX6 antibody (ab151984), expandable thumbnail

    Western blot - Anti-DDX6 antibody (ab151984)

    10% SDS PAGE

    All lanes: Western blot - Anti-DDX6 antibody (ab151984) at 1/1000 dilution

    All lanes: Hela whole cell lysate at 30 µg

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (ab151984), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody (ab151984)

    Immunohistochemical analysis of paraffin embedded Ca922 (Human gingival carcinoma) xenograft tissue labeling DDX6 with ab151984 at 1/100.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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