Anti-Deadpan antibody [11D1BC7] (ab195173) is a rat monoclonal antibody detecting Deadpan. Suitable for Drosophila melanogaster.
- Over 20 publications
Images
![Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173), expandable thumbnail](https://content.abcam.com/products/images/deadpan-antibody-11d1bc7-ab195173--immunohistochemistry-img65741.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173)")
![Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173), expandable thumbnail](https://content.abcam.com/products/images/deadpan-antibody-11d1bc7-ab195173--immunohistochemistry-img133055.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173)")
Publications
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- microPublication biology 2022:2022Developmental origin of the Pair1 descending interneuron.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda Linskens,Chris Doe,Kristen LeePubMed 36606082
- eLife 11:2022A Notch-dependent transcriptional mechanism controls expression of temporal patterning factors in medulla.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlokananda Ray,Xin LiPubMed 36040415
- eLife 11:2022Intact central nervous system cellular quantitation reveals sexual dimorphism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Jiao,Gard Spreemann,Evelyne Ruchti,Soumya Banerjee,Samuel Vernon,Ying Shi,R Steven Stowers,Kathryn Hess,Brian D McCabePubMed 35801638
- Developmental cell 57:1193-1207.e72022NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJocelyn L Y Tang,Anna E Hakes,Robert Krautz,Takumi Suzuki,Esteban G Contreras,Paul M Fox,Andrea H BrandPubMed 35483359
- Nature communications 13:12472022A comprehensive temporal patterning gene network in Drosophila medulla neuroblasts revealed by single-cell RNA sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHailun Zhu,Sihai Dave Zhao,Alokananda Ray,Yu Zhang,Xin LiPubMed 35273186
- Development (Cambridge, England) 149:2022Notch signaling regulates neural stem cell quiescence entry and exit in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChhavi Sood,Virginia T Justis,Susan E Doyle,Sarah E SiegristPubMed 35112131
- Cell reports 37:1098952021Peripheral astral microtubules ensure asymmetric furrow positioning in neural stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre Thomas,Emmanuel Gallaud,Aude Pascal,Laurence Serre,Isabelle Arnal,Laurent Richard-Parpaillon,Matthew Scott Savoian,Régis GietPubMed 34706235
- Genetics 219:2021Neurogenesis in the adult Drosophila brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKassi L Crocker,Khailee Marischuk,Stacey A Rimkus,Hong Zhou,Jerry C P Yin,Grace Boekhoff-FalkPubMed 34117750
- eLife 10:2021A KDM5-Prospero transcriptional axis functions during early neurodevelopment to regulate mushroom body formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayden AM Hatch,Helen M Belalcazar,Owen J Marshall,Julie SecombePubMed 33729157
- Developmental cell :2021Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmmanuel Martin et. al.PubMed 33535069
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC | |
|---|---|
| Drosophila melanogaster | Tested |
IHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription (PubMed:1427077, PubMed:24618901, PubMed:28899667). In the larval brain, required to maintain the self-renewal and identity of type II neuroblasts by regulating the expression of the transcriptional repressor erm together with other self-renewal transcriptional repressors such as klu and E(spl)mgamma-HLH (PubMed:21262215, PubMed:22357926, PubMed:23056424, PubMed:24618901, PubMed:28899667). As part of its role in neuroblasts development, has been shown to be a direct target of the Notch signaling pathway, however might work also independently of N/Notch (PubMed:21262215, PubMed:22357926, PubMed:23056424). In the developing larval and pupal brain, required for mushroom body differentiation (PubMed:22357926). Involved in sex determination and SXL transcription repression when in complex with the corepressor protein Groucho (PubMed:7651341, PubMed:8001118).
Alternative names
CG8704, dpn, Protein deadpan
Recommended products
Anti-Deadpan antibody [11D1BC7] (ab195173) is a rat monoclonal antibody detecting Deadpan. Suitable for Drosophila melanogaster.
- Over 20 publications
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 11D1BC7
- Light chain type
- kappa
- Concentration
- Purification notes
The purity of ab195173 is near homogeneity, as judged by SDS-PAGE. ab195173 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.>95% based on SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-Deadpan antibody [11D1BC7] (ab195173) is a rat monoclonal antibody Trusted by the scientific community
Anti-Deadpan [11D1BC7] (ab195173) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (ab195173), expandable thumbnail](https://content.abcam.com/products/images/deadpan-antibody-11d1bc7-ab195173--immunohistochemistry-img65741.jpg "Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (ab195173)")
Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (ab195173)
Immunohistochemistry analysis using ab195173 detecting the nuclear transcription factor Deadpan protein in the neuroblasts of the third instar larval brain of Drosophila melanogaster. The brain was fixed in 4% formaldehyde (20 min) and then incubated in 1%BSA / 10% normal goat serum in 0.1% PBS-Tween for 1hr to permeabilise the cells and block non-specific protein-protein interactions. The brain was then incubated with ab195173 (10 μg/mL) overnight at +4°C and detected with Alexa Fluor® 555 goat anti-rat IgG. The counterstain was chicken anti-GFP detected with Alexa Fluor® 488 used according to manufacturer's instructions.
![Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (ab195173), expandable thumbnail](https://content.abcam.com/products/images/deadpan-antibody-11d1bc7-ab195173--immunohistochemistry-img133055.jpg "Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (ab195173)")
Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (ab195173)
Immunohistochemistry analysis using ab195173 detecting Drosophila stage 11 embryos stained showing Deadpan (Dpn) protein in all neuroblast nuclei. The Deadpan stain used ab195173 at 10 μg/mL and detected with Alexa Fluor® 405 conjugated goat anti-rat (at 10μg/mL).
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