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AB174277

Anti-DDX6 antibody [EPR12146]

5

(1 Review)

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(5 Publications )

Rabbit Recombinant Monoclonal DDX6 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR12146] (AB174277)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR12146] (AB174277)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling DDX6 with ab174277 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-DDX6 antibody [EPR12146] (AB174277)
  • WB

Supplier Data

Western blot - Anti-DDX6 antibody [EPR12146] (AB174277)

All lanes:

Western blot - Anti-DDX6 antibody [EPR12146] (ab174277) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Western blot - Anti-DDX6 antibody [EPR12146] (AB174277)
  • WB

Lab

Western blot - Anti-DDX6 antibody [EPR12146] (AB174277)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : DDX6 knockout HAP1 cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lane 4 : HeLa cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab174277 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab174277 was shown to recognize DDX6 when DDX6 knockout samples were used, along with additional cross-reactive bands. Wild-type and DDX6 knockout samples were subjected to SDS-PAGE. ab174277 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-DDX6 antibody [EPR12146] (ab174277)

Predicted band size: 54 kDa

false

  • Carrier free

    Anti-DDX6 antibody [EPR12146] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12146

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX6 also known as RCK or p54 is an RNA helicase with a molecular mass of approximately 54 kDa. This protein is primarily located in the cytoplasm and is involved in processes such as mRNA degradation and translational repression. DDX6 acts mechanically by unwinding RNA molecules which facilitates various RNA processing events critical for cellular function. Its expression occurs broadly across different tissues indicating its significant role in diverse cellular operations.
Biological function summary

DDX6 functions as part of the RNA-induced silencing complex (RISC) and is involved in messenger ribonucleoprotein (mRNP) granule formation. These granules include P-bodies and stress granules which are essential for mRNA metabolism and storage. DDX6 collaborates with other proteins such as EDC3 and DCP1 to regulate mRNA turnover and decay. Its role in post-transcriptional control impacts gene expression regulation at multiple levels.

Pathways

The involvement of DDX6 in the RNA interference (RNAi) and nonsense-mediated mRNA decay (NMD) pathways is significant. In the RNAi pathway it interacts with proteins like AGO2 to mediate gene silencing by microRNAs. DDX6 also participates in the NMD pathway by targeting aberrant mRNAs for degradation assisting proteins like UPF1. These pathways demonstrate the important role of DDX6 in maintaining cellular RNA homeostasis and preventing the accumulation of defective RNAs.

DDX6 has been linked to cancer and neurodegenerative diseases. Its interaction with cancer-related proteins like AURKA suggests a role in tumorigenesis where it may affect cell proliferation and survival. Additionally DDX6's involvement in neurodegenerative disorders has been observed where dysregulation of its associated RNA processes may contribute to disease pathology. Understanding these connections provides insight into potential therapeutic targets for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed : 25995375, PubMed : 27342281, PubMed : 31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed : 27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed : 16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed : 26098573).
See full target information DDX6

Alternative Names

HLR2, RCK, DDX6, Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8051 PubMed40877279

2025

Translational repression by 4E-T is crucial to maintain the prophase-I arrest in vertebrate oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Heim,Shiya Cheng,Jan Orth,Florian Stengel,Melina Schuh,Thomas U Mayer

Nature communications 15:10044 PubMed39567493

2024

Two mechanisms repress cyclin B1 translation to maintain prophase arrest in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shiya Cheng,Melina Schuh

Nature microbiology 9:1256-1270 PubMed38649412

2024

Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yinggui Yang,Tengteng Ding,Ying Cong,Xiaomin Luo,Changlin Liu,Ting Gong,Min Zhao,Xichun Zheng,Chenglin Li,Yuanbin Zhang,Jiayi Zhou,Chuping Ni,Xueyu Zhang,Ziliang Ji,Tao Wu,Shaodong Yang,Qingchun Zhou,Dinglan Wu,Xinqi Gong,Qingyou Zheng,Xin Li

Cell genomics 3:100303 PubMed37228754

2023

exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Emily L LaPlante,Alessandra Stürchler,Robert Fullem,David Chen,Anne C Starner,Emmanuel Esquivel,Eric Alsop,Andrew R Jackson,Ionita Ghiran,Getulio Pereira,Joel Rozowsky,Justin Chang,Mark B Gerstein,Roger P Alexander,Matthew E Roth,Jeffrey L Franklin,Robert J Coffey,Robert L Raffai,Isabelle M Mansuy,Stavros Stavrakis,Andrew J deMello,Louise C Laurent,Yi-Ting Wang,Chia-Feng Tsai,Tao Liu,Jennifer Jones,Kendall Van Keuren-Jensen,Eric Van Nostrand,Bogdan Mateescu,Aleksandar Milosavljevic

Nature chemical biology 13:174-180 PubMed27918561

2016

A human microprotein that interacts with the mRNA decapping complex.

Applications

Unspecified application

Species

Unspecified reactive species

Nadia G D'Lima,Jiao Ma,Lauren Winkler,Qian Chu,Ken H Loh,Elizabeth O Corpuz,Bogdan A Budnik,Jens Lykke-Andersen,Alan Saghatelian,Sarah A Slavoff
View all publications
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Product promise

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