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AB151217

Anti-DDX60 antibody

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(1 Publication)

Rabbit Polyclonal DDX60 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDX60 aa 1150-1250.

View Alternative Names

Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60, DDX60

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX60 antibody (AB151217)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX60 antibody (AB151217)

Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling DDX60 with ab151217 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-DDX60 antibody (AB151217)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DDX60 antibody (AB151217)

Immunofluorescent staining of Human cell line A-431 shows positivity in cytoskeleton (intermediate filaments). Recommended concentration of ab151217 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DDX60 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information.

Q8IY21

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDX60 also known as DExD/H-box helicase 60 is a protein involved in antiviral responses. It has a molecular mass of approximately 180 kDa. DDX60 acts as an ATP-dependent RNA helicase unwinding RNA structures during viral replication processes. This protein is expressed mainly in immune-related tissues like the spleen and peripheral blood leukocytes indicating its role in immunity. Alternate names include DDX60L which denotes a related protein with similar functions.
Biological function summary

DDX60 plays an essential role in the detection of viral RNA acting as a sensor in the innate immune system. It forms part of a complex involved in recognizing and responding to viral infections. DDX60 interacts with other proteins like RIG-I a pivotal component in initiating antiviral signaling cascades. Through these interactions DDX60 facilitates the production of interferons which are key elements in mounting an effective immune response against viruses.

Pathways

DDX60 functions within the RIG-I-like receptor signaling pathway an important antiviral defense mechanism. This pathway includes proteins such as MAVS and IRF3 which work together to stimulate the expression of cytokines following pathogen recognition. DDX60's activity enhances the sensitivity of this pathway boosting the host's capacity to control viral replication and spread contributing to the overall immune response.

Variations in DDX60 expression have been linked to autoimmune conditions like systemic lupus erythematosus (SLE) and chronic viral infections such as hepatitis C. Abnormal regulation of this protein can lead to inappropriate immune activation or impaired response to infections. DDX60's association with RIG-I suggests its involvement in these diseases as both proteins are important in the body's effort to manage viral threats and the maintenance of immune homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Positively regulates RIGI- and IFIH1/MDA5-dependent type I interferon and interferon inducible gene expression in response to viral infection. Binds ssRNA, dsRNA and dsDNA and can promote the binding of RIGI to dsRNA. Exhibits antiviral activity against hepatitis C virus and vesicular stomatitis virus (VSV).
See full target information DDX60
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed39861799

2025

Exploration of the Role of Cyclophilins in Established Hepatitis B and C Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Molle,Sarah Duponchel,Jennifer Rieusset,Michel Ovize,Alexander V Ivanov,Fabien Zoulim,Birke Bartosch
View all publications
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Product promise

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