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AB249788

Anti-DDX6 antibody [EPR12146] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal DDX6 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HLR2, RCK, DDX6, Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR12146] - BSA and Azide free (AB249788)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX6 antibody [EPR12146] - BSA and Azide free (AB249788)

This data was developed using ab174277, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling DDX6 with ab174277 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-DDX6 antibody [EPR12146] - BSA and Azide free (AB249788)
  • WB

Supplier Data

Western blot - Anti-DDX6 antibody [EPR12146] - BSA and Azide free (AB249788)

This data was developed using ab174277, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DDX6 antibody [EPR12146] (<a href='/en-us/products/primary-antibodies/ddx6-antibody-epr12146-ab174277'>ab174277</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 54 kDa

Observed band size: 54 kDa

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Western blot - Anti-DDX6 antibody [EPR12146] - BSA and Azide free (AB249788)
  • WB

Lab

Western blot - Anti-DDX6 antibody [EPR12146] - BSA and Azide free (AB249788)

This data was developed using ab174277, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : DDX6 knockout HAP1 cell lysate (20 μg)

Lane 3 : HepG2 cell lysate (20 μg)

Lane 4 : HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab174277 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab174277 was shown to recognize DDX6 when DDX6 knockout samples were used, along with additional cross-reactive bands. Wild-type and DDX6 knockout samples were subjected to SDS-PAGE. ab174277 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-DDX6 antibody [EPR12146] (<a href='/en-us/products/primary-antibodies/ddx6-antibody-epr12146-ab174277'>ab174277</a>)

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12146

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249788 is the carrier-free version of ab174277.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX6 also known as RCK or p54 is an RNA helicase with a molecular mass of approximately 54 kDa. This protein is primarily located in the cytoplasm and is involved in processes such as mRNA degradation and translational repression. DDX6 acts mechanically by unwinding RNA molecules which facilitates various RNA processing events critical for cellular function. Its expression occurs broadly across different tissues indicating its significant role in diverse cellular operations.
Biological function summary

DDX6 functions as part of the RNA-induced silencing complex (RISC) and is involved in messenger ribonucleoprotein (mRNP) granule formation. These granules include P-bodies and stress granules which are essential for mRNA metabolism and storage. DDX6 collaborates with other proteins such as EDC3 and DCP1 to regulate mRNA turnover and decay. Its role in post-transcriptional control impacts gene expression regulation at multiple levels.

Pathways

The involvement of DDX6 in the RNA interference (RNAi) and nonsense-mediated mRNA decay (NMD) pathways is significant. In the RNAi pathway it interacts with proteins like AGO2 to mediate gene silencing by microRNAs. DDX6 also participates in the NMD pathway by targeting aberrant mRNAs for degradation assisting proteins like UPF1. These pathways demonstrate the important role of DDX6 in maintaining cellular RNA homeostasis and preventing the accumulation of defective RNAs.

DDX6 has been linked to cancer and neurodegenerative diseases. Its interaction with cancer-related proteins like AURKA suggests a role in tumorigenesis where it may affect cell proliferation and survival. Additionally DDX6's involvement in neurodegenerative disorders has been observed where dysregulation of its associated RNA processes may contribute to disease pathology. Understanding these connections provides insight into potential therapeutic targets for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed : 25995375, PubMed : 27342281, PubMed : 31422817). Plays a role in P-bodies to coordinate the storage of translationally inactive mRNAs in the cytoplasm and prevent their degradation (PubMed : 27342281). In the process of mRNA degradation, plays a role in mRNA decapping (PubMed : 16364915). Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed : 26098573).
See full target information DDX6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

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