AB195173

Anti-Deadpan antibody [11D1BC7]

Name: Anti-Deadpan antibody [11D1BC7] (ab195173) | Abcam
Brand: Abcam Limited
SKU: AB195173
Price: 170 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(37 Publications)

Anti-Deadpan antibody [11D1BC7] (ab195173) is a rat monoclonal antibody detecting Deadpan. Suitable for Drosophila melanogaster.

- Over 20 publications

View Alternative Names

CG8704, dpn, Protein deadpan

2 Images

Supplier Data

Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173)

Immunohistochemistry analysis using ab195173 detecting the nuclear transcription factor Deadpan protein in the neuroblasts of the third instar larval brain of Drosophila melanogaster. The brain was fixed in 4% formaldehyde (20 min) and then incubated in 1%BSA / 10% normal goat serum in 0.1% PBS-Tween for 1hr to permeabilise the cells and block non-specific protein-protein interactions. The brain was then incubated with ab195173 (10 μg/mL) overnight at +4°C and detected with Alexa Fluor^® 555 goat anti-rat IgG. The counterstain was chicken anti-GFP detected with Alexa Fluor^® 488 used according to manufacturer's instructions.

Supplier Data

Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173)

Immunohistochemistry analysis using ab195173 detecting Drosophila stage 11 embryos stained showing Deadpan (Dpn) protein in all neuroblast nuclei. The Deadpan stain used ab195173 at 10 μg/mL and detected with Alexa Fluor^® 405 conjugated goat anti-rat (at 10μg/mL).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

11D1BC7

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Drosophila melanogaster

Applications

IHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "IHC-species-checked": "testedAndGuaranteed", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Deadpan antibody [11D1BC7] (ab195173) is a rat monoclonal antibody Trusted by the scientific community
Anti-Deadpan [11D1BC7] (ab195173) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification notes

The purity of ab195173 is near homogeneity, as judged by SDS-PAGE. ab195173 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.>95% based on SDS-PAGE.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor of genes that require a bHLH protein for their transcription (PubMed : 1427077, PubMed : 24618901, PubMed : 28899667). In the larval brain, required to maintain the self-renewal and identity of type II neuroblasts by regulating the expression of the transcriptional repressor erm together with other self-renewal transcriptional repressors such as klu and E(spl)mgamma-HLH (PubMed : 21262215, PubMed : 22357926, PubMed : 23056424, PubMed : 24618901, PubMed : 28899667). As part of its role in neuroblasts development, has been shown to be a direct target of the Notch signaling pathway, however might work also independently of N/Notch (PubMed : 21262215, PubMed : 22357926, PubMed : 23056424). In the developing larval and pupal brain, required for mushroom body differentiation (PubMed : 22357926). Involved in sex determination and SXL transcription repression when in complex with the corepressor protein Groucho (PubMed : 7651341, PubMed : 8001118).

See full target information dpn

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7930 PubMed40855028

2025

Dual mitotic bookmarking by GAF and H3K27ac orchestrates differential propagation of cell fate memory in neural development.

Applications

Unspecified application

Species

Unspecified reactive species

Rulan Zhang,Jie Liu,Zimo Zhang,Zili Chen,Tanpeng Wang,Yuying Shen,Zejun Lan,Jingyi Chu,Haoxuan Tang,Xiyue Zhang,Yan Song

PLoS biology 23:e3002881 PubMed40163536

2025

The Hunchback transcription factor determines interneuron molecular identity, morphology, and presynapse targeting in the Drosophila NB5-2 lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Heather Q Pollington,Chris Q Doe

Science advances 11:eadr6682 PubMed39919181

2025

Imp/IGF2BP and Syp/SYNCRIP temporal RNA interactomes uncover combinatorial networks of regulators of brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Y Lee,Niles Huang,Tamsin J Samuels,Ilan Davis

Genetics in medicine : official journal of the American College of Medical Genetics 26:101218 PubMed39036895

2024

Loss-of-function in RBBP5 results in a syndromic neurodevelopmental disorder associated with microcephaly.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Huang,Kristy L Jay,Alden Yen-Wen Huang,Jijun Wan,Sharayu V Jangam,Odelia Chorin,Annick Rothschild,Ortal Barel,Milena Mariani,Maria Iascone,Han Xue,Jing Huang,Cyril Mignot,Boris Keren,Virginie Saillour,Annelise Y Mah-Som,Stephanie Sacharow,Farrah Rajabi,Carrie Costin,Shinya Yamamoto,Oguz Kanca,Hugo J Bellen,Jill A Rosenfeld,Christina G S Palmer,Stanley F Nelson,Michael F Wangler,Julian A Martinez-Agosto

Fly 18:2352938 PubMed38741287

2024

A novel loss-of-function mutant in .

Applications

Unspecified application

Species

Unspecified reactive species

Nicole A Losurdo,Adriana Bibo,Jacob Bedke,Nichole Link

eLife 12: PubMed38391176

2024

Delta-dependent Notch activation closes the early neuroblast temporal program to promote lineage progression and neurogenesis termination in .

Applications

Unspecified application

Species

Unspecified reactive species

Chhavi Sood,Md Ausrafuggaman Nahid,Kendall R Branham,Matt Pahl,Susan E Doyle,Sarah E Siegrist

Experimental & molecular medicine 56:422-440 PubMed38374207

2024

Baf-mediated transcriptional regulation of teashirt is essential for the development of neural progenitor cell lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Byung Su Ko,Myeong Hoon Han,Min Jee Kwon,Dong Gon Cha,Yuri Ji,Eun Seo Park,Min Jae Jeon,Somi Kim,Kyeongho Lee,Yoon Ha Choi,Jusung Lee,Monica Torras-Llort,Ki-Jun Yoon,Hyosang Lee,Jong Kyoung Kim,Sung Bae Lee

iScience 27:108545 PubMed38213621

2024

Golgi-to-ER retrograde transport prevents premature differentiation of type II neuroblasts via Notch-signal-sending daughter cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Zhang,Menglong Rui,Zhixin Ma,Sifan Gong,Shuliu Zhang,Qingxia Zhou,Congfeng Gan,Wenting Gong,Su Wang

Cell reports 42:112181 PubMed36870055

2023

Simultaneous activation of Tor and suppression of ribosome biogenesis by TRIM-NHL proteins promotes terminal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Gui,Tamsin J Samuels,Katarina Z A Grobicki,Felipe Karam Teixeira

microPublication biology 2022: PubMed36606082

2022

Developmental origin of the Pair1 descending interneuron.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Linskens,Chris Doe,Kristen Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Deadpan antibody [11D1BC7]

Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173)

Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (37)

Product promise